Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CV024_RS03495 Genome accession   NZ_CP156745
Coordinates   728190..728609 (-) Length   139 a.a.
NCBI ID   WP_047459095.1    Uniprot ID   A0AAX2LK83
Organism   Vibrio fluvialis strain QY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 723190..733609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV024_RS03475 (CV024_03475) coaE 723698..724306 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  CV024_RS03480 (CV024_03480) pilD 724309..725178 (-) 870 WP_047459100.1 A24 family peptidase Machinery gene
  CV024_RS03485 (CV024_03485) pilC 725230..726456 (-) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  CV024_RS03490 (CV024_03490) pilB 726492..728180 (-) 1689 WP_100268243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CV024_RS03495 (CV024_03495) pilA 728190..728609 (-) 420 WP_047459095.1 pilin Machinery gene
  CV024_RS03500 (CV024_03500) nadC 728841..729728 (-) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CV024_RS03505 (CV024_03505) ampD 729881..730432 (+) 552 WP_020331981.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CV024_RS03510 (CV024_03510) fldB 730437..730958 (-) 522 WP_020331982.1 flavodoxin FldB -
  CV024_RS03515 (CV024_03515) xerD 731099..732007 (+) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  CV024_RS03520 (CV024_03520) dsbC 732024..732788 (+) 765 WP_020331984.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14270.22 Da        Isoelectric Point: 8.4642

>NTDB_id=1002453 CV024_RS03495 WP_047459095.1 728190..728609(-) (pilA) [Vibrio fluvialis strain QY27]
MNANQRKQQQGFTLIELMIVVAIIGVLSAIAVPAYKDYVTKSELASGFATIKSVLTPAELYVQENGKISSGVSTITSLGV
SSAANSLGTLSISADNEITFSHTDGSAKGAKFVYTRTTGGWSCAYTGPAAVSAAAPKGC

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1002453 CV024_RS03495 WP_047459095.1 728190..728609(-) (pilA) [Vibrio fluvialis strain QY27]
ATGAACGCGAATCAACGCAAACAACAGCAGGGCTTTACCCTGATCGAACTGATGATCGTGGTCGCCATCATCGGAGTTCT
GTCGGCGATTGCCGTACCCGCTTATAAGGATTACGTAACCAAGAGTGAGTTAGCTTCTGGCTTTGCCACAATTAAATCCG
TGCTAACACCAGCAGAGTTATATGTCCAAGAAAATGGAAAGATTAGTAGTGGAGTAAGTACCATCACATCGCTGGGTGTT
TCCTCTGCCGCTAATAGCTTAGGCACACTAAGTATCTCAGCAGACAATGAAATCACCTTTTCTCACACTGATGGTTCAGC
AAAGGGCGCGAAGTTCGTATACACAAGAACAACTGGTGGCTGGTCATGTGCGTACACCGGCCCAGCAGCCGTGAGCGCAG
CAGCACCGAAAGGTTGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

50.746

96.403

0.489
  pilA Acinetobacter baumannii strain A118

41.83

100

0.46
  pilA Pseudomonas aeruginosa PAK

40.667

100

0.439
  pilA Vibrio cholerae C6706

38.312

100

0.424
  pilA Vibrio cholerae strain A1552

38.312

100

0.424
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.312

100

0.424
  pilA2 Legionella pneumophila str. Paris

37.857

100

0.381
  pilA2 Legionella pneumophila strain ERS1305867

37.143

100

0.374
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.31

92.806

0.374

Multiple sequence alignment