Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAG688_RS04195 Genome accession   NZ_CP157201
Coordinates   936957..938096 (-) Length   379 a.a.
NCBI ID   WP_411834101.1    Uniprot ID   -
Organism   Pseudoxanthomonas mexicana strain SDU_PSEU2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 931957..943096
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG688_RS04160 coq7 931984..932628 (-) 645 WP_411834094.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  AAG688_RS04165 rplM 932855..933283 (+) 429 WP_411834096.1 50S ribosomal protein L13 -
  AAG688_RS04170 rpsI 933286..933678 (+) 393 WP_411834097.1 30S ribosomal protein S9 -
  AAG688_RS04180 - 933889..934659 (+) 771 WP_411834098.1 2OG-Fe dioxygenase family protein -
  AAG688_RS04185 - 934706..935692 (+) 987 Protein_819 fumarylacetoacetate hydrolase family protein -
  AAG688_RS04190 maiA 935776..936438 (+) 663 WP_411834100.1 maleylacetoacetate isomerase -
  AAG688_RS04195 pilU 936957..938096 (-) 1140 WP_411834101.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAG688_RS04200 - 938190..938579 (+) 390 WP_411834102.1 DUF4398 domain-containing protein -
  AAG688_RS04205 - 938576..939466 (+) 891 WP_411834104.1 OmpA family protein -
  AAG688_RS04210 - 939825..940043 (+) 219 WP_411834105.1 YdcH family protein -
  AAG688_RS04215 - 940165..941535 (+) 1371 WP_411834106.1 pyridoxal-phosphate dependent enzyme -
  AAG688_RS04220 - 941633..942805 (+) 1173 WP_411834107.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 41713.94 Da        Isoelectric Point: 6.1372

>NTDB_id=1004571 AAG688_RS04195 WP_411834101.1 936957..938096(-) (pilU) [Pseudoxanthomonas mexicana strain SDU_PSEU2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPVFERELELNMAIALADAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELNLPQVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDYRNSNTTGHILTIED
PIEYLHRHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGVDGRRLPAAEVLINTPMIRDLLRRGQVHEIKQAMEASLEEGMESFDQCL
FRMVKEGRIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADYSGGAGGSSQITHGFG

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=1004571 AAG688_RS04195 WP_411834101.1 936957..938096(-) (pilU) [Pseudoxanthomonas mexicana strain SDU_PSEU2]
ATGGACATCGGCTATTTCCTCAAGCTGATGACCGAGAAGAACGCCTCGGACATGTTCCTGACCACCGGGGCCCCGGTCTA
CATCAAGATTGAAGGCAAGCTGTACCCGCTGGGCAACACCGGCCTGCCCCCGGGCATGGTGAAGAAGATCGCCTATTCGC
TGATGGACGAGGGCCAGGTGCCGGTGTTCGAGCGCGAACTGGAGCTGAACATGGCGATCGCGCTGGCCGACGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGCTGGTCGGCTCCACCGGCT
CGGGCAAGTCCACCTCGCTGGCGTCGATGATCGACTACCGCAACAGCAACACCACCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACCGGCACAAGCAGTCGATCGTCAACCAGCGCGAGGTCGGCCTGGACACCCACGCCTTCCACAA
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTCATCCTGATCGGCGAGATCCTCGACGCCACCACGATGGAGGCGG
CCATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCCGAGAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTCTCGCAGCG
CCTGGTCAAGGGCGTGGACGGCCGCCGCCTGCCCGCCGCCGAGGTGTTGATCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAGGTGCACGAGATCAAGCAGGCGATGGAGGCCTCGCTGGAGGAAGGCATGGAGTCGTTCGACCAGTGCCTG
TTCCGGATGGTCAAGGAAGGCCGCATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
GTTCCGACTGTCCGAGGGCTCCAGCGGCGAGCACGACCCCTACGCCGACTACAGCGGCGGCGCGGGCGGTTCGTCGCAGA
TCACCCACGGCTTCGGCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.811

94.723

0.491
  pilU Acinetobacter baylyi ADP1

48.588

93.404

0.454
  pilU Vibrio cholerae strain A1552

46.612

97.361

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.972

94.459

0.425
  pilT Acinetobacter baumannii strain A118

41.42

89.182

0.369
  pilT Acinetobacter nosocomialis M2

41.42

89.182

0.369
  pilT Acinetobacter baumannii D1279779

41.42

89.182

0.369
  pilT Pseudomonas aeruginosa PAK

41.42

89.182

0.369
  pilT Acinetobacter baylyi ADP1

40.588

89.71

0.364
  pilT Legionella pneumophila strain Lp02

40.828

89.182

0.364
  pilT Legionella pneumophila strain ERS1305867

40.828

89.182

0.364
  pilT Pseudomonas stutzeri DSM 10701

40.533

89.182

0.361

Multiple sequence alignment