Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QMG91_RS16835 Genome accession   NZ_AP027049
Coordinates   3672128..3673876 (-) Length   582 a.a.
NCBI ID   WP_281827768.1    Uniprot ID   -
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3667128..3678876
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG91_RS16815 (TUMSATVNIG2_32650) - 3667852..3668685 (-) 834 WP_004405319.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  QMG91_RS16820 (TUMSATVNIG2_32660) - 3668755..3670278 (-) 1524 WP_281827763.1 AAA family ATPase -
  QMG91_RS16825 (TUMSATVNIG2_32670) aroB 3670309..3671397 (-) 1089 WP_281827765.1 3-dehydroquinate synthase -
  QMG91_RS16830 (TUMSATVNIG2_32680) aroK 3671420..3671938 (-) 519 WP_004405322.1 shikimate kinase AroK -
  QMG91_RS16835 (TUMSATVNIG2_32690) pilQ 3672128..3673876 (-) 1749 WP_281827768.1 type IV pilus secretin PilQ Machinery gene
  QMG91_RS16840 (TUMSATVNIG2_32700) pilP 3673913..3674425 (-) 513 WP_022603536.1 pilus assembly protein PilP Machinery gene
  QMG91_RS16845 (TUMSATVNIG2_32710) pilO 3674418..3675005 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  QMG91_RS16850 (TUMSATVNIG2_32720) pilN 3675002..3675562 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  QMG91_RS16855 (TUMSATVNIG2_32730) pilM 3675555..3676571 (-) 1017 WP_004405331.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 62840.07 Da        Isoelectric Point: 4.2852

>NTDB_id=99344 QMG91_RS16835 WP_281827768.1 3672128..3673876(-) (pilQ) [Vibrio nigripulchritudo strain TUMSAT-V.nig2]
MAEFCRAHIYRCALLIFIGLFSTTGVHAQSNEFVGIDFRLSKEKHALLVVELASNAAAVDIQPMPDGLNISLHETSVVDE
KLFVVDVSDFATSVENVEVFREGSNTRLFASTNAAYNYDYRLRDRFLEITVSAKPKDEPEQASILEKEGKLISINFQDIP
VRSVLQLIADYNGFNLVVSDSVTGNLTLKVDDVPWQQVLDIILKVKGLDKRVEGNVILVAPKEEFDQREQQDLEQERLAQ
ELGTLESKIVEVKFAKASDIAEMIGGDGEISMLSERGSISIDERTNSLLVRDLPENISVIEDIVESLDIPVKQVQIEARI
VTVTEGELEELGVRWGFSSTSGPNSVGATIENNLATVGLYQAGGSGGDSGGEDGGEGGGGGSTSIDDFLAVNLGATNANA
ASIAFQVAKLGSDTLLDLELSALQTENKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATTVAFRKAVLSLKVTPQ
ITPDNKLVLDLSVTQDRPGKVVKTGNGEALAIETQRIGTQVLVDNGETVVLGGIYQQSVTNTVDKVPLLGDLPLLGALFR
RSYENLGKSELLIFITPKVVLQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=99344 QMG91_RS16835 WP_281827768.1 3672128..3673876(-) (pilQ) [Vibrio nigripulchritudo strain TUMSAT-V.nig2]
ATGGCGGAGTTTTGTCGGGCGCATATATATCGTTGTGCTCTGCTTATCTTTATCGGGTTGTTTTCGACAACCGGGGTTCA
TGCCCAGTCCAACGAATTTGTTGGTATCGATTTTAGGCTCAGTAAAGAAAAACATGCTCTGCTGGTCGTGGAGTTAGCAT
CGAATGCGGCAGCGGTAGACATTCAGCCGATGCCCGATGGCTTAAACATCTCCCTGCATGAAACCAGCGTGGTGGACGAA
AAGCTTTTCGTGGTCGACGTATCCGATTTTGCAACCAGCGTAGAAAATGTGGAAGTGTTCCGTGAGGGCTCTAACACACG
TTTATTTGCCAGCACAAACGCGGCTTATAACTACGACTACCGCCTACGGGATCGTTTTCTGGAAATCACCGTCTCTGCGA
AACCCAAAGACGAACCAGAGCAGGCGAGCATTCTAGAAAAAGAAGGCAAGCTTATTTCTATTAACTTCCAGGACATCCCT
GTTCGAAGCGTATTACAGCTTATCGCTGACTATAACGGGTTCAATCTGGTAGTTTCTGACTCAGTCACAGGCAACTTAAC
GCTGAAGGTAGATGATGTACCGTGGCAGCAAGTGCTAGACATCATCCTGAAAGTAAAAGGGTTGGATAAGCGTGTTGAAG
GTAACGTAATTCTGGTTGCCCCAAAAGAAGAGTTTGATCAAAGAGAGCAGCAAGATCTTGAACAAGAACGCTTGGCGCAA
GAGCTCGGTACGCTGGAATCCAAAATCGTTGAAGTAAAGTTTGCCAAAGCGTCTGACATTGCGGAAATGATCGGCGGCGA
CGGTGAAATTTCCATGCTTTCTGAGCGCGGAAGTATTTCCATTGATGAACGTACCAATTCCCTTCTGGTACGCGACTTGC
CGGAAAACATCAGCGTTATTGAAGATATTGTGGAATCTCTCGATATTCCTGTTAAACAGGTTCAGATAGAAGCTCGTATT
GTGACTGTCACGGAAGGTGAACTTGAAGAGTTAGGTGTCCGCTGGGGCTTCAGTTCAACAAGTGGACCAAACTCTGTGGG
CGCGACCATTGAAAATAACCTCGCGACGGTCGGTCTTTATCAGGCTGGTGGCTCAGGTGGTGATTCTGGAGGCGAAGACG
GCGGTGAAGGTGGCGGCGGAGGCAGCACCAGTATTGATGACTTCCTTGCCGTCAATCTGGGAGCGACAAATGCCAATGCA
GCCAGTATCGCGTTCCAAGTGGCAAAACTTGGCTCTGACACCCTGTTGGATTTGGAACTGTCTGCTCTGCAAACGGAAAA
CAAAGCGGAAATCATCTCCAGCCCGAGACTGATTACCACCAACAAGAAGCCAGCCTACATCGAGCAAGGTACTGAAATTC
CTTACCTTGAAGCTTCATCCAGTGGTGCAACTACGGTCGCATTCCGTAAGGCTGTACTGAGCTTAAAGGTGACGCCACAG
ATTACCCCAGACAACAAACTGGTGCTGGATTTGAGTGTTACTCAGGACAGACCGGGCAAAGTCGTAAAAACAGGCAATGG
TGAAGCCCTGGCCATTGAAACTCAAAGAATTGGTACTCAGGTGTTGGTAGACAATGGAGAAACGGTGGTTCTTGGGGGTA
TTTATCAACAAAGTGTGACAAATACGGTCGATAAAGTGCCACTTTTGGGTGATTTACCTCTGTTAGGTGCACTTTTCCGT
CGAAGCTACGAAAATTTAGGAAAAAGCGAACTCCTTATTTTCATTACGCCAAAGGTCGTTCTACAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(174-222)

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(248-313)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

72.172

95.704

0.691
  pilQ Vibrio cholerae strain A1552

66.214

100

0.67
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

66.214

100

0.67
  pilQ Legionella pneumophila strain Lp02

40.141

97.595

0.392
  pilQ Legionella pneumophila strain ERS1305867

40.141

97.595

0.392
  pilQ Pseudomonas aeruginosa PAK

39.894

96.907

0.387
  comQ Acinetobacter baylyi ADP1

38.004

98.11

0.373
  pilQ Acinetobacter baumannii D1279779

36.486

100

0.371
  pilQ Acinetobacter baumannii strain A118

36.486

100

0.371

Multiple sequence alignment