Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   ABC810_RS01045 Genome accession   NZ_CP155532
Coordinates   212329..213270 (+) Length   313 a.a.
NCBI ID   WP_000249573.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain SP264     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 207329..218270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABC810_RS01025 (ABC810_01025) - 207395..209212 (+) 1818 WP_001220865.1 acyltransferase family protein -
  ABC810_RS01030 (ABC810_01030) nagA 209365..210516 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  ABC810_RS01035 (ABC810_01035) - 210679..211737 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  ABC810_RS01040 (ABC810_01040) - 211888..212253 (+) 366 WP_000286415.1 DUF1033 family protein -
  ABC810_RS01045 (ABC810_01045) comGA/cglA/cilD 212329..213270 (+) 942 WP_000249573.1 competence type IV pilus ATPase ComGA Machinery gene
  ABC810_RS01050 (ABC810_01050) comGB/cglB 213218..214234 (+) 1017 WP_078134418.1 competence type IV pilus assembly protein ComGB Machinery gene
  ABC810_RS01055 (ABC810_01055) comGC/cglC 214236..214562 (+) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  ABC810_RS01060 (ABC810_01060) comGD/cglD 214555..214959 (+) 405 WP_000588030.1 competence type IV pilus minor pilin ComGD Machinery gene
  ABC810_RS01065 (ABC810_01065) comGE/cglE 214922..215224 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  ABC810_RS01070 (ABC810_01070) comGF/cglF 215187..215648 (+) 462 WP_000250539.1 competence type IV pilus minor pilin ComGF Machinery gene
  ABC810_RS01075 (ABC810_01075) comGG/cglG 215626..216072 (+) 447 WP_001811371.1 competence type IV pilus minor pilin ComGG Machinery gene
  ABC810_RS01080 (ABC810_01080) - 216175..216769 (+) 595 Protein_196 methyltransferase domain-containing protein -
  ABC810_RS01085 (ABC810_01085) comYH 216830..217783 (+) 954 WP_000345133.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35564.49 Da        Isoelectric Point: 6.0083

>NTDB_id=997078 ABC810_RS01045 WP_000249573.1 212329..213270(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain SP264]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=997078 ABC810_RS01045 WP_000249573.1 212329..213270(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain SP264]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment