Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   QUD85_RS10795 Genome accession   NZ_AP027363
Coordinates   2372790..2373917 (-) Length   375 a.a.
NCBI ID   WP_093331810.1    Uniprot ID   A0A1I0HGX7
Organism   Thalassotalea agarivorans strain JCM 13379     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2367790..2378917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD85_RS10775 cydB 2368125..2369123 (-) 999 WP_093331804.1 cytochrome d ubiquinol oxidase subunit II -
  QUD85_RS10780 - 2369120..2370520 (-) 1401 WP_218139606.1 cytochrome ubiquinol oxidase subunit I -
  QUD85_RS10785 - 2370592..2371137 (-) 546 WP_093331805.1 GbsR/MarR family transcriptional regulator -
  QUD85_RS10790 - 2372070..2372504 (-) 435 WP_093331807.1 hypothetical protein -
  QUD85_RS10795 pilU 2372790..2373917 (-) 1128 WP_093331810.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  QUD85_RS10800 pilT 2373930..2374973 (-) 1044 WP_093331813.1 type IV pilus twitching motility protein PilT Machinery gene
  QUD85_RS10805 - 2375124..2375795 (+) 672 WP_093331815.1 YggS family pyridoxal phosphate-dependent enzyme -
  QUD85_RS10810 proC 2375792..2376613 (+) 822 WP_093331818.1 pyrroline-5-carboxylate reductase -
  QUD85_RS10815 - 2376629..2377180 (+) 552 WP_093331820.1 YggT family protein -
  QUD85_RS10820 - 2377259..2377702 (+) 444 WP_407705066.1 DUF4426 domain-containing protein -
  QUD85_RS10825 - 2377800..2378387 (+) 588 WP_093331889.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41597.70 Da        Isoelectric Point: 4.8440

>NTDB_id=100841 QUD85_RS10795 WP_093331810.1 2372790..2373917(-) (pilU) [Thalassotalea agarivorans strain JCM 13379]
MLSLNDILRIMVEKDASDVFVTAKLPVSAKVNGELTPIDQESLTPEQAIALVEDAMNDKQKLQFHEEKECNFAISIDEIG
RFRVSAFWQRDMAGMVIRRIVTEIPDVEELGLPPVLKEVVMSKRGLVLFVGGTGTGKSTSMAALIGYRNRNSRGHILTIE
DPVEFVHEHAQSMITQREVGLDTESFDAALKSSLRQAPDVILIGEIRNQETMEFALSFAETGHLCIATLHANNANQAIDR
IMHLVPAEQHGKLLFDLALNLRGIVAQQLIPTRDGHSRVAAIEVLLNSPYIAELIKKGEVGDIKEVMQKSNELGMMTFDQ
ALFNLYQRGLISYADALHHADSPNDLRLMIKLRSNEQDGVGSLKGVTLDGMEDEF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=100841 QUD85_RS10795 WP_093331810.1 2372790..2373917(-) (pilU) [Thalassotalea agarivorans strain JCM 13379]
ATGCTTTCTTTAAACGATATACTCCGCATCATGGTAGAGAAAGACGCGTCTGATGTCTTTGTCACTGCCAAGTTACCTGT
GAGTGCCAAAGTTAATGGTGAGTTAACGCCAATTGACCAAGAATCTTTAACGCCAGAGCAAGCGATTGCACTTGTTGAAG
ATGCAATGAATGACAAGCAAAAGCTCCAATTTCACGAAGAGAAAGAGTGTAACTTTGCAATTTCAATAGATGAAATCGGT
CGATTCAGGGTCTCTGCCTTCTGGCAACGAGATATGGCGGGTATGGTTATTCGTCGTATCGTTACTGAAATTCCTGATGT
AGAAGAGTTAGGCTTGCCACCTGTACTAAAAGAAGTGGTGATGTCTAAACGTGGCCTTGTACTTTTCGTTGGTGGTACCG
GTACCGGTAAATCGACCTCGATGGCGGCGTTAATTGGCTATCGTAATCGTAATTCTCGCGGTCATATTTTGACTATTGAA
GACCCGGTAGAATTTGTTCATGAACATGCGCAGTCAATGATCACACAACGTGAAGTTGGACTTGATACTGAAAGCTTTGA
TGCGGCGCTGAAAAGTTCACTTCGTCAGGCGCCAGACGTTATCCTAATCGGTGAGATTCGTAATCAAGAAACCATGGAAT
TTGCATTAAGTTTCGCAGAAACGGGCCACTTGTGTATTGCTACGCTTCACGCCAACAACGCAAACCAAGCAATTGACCGT
ATCATGCACTTAGTACCAGCAGAGCAGCATGGTAAATTGTTATTTGATTTGGCCTTGAACCTACGTGGTATCGTGGCACA
GCAGCTAATTCCTACGCGTGATGGTCACTCTCGTGTTGCTGCGATTGAAGTACTGCTTAATTCGCCGTATATCGCTGAAC
TCATCAAGAAAGGTGAGGTGGGTGATATCAAGGAAGTGATGCAAAAATCAAATGAGCTTGGCATGATGACCTTTGACCAA
GCCTTATTTAACTTGTATCAACGTGGCCTAATTAGTTATGCCGATGCGCTTCATCATGCTGATTCTCCGAACGATCTTCG
TCTAATGATCAAATTACGTAGTAATGAACAAGACGGTGTTGGTTCGCTTAAAGGTGTTACCTTAGATGGTATGGAAGATG
AGTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1I0HGX7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

59.574

100

0.597
  pilU Acinetobacter baylyi ADP1

59.091

93.867

0.555
  pilU Vibrio cholerae strain A1552

56.25

98.133

0.552
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.5

96

0.408
  pilT Pseudomonas aeruginosa PAK

41.159

92

0.379
  pilT Acinetobacter baumannii D1279779

40.058

91.2

0.365
  pilT Acinetobacter nosocomialis M2

40.058

91.2

0.365
  pilT Acinetobacter baumannii strain A118

40.058

91.2

0.365
  pilT Legionella pneumophila strain ERS1305867

41.088

88.267

0.363
  pilT Legionella pneumophila strain Lp02

41.088

88.267

0.363
  pilT Acinetobacter baylyi ADP1

39.766

91.2

0.363
  pilT Pseudomonas stutzeri DSM 10701

39.42

92

0.363

Multiple sequence alignment