Detailed information NTG0994319

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: AAG092_RS07475
• Genome accession: NZ_CP154874
• Coordinates: 1539749..1541455 (+)
• Length: 568 a.a.
• NCBI ID: WP_373389568.1
• Uniprot ID: -
• Organism: Pseudomonas alcaligenes strain Med1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	1536269..1545789	1539749..1541455	within	0

Gene organization within MGE regions

Location: 1536269..1545789

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AAG092_RS07450 (AAG092_07400)	-	1536269..1537162 (-)	894	WP_373389177.1	ABC transporter ATP-binding protein	-
AAG092_RS07455 (AAG092_07405)	-	1537159..1537995 (-)	837	WP_110680826.1	hypothetical protein	-
AAG092_RS07460 (AAG092_07410)	-	1537992..1538789 (-)	798	WP_110680825.1	ABC transporter permease subunit	-
AAG092_RS07465 (AAG092_07415)	-	1538861..1539301 (-)	441	WP_110680824.1	hypothetical protein	-
AAG092_RS07470 (AAG092_07420)	pilA	1539321..1539503 (-)	183	WP_110680823.1	type IV pilin protein	Machinery gene
AAG092_RS07475 (AAG092_07425)	pilB	1539749..1541455 (+)	1707	WP_373389568.1	type IV-A pilus assembly ATPase PilB	Machinery gene
AAG092_RS07480 (AAG092_07430)	pilC	1541462..1542685 (+)	1224	WP_110680822.1	type II secretion system F family protein	Machinery gene
AAG092_RS07485 (AAG092_07435)	pilD	1542685..1543557 (+)	873	WP_373389178.1	A24 family peptidase	Machinery gene
AAG092_RS07490 (AAG092_07440)	coaE	1543554..1544165 (+)	612	WP_373389179.1	dephospho-CoA kinase	-
AAG092_RS07495 (AAG092_07445)	yacG	1544162..1544356 (+)	195	WP_110680819.1	DNA gyrase inhibitor YacG	-
AAG092_RS07500 (AAG092_07450)	-	1544360..1544575 (-)	216	WP_373389180.1	hypothetical protein	-
AAG092_RS07505 (AAG092_07455)	-	1544681..1545367 (-)	687	WP_373389181.1	energy-coupling factor ABC transporter permease	-
AAG092_RS07510 (AAG092_07460)	-	1545364..1545789 (-)	426	WP_373389182.1	GNAT family N-acetyltransferase	-

Sequence

Protein

• Length: 568 a.a.
• Molecular weight: 62535.43 Da
• Isoelectric Point: 5.2231

>NTDB_id=994319 AAG092_RS07475 WP_373389568.1 1539749..1541455(+) (pilB) [Pseudomonas alcaligenes strain Med1]
MSNSPVLTGLARQLVLAELIDETTAGRAVQQAQRNQIPLVTYLVQNKLVNSTALASQAAEQFGVPYFDLSALDKDQQPKD
LISEKLARQHRALPLWKRGNKLYIGMSDPTLQQAVTDIQFSTGLNTELVLVQDDRLDAAIDRYFESDNKTMEGLGDIDLE
DGLEIESGDQEREDNSTGGDADDAPVVKFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGVLHEVAKPPIQLASRISA
RLKVMASMDISERRKPQDGRIKMKISKTKAIDFRVNTCPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKQLYMDALAKP
QGMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIR
DLETASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCSNCKKEVSIPPEVQVAEGF
PAERVGTFKLYGPVGCDHCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIADQMRKDGFNDLRTSGLLKAIQGLTSLE
EINRVTKD

Nucleotide

• Length: 1707 bp

>NTDB_id=994319 AAG092_RS07475 WP_373389568.1 1539749..1541455(+) (pilB) [Pseudomonas alcaligenes strain Med1]
ATGAGCAACTCTCCCGTCCTGACCGGCCTTGCCAGGCAACTGGTGCTGGCCGAACTGATCGATGAAACGACCGCCGGCCG
GGCGGTTCAACAAGCCCAGCGCAACCAGATCCCACTGGTCACCTATTTGGTACAAAACAAACTGGTCAACAGCACCGCAT
TGGCCAGCCAGGCTGCCGAGCAGTTCGGCGTCCCCTACTTTGACCTGAGCGCACTGGATAAAGATCAACAACCAAAAGAC
CTGATCAGCGAGAAGCTGGCGCGCCAGCATCGCGCCTTGCCACTGTGGAAGCGTGGCAACAAGCTGTATATAGGCATGTC
AGACCCGACCCTGCAGCAGGCGGTAACAGACATCCAGTTCAGCACCGGGCTCAACACCGAATTGGTACTGGTGCAGGATG
ACCGCCTGGATGCCGCCATTGATCGCTACTTCGAAAGCGACAACAAGACCATGGAGGGTCTGGGCGACATCGATCTGGAA
GACGGCCTGGAAATCGAGAGCGGGGATCAAGAACGGGAGGACAACTCTACCGGCGGCGATGCCGACGATGCCCCAGTGGT
CAAATTCGTCAACAAGATGCTGCTGGATGCCATTAAAGGCGGGTCCTCGGATCTGCACTTCGAACCCTATGAAAAAGTGT
ATCGCGTACGTTTCCGTACCGACGGCGTCCTGCACGAGGTGGCCAAGCCCCCCATTCAACTGGCCTCCCGCATTTCGGCG
CGCCTGAAGGTCATGGCCAGCATGGACATATCCGAACGGCGCAAGCCGCAAGACGGCCGAATCAAGATGAAAATCTCGAA
GACCAAGGCCATCGACTTCCGCGTCAACACCTGCCCGACCCTCTGGGGTGAGAAAATCGTCATGCGTATTCTCGACCCCT
CCAGCGCGCAGATGGGGATTGACGCACTGGGCTATGAGGAAGACCAGAAGCAACTCTACATGGATGCCCTGGCCAAACCC
CAGGGGATGATTCTGGTCACCGGCCCCACCGGTTCGGGGAAGACGGTATCGCTCTATACCGGCCTGAACATTCTCAACAC
GGTGGACGTGAACATCTCCACCGCCGAAGACCCAGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCA
AGCAGGGTATGGACTTCGCCCAGGCGCTACGAGCTTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGCGAGATCCGC
GACCTGGAAACCGCATCAATCGCCATCAAGGCAGCCCAGACTGGTCACATGGTGATGTCTACCCTTCACACCAACAGCGC
AGCAGAAACCCTCACCCGCCTGCGCAATATGGGCGTCCCCTCCTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCC
AACGACTGGCCCGCAAGCTATGTAGCAACTGCAAGAAAGAAGTGAGCATTCCACCCGAGGTACAGGTCGCAGAGGGATTT
CCTGCTGAACGGGTTGGCACATTCAAGCTCTATGGCCCAGTTGGCTGCGATCACTGCAAAGGCGGCTACAAAGGGCGCGT
AGGGATTTATGAAGTGGTTAAAAACACGCCAGCGCTTCAGCGGATTATCATGGAAGAAGGCAACTCCATAGATATCGCCG
ACCAGATGCGCAAAGACGGCTTCAACGACCTGCGCACCTCGGGCCTACTCAAGGCCATACAAGGCCTGACCAGCCTCGAG
GAAATCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-147)

• Type II/IV secretion system protein (T2SSE) (192-459)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.947	100	0.592
pilB	Acinetobacter baylyi ADP1	56.316	100	0.565
pilB	Legionella pneumophila strain ERS1305867	54.93	100	0.549
pilB	Vibrio cholerae strain A1552	49.476	100	0.498
pilB	Vibrio campbellii strain DS40M4	48.845	99.12	0.484
pilF	Neisseria gonorrhoeae MS11	48.142	99.472	0.479
pilB	Vibrio parahaemolyticus RIMD 2210633	50.464	94.894	0.479
pilF	Thermus thermophilus HB27	36.907	100	0.37
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.13	93.134	0.364