Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   QMG95_RS17265 Genome accession   NZ_AP027045
Coordinates   3734497..3736242 (-) Length   581 a.a.
NCBI ID   WP_200766247.1    Uniprot ID   -
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3729497..3741242
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG95_RS17245 (TUMSATVNIG1_33620) - 3730221..3731054 (-) 834 WP_004405319.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  QMG95_RS17250 (TUMSATVNIG1_33630) - 3731124..3732647 (-) 1524 WP_200766246.1 AAA family ATPase -
  QMG95_RS17255 (TUMSATVNIG1_33640) aroB 3732678..3733766 (-) 1089 WP_004405321.1 3-dehydroquinate synthase -
  QMG95_RS17260 (TUMSATVNIG1_33650) aroK 3733789..3734307 (-) 519 WP_004405322.1 shikimate kinase AroK -
  QMG95_RS17265 (TUMSATVNIG1_33660) pilQ 3734497..3736242 (-) 1746 WP_200766247.1 type IV pilus secretin PilQ family protein Machinery gene
  QMG95_RS17270 (TUMSATVNIG1_33670) pilP 3736279..3736791 (-) 513 WP_022593066.1 pilus assembly protein PilP Machinery gene
  QMG95_RS17275 (TUMSATVNIG1_33680) pilO 3736784..3737371 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  QMG95_RS17280 (TUMSATVNIG1_33690) pilN 3737368..3737928 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  QMG95_RS17285 (TUMSATVNIG1_33700) pilM 3737921..3738937 (-) 1017 WP_200766248.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 62783.02 Da        Isoelectric Point: 4.2852

>NTDB_id=99299 QMG95_RS17265 WP_200766247.1 3734497..3736242(-) (pilQ) [Vibrio nigripulchritudo strain TUMSAT-V.nig1]
MAEFCRAHIYRCALLIFIGLFSTTGVHAQSNEFVGIDFRLSKEKHALLVVELASNAAAVDIQPMPDGLNISLHETSVVDE
KLFVVDVSDFATSVENVEVFREGSNTRLFASTNAAYNYDYRLRDRFLEITVSAKPKDEPEQASILEKEGKLISINFQDIP
VRSVLQLIADYNGFNLVVSDSVTGNLTLKVDDVPWQQVLDIILKVKGLDKRVEGNVILVAPKEEFDQREQQDLEQERLAQ
ELGTLESKIVEVKFAKASDIAEMIGGDGEISMLSERGSISIDERTNSLLVRDLPENISVIEDIVESLDIPVKQVQIEARI
VTVTEGELEELGVRWGFSSTSGPNSVGATIENNLATVGLYQAGGSGGDSGGEGGDEGGGGSTSIDDFLAVNLGATNANAA
SIAFQVAKLGSDTLLDLELSALQTENKAEIISSPRLITTNKKPAYIEQGTEIPYLEASSSGATTVAFRKAVLSLKVTPQI
TPDNKLVLDLSVTQDRPGKVVKTGNGEALAIETQRIGTQVLVDNGETVVLGGIYQQSVTNTVDKVPLLGDLPLLGALFRR
SYENLGKSELLIFITPKVVLQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=99299 QMG95_RS17265 WP_200766247.1 3734497..3736242(-) (pilQ) [Vibrio nigripulchritudo strain TUMSAT-V.nig1]
ATGGCGGAGTTTTGTCGGGCGCATATATATCGTTGTGCTCTGCTTATCTTTATCGGGTTGTTTTCGACAACCGGGGTTCA
TGCCCAGTCCAACGAATTTGTTGGTATCGATTTTAGGCTCAGTAAAGAAAAACATGCTCTGCTGGTCGTGGAGTTAGCAT
CGAATGCGGCAGCGGTAGACATTCAGCCGATGCCCGATGGCTTAAACATCTCCCTGCATGAAACCAGCGTGGTGGACGAA
AAGCTTTTCGTGGTCGACGTATCCGATTTTGCTACCAGCGTAGAAAATGTGGAAGTGTTCCGTGAGGGCTCTAACACACG
TTTATTTGCCAGCACAAACGCGGCTTATAACTACGACTACCGCCTAAGGGATCGTTTTCTGGAAATCACCGTCTCTGCGA
AACCCAAAGACGAACCAGAGCAGGCGAGCATTCTAGAAAAAGAAGGCAAGCTTATTTCTATTAACTTCCAGGACATCCCT
GTTCGAAGCGTATTACAGCTTATCGCTGACTATAACGGGTTCAACCTGGTGGTATCTGACTCAGTCACAGGCAACTTAAC
GCTTAAGGTGGATGATGTGCCGTGGCAGCAAGTGCTAGACATCATCCTGAAAGTAAAAGGGTTGGATAAGCGTGTTGAAG
GCAACGTAATTCTGGTTGCCCCAAAAGAAGAGTTTGACCAAAGAGAGCAGCAAGATCTTGAACAAGAACGCTTGGCGCAA
GAGCTCGGTACGCTGGAATCCAAAATCGTTGAAGTAAAGTTTGCCAAAGCGTCTGACATTGCGGAAATGATCGGCGGCGA
CGGTGAAATTTCCATGCTTTCTGAGCGCGGAAGTATTTCCATTGATGAACGTACCAATTCCCTTCTGGTACGCGACTTAC
CGGAAAACATCAGCGTTATTGAAGATATTGTGGAATCTCTCGATATCCCTGTTAAACAGGTTCAGATAGAAGCTCGTATT
GTGACTGTCACAGAAGGTGAACTTGAAGAGTTGGGTGTCCGCTGGGGCTTCAGTTCAACAAGTGGACCAAACTCCGTGGG
CGCGACCATTGAAAATAACCTCGCGACGGTCGGTCTTTATCAGGCTGGTGGCTCAGGTGGTGATTCTGGAGGCGAAGGCG
GCGATGAAGGTGGCGGAGGCAGCACCAGTATTGATGACTTCCTTGCCGTCAATCTGGGAGCGACAAATGCCAATGCAGCC
AGCATCGCATTCCAAGTGGCAAAACTTGGCTCTGACACCCTGTTGGATTTGGAGCTGTCTGCCTTGCAAACGGAAAACAA
AGCGGAAATCATTTCCAGCCCGAGACTGATTACCACCAACAAGAAGCCAGCCTACATCGAGCAAGGTACTGAAATTCCTT
ACCTTGAAGCTTCATCCAGTGGTGCAACTACGGTCGCATTCCGTAAGGCTGTGCTGAGCTTAAAGGTGACGCCACAGATT
ACCCCAGACAACAAACTGGTGCTGGATTTGAGTGTTACTCAGGACAGACCCGGCAAAGTCGTAAAAACAGGCAATGGTGA
AGCCCTGGCCATTGAAACTCAAAGAATCGGTACTCAGGTGTTGGTAGACAATGGAGAAACGGTGGTTCTTGGGGGTATTT
ATCAACAAAGTGTGACAAATACGGTCGATAAAGTGCCACTTTTGGGTGATTTACCTCTGTTAGGTGCACTTTTCCGCCGA
AGCTACGAAAATTTAGGAAAAAGCGAACTCCTTATTTTCATTACGCCAAAGGTCGTTCTACAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(248-313)

Secretin and TonB N terminus short domain (STN)

(174-222)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

72.302

95.697

0.692
  pilQ Vibrio cholerae strain A1552

66.497

100

0.673
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

66.497

100

0.673
  pilQ Legionella pneumophila strain ERS1305867

40.283

97.418

0.392
  pilQ Legionella pneumophila strain Lp02

40.283

97.418

0.392
  pilQ Pseudomonas aeruginosa PAK

40

95.525

0.382
  comQ Acinetobacter baylyi ADP1

38.179

98.279

0.375
  pilQ Acinetobacter baumannii D1279779

36.348

100

0.373
  pilQ Acinetobacter baumannii strain A118

36.348

100

0.373

Multiple sequence alignment