Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   QMG95_RS17280 Genome accession   NZ_AP027045
Coordinates   3737368..3737928 (-) Length   186 a.a.
NCBI ID   WP_004405329.1    Uniprot ID   U4KA40
Organism   Vibrio nigripulchritudo strain TUMSAT-V.nig1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3732368..3742928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMG95_RS17255 (TUMSATVNIG1_33640) aroB 3732678..3733766 (-) 1089 WP_004405321.1 3-dehydroquinate synthase -
  QMG95_RS17260 (TUMSATVNIG1_33650) aroK 3733789..3734307 (-) 519 WP_004405322.1 shikimate kinase AroK -
  QMG95_RS17265 (TUMSATVNIG1_33660) pilQ 3734497..3736242 (-) 1746 WP_200766247.1 type IV pilus secretin PilQ family protein Machinery gene
  QMG95_RS17270 (TUMSATVNIG1_33670) pilP 3736279..3736791 (-) 513 WP_022593066.1 pilus assembly protein PilP Machinery gene
  QMG95_RS17275 (TUMSATVNIG1_33680) pilO 3736784..3737371 (-) 588 WP_004405328.1 type 4a pilus biogenesis protein PilO Machinery gene
  QMG95_RS17280 (TUMSATVNIG1_33690) pilN 3737368..3737928 (-) 561 WP_004405329.1 PilN domain-containing protein Machinery gene
  QMG95_RS17285 (TUMSATVNIG1_33700) pilM 3737921..3738937 (-) 1017 WP_200766248.1 type IV pilus assembly protein PilM Machinery gene
  QMG95_RS17290 (TUMSATVNIG1_33710) - 3739083..3741581 (+) 2499 WP_200766249.1 PBP1A family penicillin-binding protein -
  QMG95_RS17295 (TUMSATVNIG1_33720) oxyR 3741637..3742518 (-) 882 WP_004405335.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 186 a.a.        Molecular weight: 21363.47 Da        Isoelectric Point: 7.4657

>NTDB_id=99302 QMG95_RS17280 WP_004405329.1 3737368..3737928(-) (pilN) [Vibrio nigripulchritudo strain TUMSAT-V.nig1]
MHSVNLLPWRDEMRARYRRQFFSYLAAAILAAVGVQWGVGVYFSQQMSVQEERNAELTRHISYLDQQLRGLSEVRDQHES
ILTRLRSVEALQIKRNKTTDFMSHLPDTIPPGVYVDKIEMNDFEVEVNGISDSTANITTMLDSMERSAKLADVEMHSIVS
GKKLFGKVFKTFKVSFIFAPIKEEKS

Nucleotide


Download         Length: 561 bp        

>NTDB_id=99302 QMG95_RS17280 WP_004405329.1 3737368..3737928(-) (pilN) [Vibrio nigripulchritudo strain TUMSAT-V.nig1]
ATGCATAGCGTTAACTTACTGCCCTGGCGTGATGAAATGCGCGCCAGATACCGCCGCCAATTTTTTTCTTATCTGGCTGC
CGCGATACTTGCCGCAGTCGGAGTTCAGTGGGGTGTTGGTGTTTATTTCTCACAACAAATGAGTGTTCAGGAAGAACGCA
ATGCTGAGCTGACTCGCCACATCTCCTACTTAGACCAGCAACTACGCGGTTTGAGTGAGGTTCGCGATCAGCACGAGTCT
ATTCTTACCCGACTGCGCTCGGTCGAGGCGCTTCAAATCAAGCGCAACAAAACCACAGATTTTATGTCCCACCTGCCAGA
TACCATTCCACCGGGTGTTTACGTCGACAAAATTGAAATGAACGATTTTGAAGTTGAAGTGAATGGCATCAGTGACAGCA
CAGCCAATATCACCACAATGCTCGACAGTATGGAGCGCTCCGCCAAGCTGGCAGATGTGGAAATGCACTCCATTGTTTCG
GGTAAGAAGTTGTTTGGCAAAGTATTCAAAACCTTCAAAGTATCCTTCATTTTTGCGCCTATCAAGGAAGAAAAGTCATG
A

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(102-162)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U4KA40

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

52.406

100

0.527
  pilN Vibrio cholerae strain A1552

53.591

97.312

0.522

Multiple sequence alignment