Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABVN21_RS27395 Genome accession   NZ_CP159258
Coordinates   6041769..6042188 (+) Length   139 a.a.
NCBI ID   WP_339555080.1    Uniprot ID   A0AAU8E2M4
Organism   Pseudomonas sp. MYb327     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6036769..6047188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABVN21_RS27360 (ABVN21_27360) - 6036845..6037471 (-) 627 WP_007970534.1 DUF1780 domain-containing protein -
  ABVN21_RS27365 (ABVN21_27365) - 6037609..6038298 (+) 690 WP_339555076.1 energy-coupling factor ABC transporter permease -
  ABVN21_RS27370 (ABVN21_27370) yacG 6038324..6038524 (-) 201 WP_060543361.1 DNA gyrase inhibitor YacG -
  ABVN21_RS27375 (ABVN21_27375) coaE 6038521..6039144 (-) 624 WP_339555077.1 dephospho-CoA kinase -
  ABVN21_RS27380 (ABVN21_27380) pilD 6039141..6040013 (-) 873 WP_339555078.1 A24 family peptidase Machinery gene
  ABVN21_RS27385 (ABVN21_27385) pilC 6040015..6041232 (-) 1218 WP_339555079.1 type II secretion system F family protein Machinery gene
  ABVN21_RS27390 (ABVN21_27390) - 6041235..6041537 (-) 303 Protein_5388 type IV-A pilus assembly ATPase PilB -
  ABVN21_RS27395 (ABVN21_27395) pilA 6041769..6042188 (+) 420 WP_339555080.1 pilin Machinery gene
  ABVN21_RS27400 (ABVN21_27400) - 6042496..6042849 (+) 354 WP_339555081.1 BON domain-containing protein -
  ABVN21_RS27405 (ABVN21_27405) - 6043063..6046017 (-) 2955 WP_339555082.1 DUF748 domain-containing protein -
  ABVN21_RS27410 (ABVN21_27410) - 6046111..6047127 (-) 1017 WP_339555083.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14304.43 Da        Isoelectric Point: 7.5886

>NTDB_id=1012953 ABVN21_RS27395 WP_339555080.1 6041769..6042188(+) (pilA) [Pseudomonas sp. MYb327]
MNNQKGFTLIELLIVVAIIGILATFAIPAYSKYQARAKVTAGLAEISALKVPFEDVLNQGTDPDLTKIGGTATTSNCTTT
ASGKAADGTGTIACTILNAPGPVLNKTITLNRTSTGWTCDTGVDQDYAPKGCTGVVPKV

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1012953 ABVN21_RS27395 WP_339555080.1 6041769..6042188(+) (pilA) [Pseudomonas sp. MYb327]
ATGAACAATCAAAAAGGTTTTACCCTGATCGAGCTGCTAATCGTGGTGGCGATCATCGGGATTCTGGCGACGTTTGCGAT
CCCGGCTTATTCGAAGTATCAGGCACGGGCCAAGGTGACGGCGGGGCTGGCGGAGATCTCGGCGTTGAAGGTGCCGTTTG
AAGACGTGCTGAATCAAGGGACCGATCCAGATCTGACCAAAATTGGCGGTACAGCGACGACCTCCAACTGCACCACGACG
GCTTCGGGCAAGGCGGCTGACGGTACGGGCACCATCGCCTGCACGATTCTCAACGCACCGGGTCCTGTATTGAACAAAAC
CATTACCTTGAACCGTACATCGACGGGCTGGACATGCGACACCGGCGTCGATCAGGACTACGCGCCCAAAGGCTGCACTG
GCGTTGTTCCGAAGGTCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

47.183

100

0.482
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.905

98.561

0.482
  pilA Pseudomonas aeruginosa PAK

42.282

100

0.453
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.096

100

0.432
  pilA Vibrio cholerae strain A1552

41.096

100

0.432
  pilA Vibrio cholerae C6706

41.096

100

0.432
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.444

97.122

0.432
  pilA2 Legionella pneumophila str. Paris

42.647

97.842

0.417
  pilA2 Legionella pneumophila strain ERS1305867

41.912

97.842

0.41
  comP Acinetobacter baylyi ADP1

38.095

100

0.403

Multiple sequence alignment