Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   ABFT91_RS05215 Genome accession   NZ_CP155960
Coordinates   1237772..1238197 (+) Length   141 a.a.
NCBI ID   WP_323539945.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain NBC567     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1232772..1243197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT91_RS05185 (ABFT91_05180) - 1232868..1233296 (-) 429 WP_345785850.1 GNAT family N-acetyltransferase -
  ABFT91_RS05190 (ABFT91_05185) - 1233356..1233646 (-) 291 WP_014508712.1 DUF1778 domain-containing protein -
  ABFT91_RS05195 (ABFT91_05190) - 1233721..1234122 (-) 402 WP_323530793.1 SymE family type I addiction module toxin -
  ABFT91_RS05200 (ABFT91_05195) coaE 1234654..1235277 (-) 624 WP_116670565.1 dephospho-CoA kinase -
  ABFT91_RS05205 (ABFT91_05200) - 1235291..1236154 (-) 864 WP_259177416.1 A24 family peptidase -
  ABFT91_RS05210 (ABFT91_05205) pilC 1236161..1237420 (-) 1260 WP_323539944.1 type II secretion system F family protein Machinery gene
  ABFT91_RS05215 (ABFT91_05210) pilA2 1237772..1238197 (+) 426 WP_323539945.1 pilin Machinery gene
  ABFT91_RS05220 (ABFT91_05215) - 1238332..1239921 (+) 1590 WP_323539946.1 phosphoethanolamine transferase -
  ABFT91_RS05225 (ABFT91_05220) - 1239935..1240561 (+) 627 WP_323539947.1 hypothetical protein -
  ABFT91_RS05230 (ABFT91_05225) - 1240531..1242057 (+) 1527 WP_323539948.1 hypothetical protein -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14443.67 Da        Isoelectric Point: 9.0253

>NTDB_id=1000300 ABFT91_RS05215 WP_323539945.1 1237772..1238197(+) (pilA2) [Xanthomonas campestris pv. raphani strain NBC567]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQDYTTRAKLSEALTMSAPAKLAVTETASSLGGLTNVTAANSGYAFPGATK
YVSGITITSATGQVAVTSTVPNATGTILLTPTDVGGGQLRWTCSSAINVKYLPAECRNSGT

Nucleotide


Download         Length: 426 bp        

>NTDB_id=1000300 ABFT91_RS05215 WP_323539945.1 1237772..1238197(+) (pilA2) [Xanthomonas campestris pv. raphani strain NBC567]
ATGAAGAAGCAGCAAGGCTTTACACTTATCGAACTGATGATCGTGGTCGCGATCATCGCCATCTTGGCCGCCATCGCGCT
GCCGGCTTATCAGGACTACACCACTCGCGCCAAACTCAGTGAAGCGCTGACGATGTCGGCGCCTGCCAAGCTTGCGGTAA
CGGAGACTGCATCGTCGCTCGGCGGTCTTACTAATGTTACTGCTGCGAATTCGGGCTACGCATTCCCGGGTGCCACGAAG
TATGTCAGCGGCATCACTATCACCAGTGCTACGGGCCAAGTCGCTGTAACCTCCACCGTGCCGAACGCAACCGGTACCAT
CCTGCTGACCCCGACTGATGTCGGTGGTGGCCAGCTGAGGTGGACCTGCTCTTCCGCCATCAACGTCAAGTACCTGCCGG
CGGAGTGCCGTAATTCGGGCACCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

52.941

96.454

0.511
  pilA2 Legionella pneumophila str. Paris

52.941

96.454

0.511
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.297

100

0.489
  comP Acinetobacter baylyi ADP1

44.898

100

0.468
  pilA Ralstonia pseudosolanacearum GMI1000

39.634

100

0.461
  pilA/pilA1 Eikenella corrodens VA1

40.667

100

0.433
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.478

97.872

0.426
  pilE Neisseria gonorrhoeae MS11

36.646

100

0.418
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.336

97.163

0.411
  pilE Neisseria gonorrhoeae strain FA1090

36.709

100

0.411
  pilA Acinetobacter baumannii strain A118

38.194

100

0.39
  pilA Pseudomonas aeruginosa PAK

35.065

100

0.383

Multiple sequence alignment