Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS01164  |
| T6SS type | Type i4b |
| Strain | Acinetobacter baumannii AbCAN2 |
| Replicon (RefSeq) | chromosome (NZ_CP045428) |
| Location | 1213019..1232429 |
| Function | anti-bacterial |
| Comment | Hcp was detected in the supernatant fraction of ΔvgrGi but not WT AbCAN2 or the complemented strain (vgrGi+), indicating that VgrGi inhibits the T6SS of AbCAN2. Similarly, AbCAN2ΔvgrGi, but not WT or vgrGi+, demonstrated significant levels of interbacterial killing when coincubated with E. coli. |
| References | [1] Lopez J, Ly PM, Feldman MF. et al (2020) The Tip of the VgrG Spike Is Essential to Functional Type VI Secretion System Assembly in Acinetobacter baumannii.. mBio. 11(1). [PudMed:31937641] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| F9K57_RS05800 | 1208900..1209781 | - | 882 | WP_065712157.1 | ABC transporter ATP-binding protein | ||
| F9K57_RS05805 | 1209781..1210725 | - | 945 | WP_039248199.1 | ABC transporter permease | ||
| F9K57_RS05810 | 1210821..1211222 | - | 402 | WP_072618257.1 | hypothetical protein | ||
| F9K57_RS05815 | 1212310..1213002 | + | 693 | WP_000869709.1 | hypothetical protein | ||
| T6CP014819 | TssB | F9K57_RS05820 (tssB) | 1213019..1213522 | + | 504 | WP_001119042.1 | type VI secretion system contractile sheath small subunit |
| T6CP014820 | TssC | F9K57_RS05825 (tssC) | 1213515..1214996 | + | 1482 | WP_001066523.1 | type VI secretion system contractile sheath large subunit |
| T6CP014821 | TssD | F9K57_RS05830 | 1215046..1215549 | + | 504 | WP_000653195.1 | type VI secretion system tube protein Hcp |
| T6CP014822 | TssE | F9K57_RS05835 (tssE) | 1215629..1216105 | + | 477 | WP_001047031.1 | type VI secretion system baseplate subunit TssE |
| T6CP014823 | TssF | F9K57_RS05840 (tssF) | 1216122..1217933 | + | 1812 | WP_000568822.1 | type VI secretion system baseplate subunit TssF |
| T6CP014824 | TssG | F9K57_RS05845 (tssG) | 1217897..1218895 | + | 999 | WP_159141859.1 | type VI secretion system baseplate subunit TssG |
| F9K57_RS05850 | 1218892..1220304 | + | 1413 | WP_159141860.1 | hypothetical protein | ||
| T6CP014825 | TssM | F9K57_RS05855 (tssM) | 1220335..1224159 | + | 3825 | WP_159141861.1 | type VI secretion system membrane subunit TssM |
| F9K57_RS05860 (tagF) | 1224197..1225156 | + | 960 | WP_000557247.1 | type VI secretion system-associated protein TagF | ||
| F9K57_RS05865 | 1225159..1225926 | + | 768 | WP_001072410.1 | OmpA family protein | ||
| F9K57_RS05870 | 1225943..1226206 | + | 264 | WP_000168112.1 | PAAR domain-containing protein | ||
| T6CP014826 | TssH | F9K57_RS05875 (tssH) | 1226425..1229106 | + | 2682 | WP_159141862.1 | type VI secretion system ATPase TssH |
| T6CP014827 | TssA | F9K57_RS05880 (tssA) | 1229130..1230224 | + | 1095 | WP_000020709.1 | type VI secretion system protein TssA |
| T6CP014828 | TssK | F9K57_RS05885 (tssK) | 1230241..1231605 | + | 1365 | WP_000471444.1 | type VI secretion system baseplate subunit TssK |
| T6CP014829 | TssL | F9K57_RS05890 | 1231623..1232429 | + | 807 | WP_000083629.1 | type IVB secretion system protein IcmH/DotU |
| F9K57_RS05895 | 1232442..1233044 | + | 603 | WP_000046446.1 | hypothetical protein | ||
| F9K57_RS05900 | 1233052..1234005 | + | 954 | WP_000972583.1 | M15 family metallopeptidase | ||
| F9K57_RS05905 | 1234008..1234859 | - | 852 | WP_159141863.1 | LysR family transcriptional regulator | ||
| F9K57_RS05910 | 1235032..1235679 | + | 648 | WP_100219255.1 | ankyrin repeat domain-containing protein | ||
| F9K57_RS05915 | 1235695..1236177 | + | 483 | WP_016685894.1 | nucleoside deaminase | ||
| F9K57_RS05920 | 1236177..1237355 | + | 1179 | WP_159141864.1 | cyanate transporter |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|---|---|---|---|---|---|---|---|
| REG0350 |
F9K57_RS04510 (VgrGi) | protein | Global regulator | Acinetobacter baumannii strain AbCAN2 | chromosome (NZ_CP045428) | 925473..928592 (+) | 1797381381 | T6SS structure (↑) |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF01847 |
F9K57_RS05830 | - | - | Acinetobacter baumannii strain AbCAN2 | chromosome (NZ_CP045428) | 1215046..1215549 (+) | 446575849 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|