Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   EL199_RS10225 Genome accession   NZ_LR134365
Coordinates   2100887..2102185 (-) Length   432 a.a.
NCBI ID   WP_004141680.1    Uniprot ID   C8NB70
Organism   Cardiobacterium hominis strain NCTC10426     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2095887..2107185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL199_RS10210 (NCTC10426_01991) - 2097092..2098369 (-) 1278 WP_004141683.1 type II toxin-antitoxin system HipA family toxin -
  EL199_RS10215 (NCTC10426_01992) - 2098356..2098643 (-) 288 WP_004141682.1 helix-turn-helix domain-containing protein -
  EL199_RS10220 (NCTC10426_01993) parE 2098800..2100719 (-) 1920 WP_040355505.1 DNA topoisomerase IV subunit B -
  EL199_RS10225 (NCTC10426_01994) pilU 2100887..2102185 (-) 1299 WP_004141680.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  EL199_RS10230 (NCTC10426_01995) pilT 2102201..2103262 (-) 1062 WP_004141679.1 type IV pilus twitching motility protein PilT Machinery gene
  EL199_RS10235 (NCTC10426_01996) proC 2103413..2104255 (+) 843 WP_004141675.1 pyrroline-5-carboxylate reductase -
  EL199_RS10240 (NCTC10426_01997) - 2104294..2104809 (+) 516 WP_004141674.1 YggT family protein -
  EL199_RS10245 (NCTC10426_01998) - 2104813..2105106 (+) 294 WP_004141673.1 DUF167 family protein -
  EL199_RS10250 (NCTC10426_01999) mnmG 2105103..2106980 (+) 1878 WP_004141672.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49370.62 Da        Isoelectric Point: 6.3297

>NTDB_id=1002453 EL199_RS10225 WP_004141680.1 2100887..2102185(-) (pilU) [Cardiobacterium hominis strain NCTC10426]
MEHATLDTALDLTPISRDEIAQKKTSMAREELRPMFDKLVAHAAEKKASDIFINSENYISIKTDGQLNYTKKYMEEGDVY
NLIEVISRPEAFREFLRNHELNMMVEVPNVTYLRVNVYLQKNVPGLVLRLIPAMIPKLDDLELPVPDILKKISMVKRGLV
IVIGATGNGKSTTLASMIDHRNENSSNHIITVEDPIEFVHKPKKSVVIQREVGIDTASYGTALKNSLRQAPDVILIGEIR
DVDTMQYAMHFAETGHLCLATLHATNSVQALERIYNFFPREQRHQIQPELAENLHCLITQRLLPRSDRPGRIVAMEMMMN
TPYISHLISEGDLGSIPEAMERGNPAEGVFTFDHSIFELYESGVISFQDAINYVESENNFRIRIRAQSNRRLPPEMQSRG
EIFSVKSDDALSRELMRKDREERERLRKEGKL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1002453 EL199_RS10225 WP_004141680.1 2100887..2102185(-) (pilU) [Cardiobacterium hominis strain NCTC10426]
ATGGAACACGCAACCCTTGATACCGCCCTTGATCTCACCCCGATCAGCCGCGACGAAATCGCCCAGAAAAAAACCAGCAT
GGCGCGCGAAGAACTGCGGCCGATGTTCGACAAACTGGTGGCGCACGCCGCCGAGAAAAAAGCGTCGGATATCTTCATCA
ACTCCGAGAACTACATCAGCATCAAAACCGACGGCCAGCTCAACTACACCAAAAAATACATGGAAGAGGGCGACGTCTAT
AACCTGATTGAGGTCATCTCGCGTCCGGAAGCCTTCCGCGAATTCCTGCGCAACCACGAACTGAACATGATGGTGGAAGT
GCCGAACGTTACCTACCTGCGGGTCAACGTTTACCTGCAGAAAAACGTTCCCGGCCTCGTGCTACGTCTGATTCCGGCGA
TGATTCCGAAACTGGACGACCTCGAATTGCCGGTACCGGACATTCTGAAAAAAATCTCCATGGTCAAACGCGGTCTGGTT
ATCGTCATCGGGGCGACCGGTAACGGTAAATCCACCACGCTGGCTTCGATGATCGATCACCGCAACGAAAACTCCAGCAA
CCACATCATCACCGTCGAAGACCCGATCGAATTCGTGCACAAGCCGAAAAAATCCGTGGTCATCCAGCGTGAAGTCGGCA
TTGACACCGCCTCCTACGGCACCGCGTTGAAAAACTCACTGCGTCAGGCGCCGGACGTCATCCTCATCGGGGAAATCCGG
GATGTGGATACCATGCAATACGCGATGCACTTCGCCGAAACCGGTCACCTGTGTCTCGCGACCCTGCACGCCACCAACTC
CGTACAAGCGCTGGAGCGGATTTACAACTTCTTCCCGCGTGAGCAGCGCCACCAAATCCAGCCGGAACTTGCAGAAAACC
TGCACTGCCTCATCACCCAGCGCCTCCTTCCGCGCTCTGACCGCCCCGGCCGGATAGTCGCCATGGAAATGATGATGAAC
ACCCCGTACATCAGCCACCTCATCTCCGAAGGCGACCTCGGCTCCATTCCCGAGGCGATGGAACGCGGCAACCCGGCGGA
AGGCGTCTTTACCTTCGACCACAGCATCTTCGAGCTGTACGAATCGGGCGTTATCTCCTTCCAGGACGCCATCAACTACG
TCGAATCCGAAAACAACTTCCGCATCCGCATCCGTGCCCAGTCCAACCGCCGTCTGCCGCCGGAAATGCAATCACGCGGC
GAAATCTTCAGCGTCAAAAGCGACGACGCGCTCTCGCGCGAACTCATGCGCAAAGACCGCGAAGAACGCGAACGCCTACG
CAAAGAAGGCAAGCTGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(39-289)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB C8NB70

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

45.082

84.722

0.382
  pilU Pseudomonas stutzeri DSM 10701

45.48

81.944

0.373
  pilU Vibrio cholerae strain A1552

44.258

82.639

0.366

Multiple sequence alignment