Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   QMG91_RS16850 Genome accession   NZ_AP027049
Coordinates   3675002..3675562 (-) Length   186 a.a.
NCBI ID   WP_004405329.1    Uniprot ID   U4KA40
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3670002..3680562
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG91_RS16825 (TUMSATVNIG2_32670) aroB 3670309..3671397 (-) 1089 WP_281827765.1 3-dehydroquinate synthase -
  QMG91_RS16830 (TUMSATVNIG2_32680) aroK 3671420..3671938 (-) 519 WP_004405322.1 shikimate kinase AroK -
  QMG91_RS16835 (TUMSATVNIG2_32690) pilQ 3672128..3673876 (-) 1749 WP_281827768.1 type IV pilus secretin PilQ Machinery gene
  QMG91_RS16840 (TUMSATVNIG2_32700) pilP 3673913..3674425 (-) 513 WP_022603536.1 pilus assembly protein PilP Machinery gene
  QMG91_RS16845 (TUMSATVNIG2_32710) pilO 3674418..3675005 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  QMG91_RS16850 (TUMSATVNIG2_32720) pilN 3675002..3675562 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  QMG91_RS16855 (TUMSATVNIG2_32730) pilM 3675555..3676571 (-) 1017 WP_004405331.1 type IV pilus assembly protein PilM Machinery gene
  QMG91_RS16860 (TUMSATVNIG2_32740) - 3676717..3679215 (+) 2499 WP_281827772.1 penicillin-binding protein 1A -
  QMG91_RS16865 (TUMSATVNIG2_32750) oxyR 3679270..3680151 (-) 882 WP_004405335.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21363.47 Da        Isoelectric Point: 7.4657

>NTDB_id=99347 QMG91_RS16850 WP_004405329.1 3675002..3675562(-) (pilN) [Vibrio nigripulchritudo strain TUMSAT-V.nig2]
MHSVNLLPWRDEMRARYRRQFFSYLAAAILAAVGVQWGVGVYFSQQMSVQEERNAELTRHISYLDQQLRGLSEVRDQHES
ILTRLRSVEALQIKRNKTTDFMSHLPDTIPPGVYVDKIEMNDFEVEVNGISDSTANITTMLDSMERSAKLADVEMHSIVS
GKKLFGKVFKTFKVSFIFAPIKEEKS

Nucleotide


Download         Length: 561 bp        

>NTDB_id=99347 QMG91_RS16850 WP_004405329.1 3675002..3675562(-) (pilN) [Vibrio nigripulchritudo strain TUMSAT-V.nig2]
ATGCATAGCGTTAACTTACTGCCCTGGCGTGATGAAATGCGCGCTAGATACCGCCGCCAGTTTTTTTCTTATCTGGCTGC
CGCGATACTCGCCGCAGTCGGAGTGCAGTGGGGTGTTGGTGTTTATTTCTCACAGCAAATGAGTGTTCAGGAAGAACGCA
ATGCTGAGCTTACTCGTCACATCTCCTACTTAGACCAGCAACTACGCGGTTTGAGTGAAGTTCGCGATCAGCACGAGTCT
ATTCTTACCCGACTTCGCTCGGTCGAGGCGCTTCAAATCAAGCGCAACAAAACCACGGATTTTATGTCCCACCTGCCAGA
TACCATTCCACCAGGCGTTTACGTCGACAAAATTGAAATGAACGATTTTGAAGTTGAAGTGAATGGCATCAGTGACAGTA
CTGCCAATATCACCACAATGCTCGACAGTATGGAGCGATCCGCCAAGCTGGCAGATGTGGAAATGCACTCCATTGTTTCG
GGTAAGAAGCTGTTTGGCAAAGTATTCAAAACCTTCAAAGTATCCTTCATTTTTGCGCCTATCAAGGAAGAAAAATCATG
A

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U4KA40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

52.406

100

0.527
  pilN Vibrio cholerae strain A1552

53.591

97.312

0.522

Multiple sequence alignment