Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CV024_RS14255 Genome accession   NZ_CP156745
Coordinates   3072380..3073486 (-) Length   368 a.a.
NCBI ID   WP_100268561.1    Uniprot ID   -
Organism   Vibrio fluvialis strain QY27     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3067380..3078486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV024_RS14225 (CV024_14225) - 3068108..3068608 (+) 501 WP_100268560.1 SprT family zinc-dependent metalloprotease -
  CV024_RS14230 (CV024_14230) - 3068727..3069428 (+) 702 WP_032080466.1 endonuclease -
  CV024_RS14235 (CV024_14235) rsmE 3069525..3070256 (+) 732 WP_024374567.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CV024_RS14240 (CV024_14240) gshB 3070269..3071225 (+) 957 WP_024374566.1 glutathione synthase -
  CV024_RS14245 (CV024_14245) - 3071265..3071828 (+) 564 WP_032080468.1 YqgE/AlgH family protein -
  CV024_RS14250 (CV024_14250) ruvX 3071890..3072315 (+) 426 WP_024374565.1 Holliday junction resolvase RuvX -
  CV024_RS14255 (CV024_14255) pilU 3072380..3073486 (-) 1107 WP_100268561.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CV024_RS14260 (CV024_14260) pilT 3073498..3074535 (-) 1038 WP_024374563.1 type IV pilus twitching motility protein PilT Machinery gene
  CV024_RS14265 (CV024_14265) - 3074561..3075268 (+) 708 WP_100268562.1 YggS family pyridoxal phosphate-dependent enzyme -
  CV024_RS14270 (CV024_14270) proC 3075305..3076123 (+) 819 WP_100268563.1 pyrroline-5-carboxylate reductase -
  CV024_RS14275 (CV024_14275) - 3076175..3076732 (+) 558 WP_024374560.1 YggT family protein -
  CV024_RS14280 (CV024_14280) yggU 3076732..3077022 (+) 291 WP_024374559.1 DUF167 family protein YggU -
  CV024_RS14285 (CV024_14285) - 3077082..3077513 (+) 432 WP_024374558.1 DUF4426 domain-containing protein -
  CV024_RS14290 (CV024_14290) - 3077537..3078136 (+) 600 WP_100268564.1 XTP/dITP diphosphatase -

Sequence

Protein


Download         Length: 368 a.a.        Molecular weight: 41187.18 Da        Isoelectric Point: 6.3195

>NTDB_id=1002473 CV024_RS14255 WP_100268561.1 3072380..3073486(-) (pilU) [Vibrio fluvialis strain QY27]
MQLEECLQGMMTHKASDLYITVGAPVLYRVDGELRAKGEKLDEAQVSALLAAMMDEARHADYLATREANFAIVRDLGRFR
VSAFFQRELPGAVIRRIETQIPTFDELKLPAVLKDLSIAKRGLVLVVGATGSGKSTTMAAMTGYRNTHRSGHILTVEDPI
EFVHEHQRCIVTQREVGLDTESYEVALKNSLRQAPDMILIGEIRSRETMEYAMTFAETGHLCMATLHANNANQALERILH
LVPKEQREQFLFDLSMNLKGVVGQQLIRDKNGQGRHGVFEVLLNSPRVSDIIRRGDLHELKSTMAKSKEVGMQTFDQALY
QLVVENKISEQDALHCADSANDLRLMLKTQRGDVTSSGSLDHVQIDME

Nucleotide


Download         Length: 1107 bp        

>NTDB_id=1002473 CV024_RS14255 WP_100268561.1 3072380..3073486(-) (pilU) [Vibrio fluvialis strain QY27]
ATGCAACTCGAGGAGTGCCTGCAAGGCATGATGACGCATAAAGCGTCAGATCTATATATTACTGTGGGCGCCCCCGTACT
GTACCGGGTCGATGGTGAACTGCGCGCGAAGGGTGAGAAGCTGGATGAAGCGCAGGTCAGCGCGCTGCTGGCTGCGATGA
TGGATGAAGCGCGCCATGCGGACTATCTGGCGACGCGTGAAGCTAACTTTGCCATCGTGCGTGATTTGGGGCGTTTTCGC
GTCAGCGCTTTTTTTCAGCGCGAGCTACCCGGTGCGGTGATTCGCCGCATCGAAACGCAGATTCCGACCTTTGATGAACT
CAAACTGCCAGCGGTGCTGAAAGATCTCTCGATTGCCAAACGCGGTTTAGTGCTGGTGGTTGGGGCGACAGGGTCGGGCA
AATCGACCACCATGGCCGCGATGACTGGCTATCGCAACACGCACCGCAGCGGGCATATTCTCACCGTCGAAGATCCGATT
GAATTTGTCCATGAGCACCAGCGCTGCATCGTGACGCAGCGCGAAGTGGGATTGGACACCGAAAGCTATGAAGTGGCGCT
GAAGAACTCGCTACGCCAGGCGCCGGATATGATTTTGATCGGTGAGATCCGCAGCCGTGAAACCATGGAATACGCAATGA
CCTTCGCCGAAACCGGTCACTTATGCATGGCAACTTTGCACGCCAACAACGCCAACCAAGCGCTCGAACGTATTTTGCAT
CTGGTGCCGAAAGAGCAGCGTGAACAATTTCTGTTTGACCTGTCGATGAACCTCAAAGGTGTTGTCGGTCAGCAACTGAT
CCGCGATAAAAACGGCCAGGGACGTCACGGCGTGTTTGAAGTGTTGCTCAACAGTCCACGGGTGTCGGACATCATCCGAC
GCGGCGATTTGCATGAGCTGAAAAGCACCATGGCGAAATCGAAAGAGGTTGGCATGCAGACTTTTGATCAGGCGTTGTAT
CAGTTGGTGGTCGAGAACAAGATCAGCGAACAGGATGCGCTGCACTGTGCCGATTCGGCCAACGATCTGCGTTTGATGCT
CAAGACTCAGCGGGGCGATGTGACCAGCAGTGGTTCACTGGATCATGTGCAAATCGACATGGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

85.326

100

0.853
  pilU Pseudomonas stutzeri DSM 10701

57.143

95.109

0.543
  pilU Acinetobacter baylyi ADP1

54.416

95.38

0.519
  pilT Acinetobacter baylyi ADP1

41.642

92.663

0.386
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.692

88.315

0.386
  pilT Legionella pneumophila strain ERS1305867

41.593

92.12

0.383
  pilT Legionella pneumophila strain Lp02

41.593

92.12

0.383
  pilT Acinetobacter baumannii D1279779

40.708

92.12

0.375
  pilT Acinetobacter baumannii strain A118

40.708

92.12

0.375
  pilT Acinetobacter nosocomialis M2

40.708

92.12

0.375
  pilT Pseudomonas aeruginosa PAK

41.194

91.033

0.375
  pilT Pseudomonas stutzeri DSM 10701

39.701

91.033

0.361

Multiple sequence alignment