Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ABFU65_RS05040 Genome accession   NZ_CP155964
Coordinates   1196576..1196995 (+) Length   139 a.a.
NCBI ID   WP_323549479.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BR25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1193455..1212566 1196576..1196995 within 0


Gene organization within MGE regions


Location: 1193455..1212566
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU65_RS05025 (ABFU65_05025) coaE 1193455..1194078 (-) 624 WP_323549481.1 dephospho-CoA kinase -
  ABFU65_RS05030 (ABFU65_05030) - 1194092..1194955 (-) 864 WP_011038216.1 A24 family peptidase -
  ABFU65_RS05035 (ABFU65_05035) pilC 1194962..1196221 (-) 1260 WP_323549480.1 type II secretion system F family protein Machinery gene
  ABFU65_RS05040 (ABFU65_05040) pilA2 1196576..1196995 (+) 420 WP_323549479.1 pilin Machinery gene
  ABFU65_RS05045 (ABFU65_05045) - 1197077..1198978 (+) 1902 WP_323549478.1 hypothetical protein -
  ABFU65_RS05050 (ABFU65_05050) - 1198975..1199433 (+) 459 WP_323549477.1 GtrA family protein -
  ABFU65_RS05055 (ABFU65_05055) - 1199439..1200983 (+) 1545 WP_323552249.1 NAD(P)/FAD-dependent oxidoreductase -
  ABFU65_RS05060 (ABFU65_05060) galE 1201079..1202056 (+) 978 WP_323550507.1 UDP-glucose 4-epimerase GalE -
  ABFU65_RS05065 (ABFU65_05065) pilB 1202199..1203905 (+) 1707 WP_323550509.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFU65_RS05070 (ABFU65_05070) - 1204088..1204717 (-) 630 WP_407472217.1 toxin -
  ABFU65_RS05075 (ABFU65_05075) - 1204816..1205614 (+) 799 WP_087941972.1 IS5 family transposase -
  ABFU65_RS05080 (ABFU65_05080) - 1205676..1206038 (-) 363 WP_148263072.1 hypothetical protein -
  ABFU65_RS05085 (ABFU65_05085) pilR 1206230..1207624 (-) 1395 WP_043921909.1 sigma-54 dependent transcriptional regulator Regulator
  ABFU65_RS05090 (ABFU65_05090) - 1207833..1209443 (-) 1611 WP_014508702.1 PAS domain-containing sensor histidine kinase -
  ABFU65_RS05095 (ABFU65_05095) sucC 1209677..1210846 (+) 1170 WP_011038209.1 ADP-forming succinate--CoA ligase subunit beta -
  ABFU65_RS05100 (ABFU65_05100) sucD 1210871..1211746 (+) 876 WP_011038208.1 succinate--CoA ligase subunit alpha -
  ABFU65_RS05105 (ABFU65_05105) - 1211850..1212260 (+) 411 WP_029628876.1 CopG family ribbon-helix-helix protein -
  ABFU65_RS05110 (ABFU65_05110) - 1212264..1212566 (+) 303 WP_323475834.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 139 a.a.        Molecular weight: 14202.21 Da        Isoelectric Point: 9.0169

>NTDB_id=1000370 ABFU65_RS05040 WP_323549479.1 1196576..1196995(+) (pilA2) [Xanthomonas campestris pv. raphani strain BR25]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYLVKSRVSEVVLAASSARTTVSEAAASDTGANMPATVNIESQSSKYVS
GVTYAQSGANGVITATAKDLGGSTPTGTITLTGTKGANGQVNWVCSGSIAPKYRPSTCQ

Nucleotide


Download         Length: 420 bp        

>NTDB_id=1000370 ABFU65_RS05040 WP_323549479.1 1196576..1196995(+) (pilA2) [Xanthomonas campestris pv. raphani strain BR25]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTTGTGGCGATCATTGCCATCCTGGCGGCAATCGCTCT
GCCCGCGTACCAGGACTATCTGGTCAAGTCGCGCGTGTCGGAAGTGGTGCTGGCTGCCTCGTCGGCTCGTACTACTGTGT
CCGAGGCCGCCGCTTCTGACACCGGCGCAAACATGCCTGCGACTGTTAATATCGAGTCGCAGTCTTCCAAATACGTATCG
GGCGTTACGTATGCACAGAGCGGCGCTAATGGCGTGATCACCGCAACTGCCAAGGATCTGGGCGGTAGCACCCCCACTGG
TACGATCACTCTTACCGGCACCAAGGGTGCTAACGGCCAGGTCAATTGGGTGTGCAGTGGCTCCATCGCTCCCAAGTACC
GCCCGTCTACCTGCCAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-139)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

48.951

100

0.504
  pilA2 Legionella pneumophila str. Paris

48.951

100

0.504
  comP Acinetobacter baylyi ADP1

44.079

100

0.482
  pilA Ralstonia pseudosolanacearum GMI1000

37.725

100

0.453
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.239

100

0.453
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.278

100

0.417
  pilE Neisseria gonorrhoeae MS11

35.366

100

0.417
  pilE Neisseria gonorrhoeae strain FA1090

35.404

100

0.41
  pilA/pilA1 Eikenella corrodens VA1

37.748

100

0.41
  pilA Pseudomonas aeruginosa PAK

35.294

100

0.388
  pilA Acinetobacter baumannii strain A118

36.62

100

0.374
  pilA Vibrio parahaemolyticus RIMD 2210633

36.429

100

0.367

Multiple sequence alignment