Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   V8V52_RS18735 Genome accession   NZ_CP150648
Coordinates   3662051..3662389 (-) Length   112 a.a.
NCBI ID   WP_088032755.1    Uniprot ID   A0A243AAR6
Organism   Bacillus mycoides strain 4BM1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3662051..3677355 3662051..3662389 within 0


Gene organization within MGE regions


Location: 3662051..3677355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V8V52_RS18735 (V8V52_18735) ssbA 3662051..3662389 (-) 339 WP_088032755.1 single-stranded DNA-binding protein Machinery gene
  V8V52_RS18740 (V8V52_18740) - 3662551..3662805 (-) 255 WP_088032753.1 DUF4318 domain-containing protein -
  V8V52_RS18745 (V8V52_18745) - 3662900..3663631 (-) 732 WP_088032751.1 Bax inhibitor-1/YccA family protein -
  V8V52_RS18750 (V8V52_18750) - 3663707..3665602 (-) 1896 WP_088032749.1 ABC-F family ATP-binding cassette domain-containing protein -
  V8V52_RS18755 (V8V52_18755) - 3665599..3666246 (-) 648 WP_088032748.1 HD domain-containing protein -
  V8V52_RS18760 (V8V52_18760) - 3666356..3667033 (+) 678 WP_088032747.1 hypothetical protein -
  V8V52_RS18765 (V8V52_18765) - 3667615..3667866 (-) 252 Protein_3645 hydrolase -
  V8V52_RS18770 (V8V52_18770) - 3667929..3668096 (-) 168 WP_084667385.1 hypothetical protein -
  V8V52_RS18775 (V8V52_18775) - 3668661..3669176 (-) 516 WP_088032745.1 DUF3231 family protein -
  V8V52_RS18780 (V8V52_18780) - 3669523..3669852 (+) 330 WP_002165182.1 WGxxGxxG family protein -
  V8V52_RS18785 (V8V52_18785) - 3670219..3670452 (-) 234 WP_002165181.1 hemolysin XhlA family protein -
  V8V52_RS18790 (V8V52_18790) - 3670953..3671495 (-) 543 WP_088032743.1 site-specific integrase -
  V8V52_RS18795 (V8V52_18795) - 3671492..3671962 (-) 471 Protein_3651 ArpU family phage packaging/lysis transcriptional regulator -
  V8V52_RS18800 (V8V52_18800) - 3673736..3674269 (+) 534 WP_328035304.1 hypothetical protein -
  V8V52_RS18805 (V8V52_18805) - 3674707..3675117 (-) 411 WP_088032739.1 hypothetical protein -
  V8V52_RS18810 (V8V52_18810) - 3675149..3675444 (-) 296 Protein_3654 hypothetical protein -
  V8V52_RS18815 (V8V52_18815) - 3675778..3675969 (-) 192 WP_044442009.1 DUF3954 domain-containing protein -
  V8V52_RS18820 (V8V52_18820) - 3676033..3676395 (-) 363 WP_088032735.1 cell division protein SepF -
  V8V52_RS18825 (V8V52_18825) - 3676380..3676535 (-) 156 WP_176280928.1 hypothetical protein -

Sequence


Protein


Download         Length: 112 a.a.        Molecular weight: 12968.76 Da        Isoelectric Point: 9.0926

>NTDB_id=970931 V8V52_RS18735 WP_088032755.1 3662051..3662389(-) (ssbA) [Bacillus mycoides strain 4BM1]
MMNRVVLIGRLTKEPELYYTKQGVAYARICVAVNRGFRNSLGEQQVDFINCVVWRKSAENVTEYCTKGSLVGITGRIHTS
NYDDEQGKRVYRTEVLIENITFLERRRESTSQ

Nucleotide


Download         Length: 339 bp        

>NTDB_id=970931 V8V52_RS18735 WP_088032755.1 3662051..3662389(-) (ssbA) [Bacillus mycoides strain 4BM1]
ATGATGAATCGAGTTGTATTAATCGGTAGATTGACAAAGGAGCCAGAATTATACTACACAAAACAAGGTGTCGCTTATGC
ACGAATATGTGTTGCGGTGAATAGAGGTTTTCGAAATAGTTTAGGTGAACAACAAGTAGATTTTATAAATTGTGTCGTAT
GGCGAAAATCAGCTGAAAATGTAACAGAATATTGTACGAAGGGTTCACTTGTTGGGATTACAGGGCGTATTCATACGAGT
AATTACGATGATGAACAAGGAAAGAGAGTATATAGAACTGAAGTTCTGATTGAGAATATTACATTTTTAGAGAGAAGGCG
GGAGAGTACATCGCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A243AAR6

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

56.604

94.643

0.536

  ssb Latilactobacillus sakei subsp. sakei 23K

54.717

94.643

0.518

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.887

94.643

0.491

  ssbB Streptococcus sobrinus strain NIDR 6715-7

44.34

94.643

0.42

  ssbB Lactococcus lactis subsp. cremoris KW2

44.762

93.75

0.42

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.453

94.643

0.402

  ssbB/cilA Streptococcus mitis NCTC 12261

42.453

94.643

0.402

  ssbB/cilA Streptococcus pneumoniae Rx1

41.509

94.643

0.393

  ssbB/cilA Streptococcus pneumoniae D39

41.509

94.643

0.393

  ssbB/cilA Streptococcus pneumoniae R6

41.509

94.643

0.393

  ssbB/cilA Streptococcus mitis SK321

41.509

94.643

0.393

  ssbA Streptococcus mutans UA159

40.566

94.643

0.384


Multiple sequence alignment