Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AAG092_RS07480 Genome accession   NZ_CP154874
Coordinates   1541462..1542685 (+) Length   407 a.a.
NCBI ID   WP_110680822.1    Uniprot ID   A0A2V4LBW7
Organism   Pseudomonas alcaligenes strain Med1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1536269..1545789 1541462..1542685 within 0


Gene organization within MGE regions


Location: 1536269..1545789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG092_RS07450 (AAG092_07400) - 1536269..1537162 (-) 894 WP_373389177.1 ABC transporter ATP-binding protein -
  AAG092_RS07455 (AAG092_07405) - 1537159..1537995 (-) 837 WP_110680826.1 hypothetical protein -
  AAG092_RS07460 (AAG092_07410) - 1537992..1538789 (-) 798 WP_110680825.1 ABC transporter permease subunit -
  AAG092_RS07465 (AAG092_07415) - 1538861..1539301 (-) 441 WP_110680824.1 hypothetical protein -
  AAG092_RS07470 (AAG092_07420) pilA 1539321..1539503 (-) 183 WP_110680823.1 type IV pilin protein Machinery gene
  AAG092_RS07475 (AAG092_07425) pilB 1539749..1541455 (+) 1707 WP_373389568.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG092_RS07480 (AAG092_07430) pilC 1541462..1542685 (+) 1224 WP_110680822.1 type II secretion system F family protein Machinery gene
  AAG092_RS07485 (AAG092_07435) pilD 1542685..1543557 (+) 873 WP_373389178.1 A24 family peptidase Machinery gene
  AAG092_RS07490 (AAG092_07440) coaE 1543554..1544165 (+) 612 WP_373389179.1 dephospho-CoA kinase -
  AAG092_RS07495 (AAG092_07445) yacG 1544162..1544356 (+) 195 WP_110680819.1 DNA gyrase inhibitor YacG -
  AAG092_RS07500 (AAG092_07450) - 1544360..1544575 (-) 216 WP_373389180.1 hypothetical protein -
  AAG092_RS07505 (AAG092_07455) - 1544681..1545367 (-) 687 WP_373389181.1 energy-coupling factor ABC transporter permease -
  AAG092_RS07510 (AAG092_07460) - 1545364..1545789 (-) 426 WP_373389182.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44219.89 Da        Isoelectric Point: 9.5142

>NTDB_id=994320 AAG092_RS07480 WP_110680822.1 1541462..1542685(+) (pilC) [Pseudomonas alcaligenes strain Med1]
MADKALKTSTFQWEGKDKRGNKLKGETNSTSIALVRAQLRSQGIAAEKVKKKPKPLFGGAGKKIKPMDICLFTRQMATMM
GAGVPLLQSFDIIADGVENANMRTLIENIKQEVAAGNSLANSLRKFPLYFDDLYCNLVDSGEQSGSLEQLLDRIATYKEK
TEALKAKIKKAMTYPIAVIVVAVIVTAILLIKVVPTFQGVFAGFGAELPAFTMMVIHMSEFLQAWWFAFLIGIFAFGFGF
KKLHQSSEKFRDGMDRTLLKLPIFGDILYKSVVARFGRTLATTFSAGVPLVDALDSVSGATGNVVFRNAVNKIKQDVSTG
MQLNFSMRTTGVFPAMATQMTAIGEESGALDEMLDKVASYYEAEVDNAVDNLTTLLEPLIMAVLGVLVGGLIIAMYLPIF
SLGGVIG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=994320 AAG092_RS07480 WP_110680822.1 1541462..1542685(+) (pilC) [Pseudomonas alcaligenes strain Med1]
ATGGCGGATAAAGCGCTCAAGACCAGCACCTTCCAATGGGAGGGCAAGGACAAGAGAGGCAACAAGCTGAAGGGCGAGAC
CAACAGCACATCGATCGCGCTGGTGCGAGCGCAACTTCGCTCACAGGGCATCGCCGCCGAAAAGGTCAAGAAAAAACCCA
AACCACTCTTTGGCGGCGCCGGCAAGAAAATCAAACCTATGGACATTTGCCTGTTCACTAGGCAGATGGCAACCATGATG
GGAGCAGGCGTTCCCTTGCTCCAATCCTTCGACATCATCGCCGATGGAGTCGAAAACGCCAACATGCGCACCCTGATCGA
GAACATTAAACAGGAGGTAGCCGCTGGTAACAGCCTTGCCAATTCCCTGCGCAAGTTCCCGCTTTATTTCGACGATCTAT
ATTGCAACCTGGTTGACTCAGGCGAGCAGTCAGGCTCTCTGGAACAGTTGCTGGACCGTATCGCCACCTATAAAGAAAAG
ACCGAAGCACTCAAGGCAAAGATAAAAAAGGCAATGACCTACCCGATCGCCGTGATAGTGGTTGCGGTGATTGTTACCGC
AATTCTGCTGATCAAGGTTGTGCCGACATTCCAGGGTGTCTTTGCCGGCTTTGGTGCCGAGTTACCGGCCTTCACCATGA
TGGTCATTCATATGTCCGAATTCCTACAGGCATGGTGGTTTGCATTCCTGATCGGCATTTTCGCCTTCGGCTTCGGCTTT
AAGAAGCTTCACCAGAGCAGCGAAAAATTCCGCGACGGTATGGACAGGACACTTCTTAAACTTCCTATCTTCGGCGACAT
TCTCTACAAGTCTGTAGTGGCGCGCTTCGGTCGAACACTCGCAACCACCTTCTCTGCAGGCGTGCCGCTAGTCGACGCAC
TGGACTCTGTATCAGGCGCCACCGGCAACGTGGTATTCCGAAATGCGGTGAACAAGATCAAGCAGGATGTTTCCACAGGC
ATGCAGCTGAACTTTTCTATGCGCACTACAGGTGTATTCCCGGCAATGGCAACCCAGATGACTGCCATCGGCGAAGAGTC
TGGTGCGCTCGATGAAATGCTAGATAAAGTCGCTTCTTACTATGAAGCAGAAGTCGACAATGCCGTGGACAACCTGACAA
CCCTACTAGAGCCCCTGATTATGGCCGTGCTGGGCGTGCTGGTCGGCGGCCTGATCATCGCCATGTACCTGCCGATCTTC
AGCCTGGGCGGTGTAATCGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(278-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2V4LBW7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.877

99.754

0.747
  pilC Acinetobacter baumannii D1279779

58.663

99.263

0.582
  pilC Acinetobacter baylyi ADP1

58.374

99.754

0.582
  pilC Legionella pneumophila strain ERS1305867

55.138

98.034

0.541
  pilG Neisseria gonorrhoeae MS11

44.307

99.263

0.44
  pilG Neisseria meningitidis 44/76-A

44.307

99.263

0.44
  pilC Vibrio cholerae strain A1552

43.35

99.754

0.432
  pilC Vibrio campbellii strain DS40M4

41.278

100

0.413
  pilC Thermus thermophilus HB27

37.931

99.754

0.378

Multiple sequence alignment