Detailed information of regulator


Summary

Regulator ID REG0350 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) Bacterial secretion system (map03070)
Related T6SS T6SS01164 experimental (Type i4b)
Strain Acinetobacter baumannii strain AbCAN2
Replicon chromosome
Sequence Protein sequence (1040 a.a.); Nucleotide sequence (3120 bp)
Description Use a transposon mutant library and screened mutants for Hcp secretion result that a VgrG homolog inhibits the T6SS is against the current paradigm of VgrG function.Hcp was detected in the supernatant fraction of ΔvgrGi but not WT AbCAN2 or the complemented strain (vgrGi+), indicating that VgrGi inhibits the T6SS of AbCAN2 and VgrGi-mediated T6SS inhibition is due to a leucine-to-arginine mutation.
Reference
External database links

Locus tag (Gene) F9K57_RS04510 (VgrGi)
Coordinates (Strand) 925473..928592 (+)
NCBI ID 1797381381
RefSeq NZ_CP045428
Uniprot ID -
KEGG ID -
KEGG pathway (determined by BlastKOALA) map03070
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
T6SS structure ↑ STQKNVIAHAQNRLSRFAAQSGLKAVAAQGK leucine-to-arginine mutation is indicated by red color
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 1.6e-58 43..384
DUF2345 PF10106.11 3.2e-40 688..834
T6SS_Vgr PF13296.8 1.4e-37 559..666
Phage_base_V PF04717.14 1.6e-11 483..531
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1039 MFNNISQVLESFGFLSQHRSVYLQFSDASLNSQVFLQRIDGQHYLNQGMTAELICLSTNAHIPLKTFIGVQVAVDQVTDRGSFFRTTGIITGASQGQSDGALTLYKLAISDPTYLWHKRRNSRVFMNKSVKEISEILFQEWQGKSPLFASSLTLDLSGLKQTYDVRPFVMQLNESDYDFLTRLWRSEGISWLIDEAELTVASNMDNIQPQKLRLIDDNNQYQALTRRAIRYHRSSATEQFDSMTSLMADRSLQPTSIFVQRWQPDVLQQTDGAGSVQSKHQHSTNYDNQSLSLEEAWHFSPAWMQDLNGEDGATSASNQQLEKFNQNLSAYYDAQSKQFIAKTTVRDTQVGYWFELNEHPEIDQHESTDKEFLIIGKNYYNQNNLPKDLNQQIQTLLQQSDWQASNTDERQANQLILQRRYIPTTPAYNPQTHSPVAHPQRAKVVGPEGEEIYVDEWGRIKVRFLFTRSDDHSHDGGAGTNNNDTDSAWIDVLTPWAGEGYGARFLPRIGEIVVINFFNGDIDRPFVMGRVHEAQRHPTKFDNKGKLPDTKKLSGIRSKEVSGSGFGQLRFDDTPGQISTQLQSSHGASQLNLGKLSHPKDKAESEDRGEGFELRTDQWGALRAGQGLLVSTHKQDNAKGEHLDAEVAKKQLEGSQTNSKALSDIAKNQKTDEIESLEQLKDFASQIQQQIAKFEKALLLLSSPDGIALSSSEDIHISADAQINQIAGDSINISTQKNVIAHAQNRLSRFAAQSGLKAVAAQGKVEIQAQADALDVLSKLGITISSTDDKVIISSPKEVKITGGSSQITLNGSGIFPKTGGKFQVNAGQHLFMAGASANASAPELPKAKPMQGALELLRSYGGDNFFKQNSYKVIDSLGKQITGKLDGNGFAQVTGIAPGPAKVVFEKDNTSAWLQSSDFKRNYTWAEPVKSVQGLMKNALEAVGQNTMSQLQNNLLSTDKNSFKNLGKNTLANLAGQTVGQIKNQVTNTALNTVSKQLNLNLSADQMKRLGQMATNPSQSLEMLKEQGDLDTFVRSKK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9924 - -


  Protein sequence of regulator: 1040 a.a.    .

>REG0350 NZ_CP045428:925473-928592 [Acinetobacter baumannii]
MFNNISQVLESFGFLSQHRSVYLQFSDASLNSQVFLQRIDGQHYLNQGMTAELICLSTNAHIPLKTFIGVQVAVDQVTDR
GSFFRTTGIITGASQGQSDGALTLYKLAISDPTYLWHKRRNSRVFMNKSVKEISEILFQEWQGKSPLFASSLTLDLSGLK
QTYDVRPFVMQLNESDYDFLTRLWRSEGISWLIDEAELTVASNMDNIQPQKLRLIDDNNQYQALTRRAIRYHRSSATEQF
DSMTSLMADRSLQPTSIFVQRWQPDVLQQTDGAGSVQSKHQHSTNYDNQSLSLEEAWHFSPAWMQDLNGEDGATSASNQQ
LEKFNQNLSAYYDAQSKQFIAKTTVRDTQVGYWFELNEHPEIDQHESTDKEFLIIGKNYYNQNNLPKDLNQQIQTLLQQS
DWQASNTDERQANQLILQRRYIPTTPAYNPQTHSPVAHPQRAKVVGPEGEEIYVDEWGRIKVRFLFTRSDDHSHDGGAGT
NNNDTDSAWIDVLTPWAGEGYGARFLPRIGEIVVINFFNGDIDRPFVMGRVHEAQRHPTKFDNKGKLPDTKKLSGIRSKE
VSGSGFGQLRFDDTPGQISTQLQSSHGASQLNLGKLSHPKDKAESEDRGEGFELRTDQWGALRAGQGLLVSTHKQDNAKG
EHLDAEVAKKQLEGSQTNSKALSDIAKNQKTDEIESLEQLKDFASQIQQQIAKFEKALLLLSSPDGIALSSSEDIHISAD
AQINQIAGDSINISTQKNVIAHAQNRLSRFAAQSGLKAVAAQGKVEIQAQADALDVLSKLGITISSTDDKVIISSPKEVK
ITGGSSQITLNGSGIFPKTGGKFQVNAGQHLFMAGASANASAPELPKAKPMQGALELLRSYGGDNFFKQNSYKVIDSLGK
QITGKLDGNGFAQVTGIAPGPAKVVFEKDNTSAWLQSSDFKRNYTWAEPVKSVQGLMKNALEAVGQNTMSQLQNNLLSTD
KNSFKNLGKNTLANLAGQTVGQIKNQVTNTALNTVSKQLNLNLSADQMKRLGQMATNPSQSLEMLKEQGDLDTFVRSKK*

  Nucleotide sequence of regulator: 3120 bp    .

>REG0350 NZ_CP045428:925473-928592 [Acinetobacter baumannii]
ATGTTTAATAATATTTCTCAGGTCTTAGAGAGTTTTGGTTTTCTCTCACAGCATCGTTCGGTGTATCTCCAATTTTCAGA
TGCATCCCTGAATAGTCAGGTTTTTTTACAACGTATAGATGGGCAACATTATCTTAATCAGGGCATGACAGCCGAGCTGA
TTTGTTTATCAACCAATGCTCATATTCCACTCAAAACATTTATTGGGGTGCAGGTTGCTGTTGATCAAGTAACAGATCGA
GGAAGTTTTTTCCGTACTACAGGTATTATTACTGGAGCATCACAAGGGCAAAGTGATGGTGCTTTAACTTTATATAAACT
TGCCATAAGTGATCCAACTTATCTCTGGCATAAACGCAGAAATAGCCGTGTGTTTATGAACAAGAGTGTGAAAGAAATTA
GTGAAATTCTGTTTCAAGAGTGGCAAGGAAAAAGCCCATTATTTGCTTCTAGTCTGACTCTAGATTTGAGCGGCTTGAAA
CAGACCTACGATGTCCGTCCTTTTGTTATGCAGCTCAATGAAAGCGACTATGACTTTTTAACTCGCTTATGGCGTAGTGA
AGGTATTAGCTGGCTCATTGATGAAGCAGAGCTTACCGTGGCGAGCAATATGGACAATATTCAGCCACAAAAATTACGTC
TTATTGATGATAATAACCAATATCAAGCTCTGACAAGAAGAGCGATTCGTTACCACCGTAGTAGTGCAACTGAACAATTT
GATAGCATGACCAGCCTAATGGCTGATCGCAGTTTACAGCCGACTTCTATATTTGTGCAGCGTTGGCAGCCAGACGTATT
ACAACAAACAGATGGGGCAGGTAGTGTCCAAAGTAAACATCAGCATAGTACTAACTACGACAATCAAAGCTTAAGCCTTG
AAGAAGCATGGCACTTTAGTCCGGCATGGATGCAGGATTTAAATGGCGAAGATGGGGCAACCTCAGCAAGTAATCAACAA
CTAGAAAAGTTTAACCAGAACCTAAGTGCTTATTATGATGCTCAGTCTAAGCAGTTTATTGCAAAAACCACAGTACGAGA
TACACAAGTTGGCTATTGGTTTGAACTTAATGAACACCCTGAAATTGATCAGCATGAAAGCACAGATAAAGAGTTTTTAA
TCATAGGTAAGAATTATTATAACCAAAATAATTTACCCAAAGATTTAAACCAGCAAATCCAAACTTTACTTCAACAAAGC
GATTGGCAAGCGAGTAATACCGACGAGCGTCAAGCGAATCAGTTAATTTTACAACGTCGTTATATTCCAACAACACCAGC
TTATAACCCACAGACTCATAGCCCAGTCGCTCATCCACAACGTGCGAAAGTGGTGGGGCCTGAAGGTGAAGAAATCTATG
TAGATGAATGGGGGCGTATTAAAGTCCGCTTCCTGTTTACGCGTAGTGATGACCATAGTCATGATGGGGGCGCAGGGACC
AATAATAATGACACCGATTCAGCTTGGATTGACGTTCTAACGCCGTGGGCTGGAGAAGGTTATGGTGCACGGTTTTTACC
GCGTATCGGTGAGATTGTTGTTATTAACTTTTTTAATGGCGATATAGACCGACCGTTTGTGATGGGTAGAGTGCATGAGG
CCCAGCGCCATCCAACCAAATTTGATAATAAAGGAAAGCTTCCTGATACTAAAAAGCTCAGTGGTATCCGTTCTAAAGAA
GTGTCAGGTAGCGGTTTTGGGCAACTCAGATTTGATGATACACCGGGACAAATTAGTACCCAACTTCAAAGCAGTCATGG
TGCAAGCCAGTTAAATCTGGGGAAACTCAGTCACCCTAAAGATAAAGCAGAAAGTGAAGACCGCGGAGAAGGTTTTGAAC
TGCGTACGGATCAATGGGGAGCATTACGCGCGGGGCAAGGTCTATTGGTGTCCACCCATAAACAGGATAATGCCAAAGGC
GAACACCTTGACGCCGAAGTTGCCAAAAAGCAACTTGAAGGTAGTCAAACCAACAGTAAAGCACTGAGTGACATTGCTAA
AAACCAGAAAACCGATGAAATAGAGTCGCTTGAACAGTTAAAAGATTTTGCCTCACAAATTCAGCAGCAAATCGCTAAAT
TTGAAAAAGCTTTACTCCTTTTAAGTTCACCGGATGGTATAGCTTTAAGTAGTTCAGAAGATATTCACATCTCGGCTGAT
GCACAAATTAATCAGATTGCTGGAGACAGCATTAATATAAGTACTCAAAAGAATGTGATTGCCCATGCCCAAAATAGGCT
CAGCCGCTTTGCAGCACAAAGTGGACTAAAAGCTGTTGCTGCACAAGGCAAGGTAGAAATACAAGCTCAGGCAGATGCAC
TGGATGTACTGAGTAAACTTGGAATTACTATTTCATCAACCGATGACAAGGTCATCATTAGTAGCCCGAAAGAAGTCAAA
ATCACGGGTGGCTCATCGCAAATTACTCTAAATGGTTCAGGAATCTTTCCGAAAACTGGTGGGAAATTCCAAGTCAATGC
TGGACAGCATTTGTTTATGGCAGGAGCAAGTGCAAACGCCAGTGCTCCTGAGTTGCCTAAAGCTAAACCTATGCAGGGAG
CATTGGAGCTACTTCGGAGTTATGGTGGAGATAACTTCTTCAAGCAAAATAGCTATAAAGTCATTGATTCATTAGGTAAG
CAAATTACGGGCAAACTCGATGGAAATGGTTTTGCACAAGTAACAGGTATTGCACCCGGACCAGCTAAAGTAGTTTTTGA
GAAGGATAATACAAGTGCATGGCTACAAAGCAGTGATTTTAAACGTAATTATACGTGGGCTGAACCAGTCAAAAGTGTTC
AAGGATTAATGAAAAATGCTCTAGAAGCAGTGGGGCAAAATACGATGTCTCAGTTGCAAAATAATCTTCTTTCAACGGAT
AAAAATAGTTTCAAAAACTTAGGTAAGAATACTTTAGCTAATTTAGCTGGCCAAACAGTAGGCCAGATTAAAAATCAGGT
AACTAATACTGCCCTTAATACTGTTTCAAAACAACTTAATCTCAATTTATCGGCCGATCAAATGAAACGTCTTGGCCAAA
TGGCGACAAATCCAAGTCAATCTCTTGAAATGCTTAAAGAGCAGGGCGATTTAGATACTTTTGTACGGTCAAAAAAGTAG