Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU20_RS03450 Genome accession   NZ_CP155963
Coordinates   773485..774618 (-) Length   377 a.a.
NCBI ID   WP_014506546.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain BR66     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 768485..779618
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU20_RS03425 (ABFU20_03430) - 768508..768843 (-) 336 WP_014506541.1 phasin family protein -
  ABFU20_RS03430 (ABFU20_03435) queD 769033..769386 (-) 354 WP_008576903.1 6-carboxytetrahydropterin synthase QueD -
  ABFU20_RS03435 (ABFU20_03440) - 769502..770890 (-) 1389 WP_014506542.1 DEAD/DEAH box helicase -
  ABFU20_RS03440 (ABFU20_03445) - 771270..772259 (+) 990 WP_014506544.1 fumarylacetoacetate hydrolase family protein -
  ABFU20_RS03445 (ABFU20_03450) maiA 772303..772965 (+) 663 WP_014506545.1 maleylacetoacetate isomerase -
  ABFU20_RS03450 (ABFU20_03455) pilU 773485..774618 (-) 1134 WP_014506546.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU20_RS03455 (ABFU20_03460) - 774717..775100 (+) 384 WP_011035838.1 DUF4398 domain-containing protein -
  ABFU20_RS03460 (ABFU20_03465) - 775097..775987 (+) 891 WP_014506548.1 membrane protein -
  ABFU20_RS03465 (ABFU20_03470) - 776329..776547 (+) 219 WP_014506549.1 YdcH family protein -
  ABFU20_RS03470 (ABFU20_03475) - 776779..778149 (+) 1371 WP_014506550.1 pyridoxal-phosphate dependent enzyme -
  ABFU20_RS03475 (ABFU20_03480) - 778243..779442 (+) 1200 WP_014506551.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41727.04 Da        Isoelectric Point: 6.2909

>NTDB_id=1000353 ABFU20_RS03450 WP_014506546.1 773485..774618(-) (pilU) [Xanthomonas campestris pv. raphani strain BR66]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALSDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQGEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1000353 ABFU20_RS03450 WP_014506546.1 773485..774618(-) (pilU) [Xanthomonas campestris pv. raphani strain BR66]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCTCTGTCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCCTCAATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTAGGCCTGGACACGCATGCTTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCCGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCATAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGGGGAAGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGGGAGCACGATCCGTATGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(76-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.308

89.655

0.379
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.124

89.655

0.369
  pilT Acinetobacter nosocomialis M2

41.124

89.655

0.369
  pilT Acinetobacter baumannii strain A118

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.588

90.186

0.366
  pilT Pseudomonas stutzeri DSM 10701

40.828

89.655

0.366

Multiple sequence alignment