Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABL840_RS35420 Genome accession   NZ_CP157613
Coordinates   7564298..7565434 (+) Length   378 a.a.
NCBI ID   WP_093206425.1    Uniprot ID   A0A1H7VSN0
Organism   Variovorax sp. NFACC27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7559298..7570434
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABL840_RS35390 (ABL840_35295) - 7559307..7560581 (-) 1275 WP_093206430.1 Glu/Leu/Phe/Val family dehydrogenase -
  ABL840_RS35395 (ABL840_35300) - 7560814..7561140 (+) 327 WP_093206429.1 DUF4148 domain-containing protein -
  ABL840_RS35400 (ABL840_35305) - 7561276..7561740 (+) 465 WP_093306955.1 hypothetical protein -
  ABL840_RS35405 (ABL840_35310) - 7561879..7562346 (+) 468 WP_019657564.1 Lrp/AsnC ligand binding domain-containing protein -
  ABL840_RS35410 (ABL840_35315) - 7562362..7563066 (-) 705 WP_093306954.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABL840_RS35415 (ABL840_35320) pilT 7563118..7564161 (+) 1044 WP_093206426.1 type IV pilus twitching motility protein PilT Machinery gene
  ABL840_RS35420 (ABL840_35325) pilU 7564298..7565434 (+) 1137 WP_093206425.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABL840_RS35425 (ABL840_35330) - 7565440..7566993 (-) 1554 WP_093306953.1 MFS transporter -
  ABL840_RS35430 (ABL840_35335) - 7566990..7567472 (-) 483 WP_093306952.1 MerR family transcriptional regulator -
  ABL840_RS35435 (ABL840_35340) - 7567560..7568468 (+) 909 WP_093306951.1 NAD(P)-dependent oxidoreductase -
  ABL840_RS35440 (ABL840_35345) - 7568539..7569360 (-) 822 WP_093306950.1 BON domain-containing protein -
  ABL840_RS35445 (ABL840_35350) - 7569357..7569968 (-) 612 WP_093306949.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 378 a.a.        Molecular weight: 41794.92 Da        Isoelectric Point: 6.4175

>NTDB_id=1007696 ABL840_RS35420 WP_093206425.1 7564298..7565434(+) (pilU) [Variovorax sp. NFACC27]
MERDQASQFINDLLKLMVSRNGSDLFITADFPPAIKVDGKVTKVSQQALGAPHTLALTRSIMNDRQTAEFERTKECNFAI
SPTGIGRFRVNAFVQQGKVGMVLRTIPAKLPTIDGLGMPQVLKDVSMTKRGLCILVGATGSGKSTTLAAMIDWRNENSFG
HIVTVEDPVEFVHPHKNCVVTQREVGIDTDSWEAALKNTLRQAPDVILMGEIRDRETMEHAVAFAETGHLCMATLHANSA
NQALDRIINFFPEERRAQLLMDLSLNLRSLVSQRLVPTEDGLGRVAAVEVLLNTPLISDLIFKGEVGEIKEIMRKSRNLG
MQTFDQALFDLFESHSISFEDAIRNADSANDLRLQIKLNSQRARSTDLAAGTEHFAIV

Nucleotide


Download         Length: 1137 bp        

>NTDB_id=1007696 ABL840_RS35420 WP_093206425.1 7564298..7565434(+) (pilU) [Variovorax sp. NFACC27]
ATGGAACGCGATCAAGCCAGCCAGTTCATCAACGACCTGCTCAAGCTCATGGTGAGCCGCAACGGCAGCGACCTGTTCAT
CACGGCCGACTTTCCGCCGGCCATCAAGGTCGACGGCAAGGTCACCAAGGTTTCGCAGCAGGCGCTGGGCGCGCCGCACA
CGCTGGCGCTCACCCGCTCGATCATGAACGACCGCCAGACGGCCGAATTCGAGCGCACCAAGGAATGCAACTTCGCGATC
TCGCCGACCGGCATCGGCCGCTTCCGCGTGAACGCCTTCGTGCAGCAGGGCAAGGTCGGCATGGTGCTGCGGACCATTCC
CGCCAAGCTGCCGACCATCGACGGCCTGGGCATGCCGCAGGTGCTGAAGGACGTGTCGATGACCAAGCGCGGCCTGTGCA
TCCTGGTGGGTGCCACGGGCTCGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTGGCGCAACGAGAACTCGTTCGGC
CACATCGTGACGGTGGAAGACCCGGTCGAATTCGTGCATCCGCACAAGAACTGCGTGGTGACGCAGCGCGAGGTGGGCAT
CGACACCGACAGCTGGGAGGCCGCGCTGAAGAACACGCTGCGCCAGGCGCCCGACGTGATCCTGATGGGCGAAATCCGCG
ACCGCGAGACCATGGAACACGCCGTGGCCTTCGCCGAAACGGGCCACCTGTGCATGGCAACGCTGCACGCCAACAGCGCC
AACCAGGCGCTGGACCGGATCATCAACTTCTTCCCCGAAGAGCGCCGCGCCCAGCTGCTGATGGACCTGTCGCTGAACCT
GCGTTCGCTGGTGTCGCAGCGGCTGGTGCCGACGGAAGACGGCCTGGGCCGCGTGGCCGCGGTCGAAGTGCTGCTGAACA
CGCCGCTGATTTCCGACCTGATCTTCAAGGGCGAGGTGGGCGAGATCAAGGAGATCATGCGCAAGAGCCGCAACCTGGGC
ATGCAGACCTTCGACCAGGCGCTGTTCGACCTGTTCGAGAGCCACTCGATCAGCTTCGAGGACGCCATCCGCAACGCCGA
CTCGGCCAACGACCTGCGCCTGCAGATCAAGCTCAACAGCCAGCGTGCGCGCAGCACCGACCTGGCCGCGGGCACGGAGC
ACTTCGCGATCGTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(101-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H7VSN0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

63.61

92.328

0.587
  pilU Acinetobacter baylyi ADP1

57.821

94.709

0.548
  pilU Vibrio cholerae strain A1552

53.168

96.032

0.511
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.401

89.153

0.405
  pilT Pseudomonas aeruginosa PAK

45.104

89.153

0.402
  pilT Acinetobacter nosocomialis M2

43.844

88.095

0.386
  pilT Acinetobacter baumannii D1279779

43.844

88.095

0.386
  pilT Acinetobacter baumannii strain A118

43.844

88.095

0.386
  pilT Pseudomonas stutzeri DSM 10701

43.027

89.153

0.384
  pilT Legionella pneumophila strain Lp02

43.769

87.037

0.381
  pilT Legionella pneumophila strain ERS1305867

43.769

87.037

0.381
  pilT Acinetobacter baylyi ADP1

42.342

88.095

0.373

Multiple sequence alignment