Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CV024_RS03485 Genome accession   NZ_CP156745
Coordinates   725230..726456 (-) Length   408 a.a.
NCBI ID   WP_020332199.1    Uniprot ID   S7J7L5
Organism   Vibrio fluvialis strain QY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 720230..731456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV024_RS03460 (CV024_03460) - 721432..722638 (+) 1207 Protein_635 IS3 family transposase -
  CV024_RS03465 (CV024_03465) yacG 722673..722870 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  CV024_RS03470 (CV024_03470) zapD 722925..723665 (-) 741 WP_100268242.1 cell division protein ZapD -
  CV024_RS03475 (CV024_03475) coaE 723698..724306 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  CV024_RS03480 (CV024_03480) pilD 724309..725178 (-) 870 WP_047459100.1 A24 family peptidase Machinery gene
  CV024_RS03485 (CV024_03485) pilC 725230..726456 (-) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  CV024_RS03490 (CV024_03490) pilB 726492..728180 (-) 1689 WP_100268243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CV024_RS03495 (CV024_03495) pilA 728190..728609 (-) 420 WP_047459095.1 pilin Machinery gene
  CV024_RS03500 (CV024_03500) nadC 728841..729728 (-) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CV024_RS03505 (CV024_03505) ampD 729881..730432 (+) 552 WP_020331981.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CV024_RS03510 (CV024_03510) fldB 730437..730958 (-) 522 WP_020331982.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44670.91 Da        Isoelectric Point: 10.5318

>NTDB_id=1002451 CV024_RS03485 WP_020332199.1 725230..726456(-) (pilC) [Vibrio fluvialis strain QY27]
MKPTKGPELKNYRWKGVNSTGKKVAGQTLAISEIEVRDKLKEQHIQVKKIKKGSVSVLTRLTNRVKTKDITILTRQLATM
LTTGVPIVQALKLVGDNHRKAEMKSILAQITKGVEAGTPISKAMRTASTHFDALYVDLIQTGEQSGNLAEVFERLATYRE
KSEQLRAKVIKALIYPSMVILVALGVSYLMLTMVIPEFESMFKGFGAELPWFTQQILKLSHGVQAYSAGAFIASLGLVFG
IKAARKKSFVVRLKTSQLSLRFPIIGGVLAKAAIAKFSRTLATSFSAGIPILASLKTTAKTAGNVHFETAIQDVHRHTAA
GMPMYIAMRNTQAFPEMVLQMVMIGEESGKLDDMLNKVATIYEFEVDNTVDNLGKILEPLIIVFLGIVVGGLVVAMYLPI
FNLMSVLG

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1002451 CV024_RS03485 WP_020332199.1 725230..726456(-) (pilC) [Vibrio fluvialis strain QY27]
ATGAAACCGACCAAAGGCCCTGAGTTAAAAAACTATCGCTGGAAAGGCGTCAACAGCACTGGCAAGAAAGTCGCGGGACA
GACACTGGCTATCAGTGAAATAGAAGTCCGCGACAAACTGAAAGAACAGCACATTCAGGTTAAGAAGATCAAAAAAGGCA
GCGTGTCAGTTCTGACTCGTCTCACCAATCGGGTCAAAACTAAAGACATCACCATCCTAACCCGCCAGCTTGCCACCATG
CTGACCACCGGAGTGCCAATTGTGCAGGCACTTAAATTGGTCGGCGATAATCATCGTAAAGCCGAGATGAAATCGATTCT
GGCGCAAATTACCAAAGGCGTCGAAGCCGGTACGCCGATTTCCAAAGCGATGCGCACCGCCAGCACTCACTTTGACGCGT
TGTACGTCGACTTAATTCAGACTGGCGAGCAGTCCGGCAATCTTGCCGAAGTCTTTGAACGCCTTGCAACCTATCGGGAA
AAAAGCGAACAACTGCGCGCGAAAGTAATCAAAGCGCTGATCTATCCCAGCATGGTCATTCTGGTGGCACTCGGCGTCTC
CTATTTGATGCTGACGATGGTCATTCCAGAGTTCGAAAGTATGTTCAAGGGTTTTGGGGCTGAACTGCCTTGGTTTACTC
AGCAGATCCTCAAGTTATCGCATGGGGTACAAGCCTACAGTGCGGGGGCATTCATCGCGTCATTGGGCTTGGTGTTTGGC
ATCAAAGCAGCGCGGAAAAAATCCTTCGTCGTCCGTCTTAAAACCAGCCAGCTCAGCCTGCGTTTTCCCATCATTGGCGG
TGTGCTTGCCAAAGCCGCAATTGCCAAATTCAGCCGCACTCTGGCGACCAGCTTCAGTGCGGGAATTCCGATACTCGCCA
GCCTCAAAACCACCGCTAAAACGGCAGGTAACGTGCATTTTGAAACCGCCATTCAGGATGTTCACCGCCATACGGCAGCA
GGGATGCCGATGTATATTGCAATGCGCAACACACAAGCCTTTCCGGAAATGGTGTTGCAAATGGTGATGATCGGCGAGGA
GTCCGGCAAGCTCGACGACATGCTCAATAAAGTCGCCACCATCTATGAGTTTGAAGTCGACAACACCGTCGACAATCTGG
GCAAAATTCTTGAACCGCTGATTATCGTATTTTTGGGCATTGTGGTCGGTGGATTGGTGGTGGCGATGTACCTGCCAATC
TTTAACTTAATGAGTGTATTAGGTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(277-399)

Type II secretion system (T2SS), protein F (T2SSF)

(74-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7J7L5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio cholerae strain A1552

87.01

100

0.87
  pilC Vibrio campbellii strain DS40M4

73.105

100

0.733
  pilC Acinetobacter baumannii D1279779

41.667

100

0.417
  pilC Legionella pneumophila strain ERS1305867

41.353

97.794

0.404
  pilC Acinetobacter baylyi ADP1

41.31

97.304

0.402
  pilC Pseudomonas stutzeri DSM 10701

40.152

97.059

0.39
  pilG Neisseria gonorrhoeae MS11

39.599

97.794

0.387
  pilG Neisseria meningitidis 44/76-A

39.098

97.794

0.382

Multiple sequence alignment