Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CV024_RS03490 Genome accession   NZ_CP156745
Coordinates   726492..728180 (-) Length   562 a.a.
NCBI ID   WP_100268243.1    Uniprot ID   -
Organism   Vibrio fluvialis strain QY27     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 721492..733180
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV024_RS03465 (CV024_03465) yacG 722673..722870 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  CV024_RS03470 (CV024_03470) zapD 722925..723665 (-) 741 WP_100268242.1 cell division protein ZapD -
  CV024_RS03475 (CV024_03475) coaE 723698..724306 (-) 609 WP_061056140.1 dephospho-CoA kinase -
  CV024_RS03480 (CV024_03480) pilD 724309..725178 (-) 870 WP_047459100.1 A24 family peptidase Machinery gene
  CV024_RS03485 (CV024_03485) pilC 725230..726456 (-) 1227 WP_020332199.1 type II secretion system F family protein Machinery gene
  CV024_RS03490 (CV024_03490) pilB 726492..728180 (-) 1689 WP_100268243.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CV024_RS03495 (CV024_03495) pilA 728190..728609 (-) 420 WP_047459095.1 pilin Machinery gene
  CV024_RS03500 (CV024_03500) nadC 728841..729728 (-) 888 WP_061056143.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CV024_RS03505 (CV024_03505) ampD 729881..730432 (+) 552 WP_020331981.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CV024_RS03510 (CV024_03510) fldB 730437..730958 (-) 522 WP_020331982.1 flavodoxin FldB -
  CV024_RS03515 (CV024_03515) xerD 731099..732007 (+) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  CV024_RS03520 (CV024_03520) dsbC 732024..732788 (+) 765 WP_020331984.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 61970.87 Da        Isoelectric Point: 6.1969

>NTDB_id=1002452 CV024_RS03490 WP_100268243.1 726492..728180(-) (pilB) [Vibrio fluvialis strain QY27]
MLTNLLAILRQADLLSPTQEQAVMDHIHASGTNVPEALIDLAIFNHHDLVEHISQIFGLPNTDLTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQVLTLAVSDPTHLQVEDEFRFATGLQVELVLADHKALQAAIRRLYGRAITSHGTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAESVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNSALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=1002452 CV024_RS03490 WP_100268243.1 726492..728180(-) (pilB) [Vibrio fluvialis strain QY27]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGCCCTACGCAGGAACAGGCCGTAATGGATCATAT
TCACGCTTCCGGAACCAATGTTCCGGAGGCGTTGATCGATTTGGCGATCTTTAATCATCACGATCTGGTCGAACATATCA
GCCAGATCTTTGGTCTGCCGAATACGGATCTGACCCGTTTTGACTTCGCGCCACTGTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCTCTGCCCCTCAATAGCAACGGTCAGGTACTGACTCTTGCGGTATCCGATCCGACTCACCT
ACAAGTTGAAGACGAGTTTCGTTTTGCGACCGGGTTACAAGTAGAACTGGTATTAGCTGATCATAAAGCACTGCAAGCCG
CTATTCGCCGCTTGTATGGCCGAGCCATCACGTCACATGGCACGCAGGGCAAGGAGATCAGCCAAGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTTATTAA
CCAGATCCTGCTGGATGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAGCCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCCAGCCATTTAAGCCGTCGACTGGCCGCGCGGATTAAGATT
TTGTCGAAGCTCGATATCGCCGAGCGACGCTTACCCCAGGACGGCCGCATCAAATTACGCCTCAATGCGGACACGGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTTTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGCATTCTCAACACCGACGAAGTGAA
TATTTCTACCGCCGAAGACCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCGTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTGGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAAGCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTTAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GACGCCTTTGCCCTCACTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAG
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACTCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTC
TATTTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.206

100

0.822
  pilB Vibrio campbellii strain DS40M4

72.242

100

0.722
  pilB Vibrio parahaemolyticus RIMD 2210633

71.53

100

0.715
  pilB Legionella pneumophila strain ERS1305867

52.611

91.993

0.484
  pilB Acinetobacter baumannii D1279779

50.289

92.349

0.464
  pilB Acinetobacter baylyi ADP1

48.956

93.772

0.459
  pilF Neisseria gonorrhoeae MS11

44.858

100

0.45
  pilF Thermus thermophilus HB27

37.544

100

0.375
  pilB/pilB1 Synechocystis sp. PCC 6803

37.455

99.288

0.372

Multiple sequence alignment