Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type I Classification (family/domain) bsrE-as-bsrE/-
Location 2069883..2070129 Replicon chromosome
Accession NC_000964
Organism Bacillus subtilis subsp. subtilis str. 168
T1TAdb ID TA04734

Toxin (Protein)


Gene name bsrE Uniprot ID -
Locus tag BSU_18978 Protein ID YP_009513965.1
Coordinates 2069883..2069975 (+) Length 31 a.a.

Antitoxin (RNA)


Gene name as-bsrE
Locus tag -
Coordinates 2069956..2070129 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BSU_18960 (BSU18960) 2065042..2065377 + 336 NP_389777.1 putative bacteriophage protein; putative defective prophage 6 -
BSU_18970 (BSU18970) 2065424..2069029 - 3606 NP_389778.1 putative phage repair NTPase with transmembrane helices; putative defective prophage 6 -
BSU_18978 (BSU18978) 2069883..2069975 + 93 YP_009513965.1 type I toxin (BsrE/AsrE) Toxin
- 2069956..2070129 - 174 - - Antitoxin
BSU_18980 (BSU18980) 2070244..2071086 - 843 NP_389779.1 conserved protein of unknown function; putative defective prophage 6 -
BSU_18990 (BSU18990) 2071286..2071744 - 459 NP_389780.1 antitoxin inhibiting Rnase RttL -
BSU_19000 (BSU19000) 2071754..2073556 - 1803 NP_389781.1 phage toxin ribonuclease; putative defective prophage 6 -
BSU_19010 (BSU19010) 2073658..2074215 - 558 NP_389782.3 putative phage protein; putative defective prophage 6 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 2055868..2100350 44482


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Small toxic protein BsrE-like

(1-24)


Sequences

Toxin        


Download         Length: 31 a.a.        Molecular weight: 3358.16 Da        Isoelectric Point: 4.1840

>T10200 YP_009513965.1 NC_000964:2069883-2069975 [Bacillus subtilis subsp. subtilis str. 168]
MSTFQALMLMLAIGSFIIALLTYIEKIDLP

Download         Length: 93 bp

>T10200 NC_000964:2069883-2069975 [Bacillus subtilis subsp. subtilis str. 168]
ATGTCAACATTCCAGGCATTAATGCTAATGCTTGCAATCGGGTCGTTTATAATCGCCCTATTGACGTATATAGAAAAAAT
AGACCTCCCTTGA

Antitoxin


Download         Length: 174 bp

>AT10200 NC_000964:c2070129-2069956 [Bacillus subtilis subsp. subtilis str. 168]
TTACAATACATTACAGTGCATTTCTTTAATGAGAAATGCATAAAATAAAAAAGACCGAGGTGCTACCAACACCCCGGCTC
TGTACAAAAAGTTGCCCATCAAAGGGCTTGCTCGATGTGGTTCTAGGTAGGCCTATCCCTTCAGGTTCTGAGGCTCAAGG
GAGGTCTATTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10201 Bacillus subtilis subsp. subtilis str. 168

92

86.207

0.793
T10205 Enterococcus faecalis V583

44.444

90

0.4
T10204 Enterococcus faecalis V583

37.037

90

0.333

Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Download structure file

References

(1) Christin Meißner et al. (2016) In Vitro Characterization of the Type I Toxin-Antitoxin System bsrE/SR5 from Bacillus subtilis. The Journal of Biological Chemistry 291(2):560-71. [PubMed:26565032] experimental


experimental experimental literature