Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU77_RS17485 Genome accession   NZ_CP155972
Coordinates   4081907..4083040 (+) Length   377 a.a.
NCBI ID   WP_012439300.1    Uniprot ID   Q93S76
Organism   Xanthomonas campestris pv. raphani strain bglFP 6790     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4076907..4088040
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU77_RS17460 (ABFU77_17430) - 4077083..4078282 (-) 1200 WP_323536794.1 cystathionine gamma-synthase -
  ABFU77_RS17465 (ABFU77_17435) - 4078376..4079746 (-) 1371 WP_014506550.1 pyridoxal-phosphate dependent enzyme -
  ABFU77_RS17470 (ABFU77_17440) - 4079978..4080196 (-) 219 WP_019237985.1 YdcH family protein -
  ABFU77_RS17475 (ABFU77_17445) - 4080538..4081428 (-) 891 WP_323536792.1 hypothetical protein -
  ABFU77_RS17480 (ABFU77_17450) - 4081425..4081808 (-) 384 WP_323536791.1 DUF4398 domain-containing protein -
  ABFU77_RS17485 (ABFU77_17455) pilU 4081907..4083040 (+) 1134 WP_012439300.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU77_RS17490 (ABFU77_17460) maiA 4083560..4084222 (-) 663 WP_323536790.1 maleylacetoacetate isomerase -
  ABFU77_RS17495 (ABFU77_17465) - 4084266..4085255 (-) 990 WP_323536789.1 fumarylacetoacetate hydrolase family protein -
  ABFU77_RS17500 (ABFU77_17470) - 4085635..4087023 (+) 1389 WP_011035834.1 DEAD/DEAH box helicase -
  ABFU77_RS17505 (ABFU77_17475) queD 4087139..4087492 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ABFU77_RS17510 (ABFU77_17480) - 4087682..4088017 (+) 336 WP_014506541.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41799.10 Da        Isoelectric Point: 6.1401

>NTDB_id=1000525 ABFU77_RS17485 WP_012439300.1 4081907..4083040(+) (pilU) [Xanthomonas campestris pv. raphani strain bglFP 6790]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1000525 ABFU77_RS17485 WP_012439300.1 4081907..4083040(+) (pilU) [Xanthomonas campestris pv. raphani strain bglFP 6790]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACGCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTAGGCCTGGACACGCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATT
CTCAACTTCTTCCCCGAAAGTGCGCACAAGAACGTGCTGATGAACCTGGCACTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCAATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q93S76

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.604

89.655

0.382
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.018

88.594

0.363

Multiple sequence alignment