Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AAD080_RS02100 Genome accession   NZ_CP152307
Coordinates   451733..452842 (+) Length   369 a.a.
NCBI ID   WP_342608030.1    Uniprot ID   -
Organism   Vibrio tritonius strain JCM 16457 isolate MA35     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 446733..457842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAD080_RS02070 - 447404..448003 (-) 600 WP_342608027.1 XTP/dITP diphosphatase -
  AAD080_RS02075 yggU 448162..448452 (-) 291 WP_068711922.1 DUF167 family protein YggU -
  AAD080_RS02080 - 448452..449009 (-) 558 WP_342608028.1 YggT family protein -
  AAD080_RS02085 proC 449080..449898 (-) 819 WP_342608029.1 pyrroline-5-carboxylate reductase -
  AAD080_RS02090 - 449952..450659 (-) 708 WP_068711928.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAD080_RS02095 pilT 450685..451722 (+) 1038 WP_068711930.1 type IV pilus twitching motility protein PilT Machinery gene
  AAD080_RS02100 pilU 451733..452842 (+) 1110 WP_342608030.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AAD080_RS02105 - 452866..453096 (+) 231 WP_342608031.1 hypothetical protein -
  AAD080_RS02110 ruvX 453172..453594 (-) 423 WP_342608032.1 Holliday junction resolvase RuvX -
  AAD080_RS02115 - 453675..454238 (-) 564 WP_342608705.1 YqgE/AlgH family protein -
  AAD080_RS02120 gshB 454279..455235 (-) 957 WP_068711936.1 glutathione synthase -
  AAD080_RS02125 rsmE 455250..455981 (-) 732 WP_068711938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAD080_RS02130 - 456067..456774 (-) 708 WP_342608033.1 endonuclease -
  AAD080_RS02135 - 456860..457360 (-) 501 WP_342608034.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41504.06 Da        Isoelectric Point: 5.2945

>NTDB_id=988217 AAD080_RS02100 WP_342608030.1 451733..452842(+) (pilU) [Vibrio tritonius strain JCM 16457 isolate MA35]
MFLEQCLSGMITRKASDVYITVGAPVLYRVNGELQGDGEKLTFSDVNNLLNRMMDSEQVEQFYHTHEANFAIVRDSGRFR
VSAFFQREQPGAVIRRIETRIPTFDELHLPELLKDLVMSKRGLVLVVGSTGSGKSTTMAAMTGYRNKNSSGHILTVEDPI
EFVHEHQGCIVTQREVGLDTDSYEVALKNSLRQAPDMILIGEIRSRETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKEQKDQFLFDLSMNLRGVVGQQLVRDSQGQGRYGAFEILLNTPRVSDLIRRGELHELKETMARSRESGMQTFDQALY
ELVIENKIAEEDAMHCADSANDLRLMLKTQRGDTSSGTGSLDHVTIDMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=988217 AAD080_RS02100 WP_342608030.1 451733..452842(+) (pilU) [Vibrio tritonius strain JCM 16457 isolate MA35]
ATGTTTCTGGAACAATGTTTAAGTGGAATGATTACTCGTAAAGCCTCTGATGTGTATATCACTGTCGGAGCACCTGTGTT
GTATCGAGTGAATGGTGAACTGCAAGGGGATGGTGAAAAACTCACATTTTCGGATGTGAATAATTTGCTTAACCGGATGA
TGGACAGTGAGCAAGTCGAGCAGTTTTACCATACCCATGAAGCAAACTTTGCGATTGTGCGCGATAGTGGGCGTTTTCGT
GTGAGTGCTTTTTTTCAACGCGAGCAACCCGGAGCAGTGATTCGCCGTATAGAAACGCGTATACCTACCTTTGATGAGCT
GCACTTACCTGAATTACTAAAAGATTTAGTGATGAGTAAGCGTGGTTTGGTACTGGTTGTTGGCTCTACGGGATCGGGTA
AATCAACAACGATGGCAGCCATGACTGGTTACCGTAATAAAAACTCATCTGGACATATTCTGACGGTGGAAGATCCGATT
GAATTTGTGCATGAACACCAAGGATGTATCGTGACTCAGCGTGAAGTGGGGCTAGATACAGATAGTTACGAAGTGGCACT
AAAAAATTCGCTTCGCCAAGCGCCTGATATGATTCTTATTGGTGAAATTCGTTCGCGTGAAACCATGGAATACGCTATGA
ATTTTGCCGAAACAGGTCATTTATGCATGGCAACGCTGCACGCCAACAATGCTAACCAAGCATTAGAGCGCATTTTACAT
CTGGTGCCTAAAGAGCAAAAAGATCAGTTTTTGTTTGATTTATCGATGAATTTGCGTGGTGTGGTTGGTCAACAATTGGT
TCGTGATAGCCAAGGGCAAGGACGTTACGGTGCGTTTGAGATTTTGCTAAATACACCGCGAGTGTCGGATTTGATTCGTC
GTGGAGAGCTCCACGAGCTGAAAGAAACTATGGCTCGATCTCGTGAATCGGGAATGCAGACCTTTGATCAAGCGTTGTAT
GAATTGGTCATTGAGAATAAGATCGCTGAAGAAGATGCAATGCACTGTGCTGATTCAGCCAATGATCTGCGCCTCATGCT
AAAAACCCAGCGAGGGGATACAAGCAGCGGAACCGGCTCTTTAGATCATGTCACCATTGATATGGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

76.965

100

0.77
  pilU Pseudomonas stutzeri DSM 10701

58

94.851

0.55
  pilU Acinetobacter baylyi ADP1

55.556

95.122

0.528
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.231

84.553

0.374
  pilT Legionella pneumophila strain Lp02

40.708

91.87

0.374
  pilT Legionella pneumophila strain ERS1305867

40.708

91.87

0.374
  pilT Acinetobacter baylyi ADP1

39.589

92.412

0.366
  pilT Acinetobacter baumannii D1279779

39.233

91.87

0.36
  pilT Acinetobacter baumannii strain A118

39.233

91.87

0.36
  pilT Acinetobacter nosocomialis M2

39.233

91.87

0.36
  pilT Pseudomonas aeruginosa PAK

39.701

90.786

0.36

Multiple sequence alignment