Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   QUD94_RS08050 Genome accession   NZ_AP027136
Coordinates   1638979..1639254 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain JARB-HU0796     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1603286..1638955 1638979..1639254 flank 24


Gene organization within MGE regions


Location: 1603286..1639254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD94_RS07795 (JARBHU0796_15130) - 1603286..1604293 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  QUD94_RS07800 (JARBHU0796_15140) comGG 1604422..1604775 (-) 354 WP_010710571.1 competence type IV pilus minor pilin ComGG -
  QUD94_RS07805 (JARBHU0796_15150) comGF 1604775..1605209 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  QUD94_RS07810 (JARBHU0796_15160) - 1605199..1605600 (-) 402 WP_002397440.1 type II secretion system protein -
  QUD94_RS07815 (JARBHU0796_15170) - 1605721..1606206 (-) 486 WP_033918797.1 hypothetical protein -
  QUD94_RS07820 (JARBHU0796_15180) - 1606282..1606752 (-) 471 WP_033918796.1 GNAT family N-acetyltransferase -
  QUD94_RS07825 (JARBHU0796_15190) - 1606874..1608133 (-) 1260 WP_286301418.1 LysM peptidoglycan-binding domain-containing protein -
  QUD94_RS07830 (JARBHU0796_15200) - 1608134..1608529 (-) 396 WP_010784229.1 phage holin family protein -
  QUD94_RS07835 (JARBHU0796_15210) - 1608563..1610980 (-) 2418 WP_052141340.1 SGNH/GDSL hydrolase family protein -
  QUD94_RS07840 (JARBHU0796_15220) - 1610997..1613834 (-) 2838 WP_262140466.1 phage tail tip lysozyme -
  QUD94_RS07845 (JARBHU0796_15230) - 1613831..1614535 (-) 705 WP_010784234.1 hypothetical protein -
  QUD94_RS07850 (JARBHU0796_15240) - 1614538..1616835 (-) 2298 WP_033918684.1 phage tail tape measure protein -
  QUD94_RS07855 (JARBHU0796_15250) - 1617027..1617482 (-) 456 WP_002395308.1 hypothetical protein -
  QUD94_RS07860 (JARBHU0796_15260) - 1617482..1618129 (-) 648 WP_080296635.1 phage tail protein -
  QUD94_RS07865 (JARBHU0796_15270) - 1618126..1618488 (-) 363 WP_033918686.1 HK97-gp10 family putative phage morphogenesis protein -
  QUD94_RS07870 (JARBHU0796_15280) - 1618478..1618816 (-) 339 WP_033918687.1 hypothetical protein -
  QUD94_RS07875 (JARBHU0796_15290) - 1618806..1619132 (-) 327 WP_033918688.1 phage head closure protein -
  QUD94_RS07880 (JARBHU0796_15300) - 1619110..1619391 (-) 282 WP_002395304.1 phage gp6-like head-tail connector protein -
  QUD94_RS07885 (JARBHU0796_15310) - 1619394..1620755 (-) 1362 WP_016617775.1 phage major capsid protein -
  QUD94_RS07890 (JARBHU0796_15320) - 1620767..1621339 (-) 573 WP_002393031.1 HK97 family phage prohead protease -
  QUD94_RS07895 (JARBHU0796_15330) - 1621326..1622537 (-) 1212 WP_029677624.1 phage portal protein -
  QUD94_RS07900 (JARBHU0796_15340) - 1622556..1624283 (-) 1728 WP_286301447.1 terminase TerL endonuclease subunit -
  QUD94_RS07905 (JARBHU0796_15350) - 1624280..1624732 (-) 453 WP_002393034.1 P27 family phage terminase small subunit -
  QUD94_RS07910 - 1624846..1625211 (-) 366 WP_016617778.1 HNH endonuclease signature motif containing protein -
  QUD94_RS07915 (JARBHU0796_15370) - 1625211..1625591 (-) 381 WP_016617779.1 Rho termination factor N-terminal domain-containing protein -
  QUD94_RS07925 (JARBHU0796_15380) - 1626120..1626587 (-) 468 WP_002373959.1 ArpU family phage packaging/lysis transcriptional regulator -
  QUD94_RS07930 (JARBHU0796_15390) - 1626771..1626998 (-) 228 WP_016617780.1 hypothetical protein -
  QUD94_RS07935 (JARBHU0796_15410) - 1627193..1627387 (-) 195 Protein_1540 YopX family protein -
  QUD94_RS07940 (JARBHU0796_15420) - 1627561..1627785 (+) 225 WP_010817438.1 hypothetical protein -
  QUD94_RS07945 (JARBHU0796_15430) - 1627843..1628049 (-) 207 WP_016617783.1 hypothetical protein -
  QUD94_RS07950 (JARBHU0796_15440) - 1628065..1628670 (-) 606 WP_010817440.1 hypothetical protein -
  QUD94_RS07955 (JARBHU0796_15450) - 1628719..1629099 (+) 381 WP_286301458.1 hypothetical protein -
  QUD94_RS07960 (JARBHU0796_15460) - 1629100..1629570 (-) 471 WP_010817441.1 DUF1064 domain-containing protein -
  QUD94_RS07965 (JARBHU0796_15480) - 1629719..1629988 (-) 270 WP_010817442.1 hypothetical protein -
  QUD94_RS07970 (JARBHU0796_15490) - 1630127..1630966 (-) 840 WP_033918690.1 ATP-binding protein -
  QUD94_RS07975 (JARBHU0796_15500) - 1630978..1631757 (-) 780 WP_033918691.1 DnaD domain protein -
  QUD94_RS07980 (JARBHU0796_15510) - 1631772..1632587 (-) 816 WP_033918692.1 PD-(D/E)XK nuclease-like domain-containing protein -
  QUD94_RS07985 (JARBHU0796_15520) - 1632550..1633521 (-) 972 WP_033918693.1 RecT family recombinase -
  QUD94_RS07990 (JARBHU0796_15530) - 1633614..1633970 (-) 357 WP_033918694.1 hypothetical protein -
  QUD94_RS07995 (JARBHU0796_15540) - 1633973..1634197 (-) 225 WP_033918695.1 hypothetical protein -
  QUD94_RS08000 (JARBHU0796_15550) - 1634197..1634517 (-) 321 WP_033918696.1 hypothetical protein -
  QUD94_RS08005 (JARBHU0796_15560) - 1634585..1634773 (-) 189 WP_033918697.1 hypothetical protein -
  QUD94_RS08010 (JARBHU0796_15570) - 1634770..1634991 (-) 222 WP_229294412.1 aspartate decarboxylase -
  QUD94_RS08015 (JARBHU0796_15580) - 1635007..1635318 (-) 312 WP_016617789.1 hypothetical protein -
  QUD94_RS08020 (JARBHU0796_15590) - 1635330..1635521 (-) 192 WP_010712202.1 hypothetical protein -
  QUD94_RS08025 (JARBHU0796_15600) - 1635810..1636142 (+) 333 WP_016627611.1 helix-turn-helix transcriptional regulator -
  QUD94_RS08030 (JARBHU0796_15610) - 1636160..1636504 (+) 345 WP_033918698.1 ImmA/IrrE family metallo-endopeptidase -
  QUD94_RS08035 (JARBHU0796_15620) - 1636538..1637266 (+) 729 WP_033918699.1 potassium channel family protein -
  QUD94_RS08040 (JARBHU0796_15630) - 1637363..1638511 (+) 1149 WP_033918700.1 site-specific integrase -
  QUD94_RS08045 (JARBHU0796_15640) comGD 1638539..1638982 (-) 444 WP_129992406.1 competence type IV pilus minor pilin ComGD -
  QUD94_RS08050 (JARBHU0796_15650) comGC/cglC 1638979..1639254 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=99690 QUD94_RS08050 WP_002356991.1 1638979..1639254(-) (comGC/cglC) [Enterococcus faecalis strain JARB-HU0796]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=99690 QUD94_RS08050 WP_002356991.1 1638979..1639254(-) (comGC/cglC) [Enterococcus faecalis strain JARB-HU0796]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396

Multiple sequence alignment