Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ABNK63_RS04465 Genome accession   NZ_CP157948
Coordinates   1026708..1027133 (-) Length   141 a.a.
NCBI ID   WP_350016811.1    Uniprot ID   A0AAU7QMR2
Organism   Rhodanobacter sp. IGA1.0     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1006953..1031066 1026708..1027133 within 0


Gene organization within MGE regions


Location: 1006953..1031066
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABNK63_RS04385 (ABNK63_04380) - 1006953..1007345 (-) 393 WP_007809537.1 endonuclease domain-containing protein -
  ABNK63_RS04390 (ABNK63_04385) pilC 1007402..1008673 (-) 1272 WP_007809536.1 type II secretion system F family protein Machinery gene
  ABNK63_RS04395 (ABNK63_04390) pilB 1008816..1010540 (-) 1725 WP_350016799.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABNK63_RS04400 (ABNK63_04395) - 1010888..1011130 (+) 243 WP_007809534.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  ABNK63_RS04405 (ABNK63_04400) - 1011127..1011522 (+) 396 WP_007809533.1 type II toxin-antitoxin system VapC family toxin -
  ABNK63_RS04410 (ABNK63_04405) - 1011854..1012708 (+) 855 WP_350016800.1 glycosyltransferase family 2 protein -
  ABNK63_RS04415 (ABNK63_04410) - 1012701..1013927 (+) 1227 WP_350016801.1 glycosyltransferase -
  ABNK63_RS04420 (ABNK63_04415) - 1014119..1014817 (+) 699 WP_350016802.1 class I SAM-dependent methyltransferase -
  ABNK63_RS04425 (ABNK63_04420) - 1014954..1015883 (-) 930 WP_350016803.1 glycosyltransferase -
  ABNK63_RS04430 (ABNK63_04425) - 1015889..1017034 (-) 1146 WP_350016804.1 glycosyltransferase family 4 protein -
  ABNK63_RS04435 (ABNK63_04430) asnB 1017034..1018971 (-) 1938 WP_350016805.1 asparagine synthase (glutamine-hydrolyzing) -
  ABNK63_RS04440 (ABNK63_04435) - 1018974..1020071 (-) 1098 WP_350016806.1 glycosyltransferase -
  ABNK63_RS04445 (ABNK63_04440) - 1020068..1020874 (-) 807 WP_350016807.1 methyltransferase domain-containing protein -
  ABNK63_RS04450 (ABNK63_04445) - 1020864..1022024 (-) 1161 WP_350016808.1 oligosaccharide flippase family protein -
  ABNK63_RS04455 (ABNK63_04450) - 1022311..1024419 (-) 2109 WP_350016809.1 hypothetical protein -
  ABNK63_RS04460 (ABNK63_04455) - 1024652..1026607 (-) 1956 WP_350016810.1 hypothetical protein -
  ABNK63_RS04465 (ABNK63_04460) pilA 1026708..1027133 (-) 426 WP_350016811.1 pilin Machinery gene
  ABNK63_RS04470 (ABNK63_04465) - 1027578..1028006 (-) 429 WP_350016812.1 pilin -
  ABNK63_RS04475 (ABNK63_04470) - 1028205..1030337 (+) 2133 WP_350016813.1 S9 family peptidase -
  ABNK63_RS04480 (ABNK63_04475) - 1030776..1031066 (+) 291 WP_350016814.1 nucleotidyltransferase domain-containing protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14872.14 Da        Isoelectric Point: 8.5024

>NTDB_id=1009312 ABNK63_RS04465 WP_350016811.1 1026708..1027133(-) (pilA) [Rhodanobacter sp. IGA1.0]
MKTMQKGFTLIELMIVVAIIAILAAIALPQYQNYVARSQATAGLADITPGKTAFEEMVNNGQSAANFTVDAVGLQATTER
CAVALTQPDATGAAAAALKCTLKGNPKVATKTIQWDRASNGSWTCHSSLEDKFRPKGCVNP

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1009312 ABNK63_RS04465 WP_350016811.1 1026708..1027133(-) (pilA) [Rhodanobacter sp. IGA1.0]
ATGAAGACCATGCAGAAAGGTTTTACCCTGATCGAACTGATGATCGTGGTTGCGATCATCGCGATCCTGGCCGCCATCGC
GCTGCCGCAGTACCAGAACTATGTGGCTCGTTCACAGGCGACTGCCGGCTTGGCAGATATCACGCCCGGAAAGACTGCTT
TCGAGGAAATGGTCAATAATGGCCAATCTGCTGCTAATTTCACTGTTGATGCAGTGGGCCTGCAGGCGACTACCGAGCGT
TGCGCCGTAGCTCTCACACAGCCGGATGCCACTGGCGCTGCTGCCGCAGCACTCAAGTGCACCCTCAAGGGCAACCCCAA
GGTTGCAACCAAGACGATCCAGTGGGATCGTGCCAGCAATGGTTCCTGGACCTGCCACAGCTCGCTGGAAGACAAGTTCC
GTCCCAAGGGTTGCGTGAACCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

51.799

98.582

0.511
  pilA Pseudomonas aeruginosa PAK

47.586

100

0.489
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.921

98.582

0.482
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.357

100

0.44
  pilA Vibrio cholerae C6706

41.549

100

0.418
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.549

100

0.418
  pilA Vibrio cholerae strain A1552

41.549

100

0.418
  pilA2 Legionella pneumophila str. Paris

41.007

98.582

0.404
  pilA2 Legionella pneumophila strain ERS1305867

40.288

98.582

0.397
  pilA Ralstonia pseudosolanacearum GMI1000

35.256

100

0.39
  comP Acinetobacter baylyi ADP1

36.62

100

0.369

Multiple sequence alignment