Detailed information NTG1012927

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ABVN21_RS07500
• Genome accession: NZ_CP159258
• Coordinates: 1665493..1666602 (+)
• Length: 369 a.a.
• NCBI ID: WP_339556654.1
• Uniprot ID: -
• Organism: Pseudomonas sp. MYb327
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1660493..1671602

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABVN21_RS07485 (ABVN21_07485)	-	1661461..1662393 (+)	933	WP_339556652.1	sugar kinase	-
ABVN21_RS07490 (ABVN21_07490)	-	1662508..1662783 (-)	276	WP_007933743.1	peptidylprolyl isomerase	-
ABVN21_RS07495 (ABVN21_07495)	-	1662838..1665375 (-)	2538	WP_339556653.1	PAS domain S-box protein	-
ABVN21_RS07500 (ABVN21_07500)	pilU	1665493..1666602 (+)	1110	WP_339556654.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ABVN21_RS07505 (ABVN21_07505)	-	1666700..1668655 (-)	1956	WP_339556655.1	acetoacetate--CoA ligase	-
ABVN21_RS07510 (ABVN21_07510)	hbdH	1668809..1669582 (-)	774	WP_034147156.1	3-hydroxybutyrate dehydrogenase	-
ABVN21_RS07515 (ABVN21_07515)	-	1669590..1670981 (-)	1392	WP_339556656.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40925.85 Da
• Isoelectric Point: 6.6443

>NTDB_id=1012927 ABVN21_RS07500 WP_339556654.1 1665493..1666602(+) (pilU) [Pseudomonas sp. MYb327]
MEIDALLRRLASQDGSDLYLSTGAPPSARFDGVLQSLADQTFKPGEIATMAASIMDAEQRLEFDRELEMNLAISLPGIGR
FRVNIFKQRNDVSMVVRNVKLDIPRFEDLKLPSVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHSHGHIVTIED
PIEYVHRHKKSVINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADSGHLVISTLHAHNANQALNRI
INFFPEERRGQLLNDLGNNLKAFVSQRLVRTLDGQRRVAAEVMLGTPTIGDLIRRNEFSELKAVMEKSAALGMQTFDAAL
YALVLEGAIDESQALKHADSVNNLRLRLKLHAEALPGANAVSGEWGLLD

Nucleotide

• Length: 1110 bp

>NTDB_id=1012927 ABVN21_RS07500 WP_339556654.1 1665493..1666602(+) (pilU) [Pseudomonas sp. MYb327]
ATGGAAATCGATGCGTTATTAAGACGCCTGGCCAGCCAGGATGGCTCTGACCTTTATCTGTCCACGGGTGCCCCTCCCAG
TGCGCGATTCGACGGTGTACTTCAATCCCTTGCAGATCAGACGTTCAAGCCCGGGGAAATTGCGACAATGGCCGCGTCCA
TCATGGACGCCGAACAGCGTCTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCGATCTCCCTGCCGGGCATCGGCCGC
TTTCGGGTCAATATTTTCAAACAGCGCAACGATGTGTCGATGGTGGTGCGCAATGTCAAACTCGACATTCCACGCTTCGA
AGACCTCAAGTTGCCCTCGGTGTTGCTCGAAACGGTCATGCTCAAACAAGGCTTGATGCTGTTCGTCGGCGCCACTGATT
CGGGCAAATCGACCTCGTTGGCGGCGTTGATCGACTATCGCAACCGCCACAGCCATGGGCATATCGTCACTATTGAAGAC
CCCATCGAGTACGTTCACCGGCACAAAAAATCGGTCATCAATCAGCGCGAGGTCGGCGTCGACACCCGTAGTTTTCATGC
GGCGTTGAAAAACACCTTGCGCCAGGCGCCGGATGTGGTGCTGATCGGCGAGATCCGCGACCGGGAAACCATGGAGCACG
CACTGGCGTTCGCCGATTCCGGGCATCTGGTGATCTCTACACTGCACGCCCACAATGCTAATCAGGCGCTAAACCGCATC
ATCAACTTCTTCCCCGAAGAACGCCGGGGTCAACTTCTCAATGATCTGGGCAACAACCTCAAGGCTTTTGTTTCACAGCG
TTTGGTACGCACCCTCGATGGCCAGCGACGTGTGGCAGCTGAAGTGATGCTGGGCACGCCGACCATTGGCGATTTGATTC
GGCGTAACGAGTTTTCCGAGCTGAAAGCGGTGATGGAAAAGTCGGCGGCGTTGGGGATGCAGACGTTTGATGCCGCGTTG
TATGCGCTGGTGCTGGAAGGGGCGATTGATGAGTCGCAGGCGTTGAAACACGCGGATTCGGTGAACAATTTGCGCTTGCG
GTTGAAGTTGCATGCCGAGGCTTTGCCCGGTGCGAATGCAGTATCCGGCGAATGGGGGCTGCTGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.648	96.206	0.526
pilU	Acinetobacter baylyi ADP1	51.124	96.477	0.493
pilU	Vibrio cholerae strain A1552	50.286	94.851	0.477
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.541	90.244	0.366
pilT	Vibrio cholerae strain A1552	40.541	90.244	0.366
pilT	Pseudomonas stutzeri DSM 10701	39.244	93.225	0.366
pilT	Neisseria gonorrhoeae MS11	39.13	93.496	0.366
pilT	Neisseria meningitidis 8013	39.589	92.412	0.366
pilT	Pseudomonas aeruginosa PAK	38.953	93.225	0.363
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.233	91.87	0.36
pilT	Legionella pneumophila strain ERS1305867	39.82	90.515	0.36
pilT	Legionella pneumophila strain Lp02	39.82	90.515	0.36