Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU65_RS18000 Genome accession   NZ_CP155964
Coordinates   4134281..4135414 (+) Length   377 a.a.
NCBI ID   WP_014506546.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BR25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4129281..4140414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU65_RS17975 (ABFU65_17910) - 4129457..4130656 (-) 1200 WP_116921851.1 cystathionine gamma-synthase -
  ABFU65_RS17980 (ABFU65_17915) - 4130750..4132120 (-) 1371 WP_323549954.1 pyridoxal-phosphate dependent enzyme -
  ABFU65_RS17985 (ABFU65_17920) - 4132352..4132570 (-) 219 WP_014506549.1 YdcH family protein -
  ABFU65_RS17990 (ABFU65_17925) - 4132912..4133802 (-) 891 WP_228427832.1 hypothetical protein -
  ABFU65_RS17995 (ABFU65_17930) - 4133799..4134182 (-) 384 WP_011035838.1 DUF4398 domain-containing protein -
  ABFU65_RS18000 (ABFU65_17935) pilU 4134281..4135414 (+) 1134 WP_014506546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU65_RS18005 (ABFU65_17940) maiA 4135936..4136598 (-) 663 WP_014506545.1 maleylacetoacetate isomerase -
  ABFU65_RS18010 (ABFU65_17945) - 4136642..4137631 (-) 990 WP_323547575.1 fumarylacetoacetate hydrolase family protein -
  ABFU65_RS18015 (ABFU65_17950) - 4138011..4139399 (+) 1389 WP_011035834.1 DEAD/DEAH box helicase -
  ABFU65_RS18020 (ABFU65_17955) queD 4139515..4139868 (+) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ABFU65_RS18025 (ABFU65_17960) - 4140058..4140393 (+) 336 WP_011035833.1 phasin family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41727.04 Da        Isoelectric Point: 6.2909

>NTDB_id=1000383 ABFU65_RS18000 WP_014506546.1 4134281..4135414(+) (pilU) [Xanthomonas campestris pv. raphani strain BR25]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQGEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1000383 ABFU65_RS18000 WP_014506546.1 4134281..4135414(+) (pilU) [Xanthomonas campestris pv. raphani strain BR25]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCAATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTAGGCCTGGACACGCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCATAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGGGGAAGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGGGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366

Multiple sequence alignment