Detailed information NTG0962377

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: WKQ99_RS15555
• Genome accession: NZ_CP149965
• Coordinates: 3393935..3395044 (-)
• Length: 369 a.a.
• NCBI ID: WP_323167354.1
• Uniprot ID: -
• Organism: Pseudomonas atacamensis strain EMP42
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3388935..3400044

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
WKQ99_RS15550 (WKQ99_15550)	-	3390042..3393800 (-)	3759	WP_339099981.1	hypothetical protein	-
WKQ99_RS15555 (WKQ99_15555)	pilU	3393935..3395044 (-)	1110	WP_323167354.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
WKQ99_RS15560 (WKQ99_15560)	-	3395154..3397703 (+)	2550	WP_339099982.1	PAS domain-containing protein	-
WKQ99_RS15565 (WKQ99_15565)	-	3397744..3398019 (+)	276	WP_007912322.1	peptidylprolyl isomerase	-
WKQ99_RS15570 (WKQ99_15570)	-	3398136..3399056 (-)	921	WP_339099983.1	sugar kinase	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40771.63 Da
• Isoelectric Point: 7.0374

>NTDB_id=962377 WKQ99_RS15555 WP_323167354.1 3393935..3395044(-) (pilU) [Pseudomonas atacamensis strain EMP42]
MEIDALLSTLSQKNGSDLFLSTGAAPSARIDGALTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKVDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKACVSQRLLRTVDGQRRAAVEILLGTPTIADLVRRGQFEELKPMMEKSTGLGMQTFDAAL
FALFAEGAISEQEALKNADSVNNLKLRIKLQHDQAGVNNTGAGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=962377 WKQ99_RS15555 WP_323167354.1 3393935..3395044(-) (pilU) [Pseudomonas atacamensis strain EMP42]
ATGGAAATCGATGCACTGTTGTCAACATTGTCGCAAAAAAACGGCTCGGATCTGTTCCTCTCTACCGGCGCAGCGCCGAG
TGCGCGCATTGATGGCGCGCTGACGGCGCTCAGCGATCAGCCATTCAAACCCGGCGAAACCGCGGCTATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCAATCTCCCGAACGGGCGTCGGGCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTTTCGATCGTGATCCGCAACGTCAAGGTCGACATCCCGCGATTCGA
AGACCTGAAGCTGCCGCCGGTGTTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCGACCAGTT
CAGGCAAGTCGACTTCACTGGCGGCACTCATCGATCACCGTAACCGCCACAGTGCCGGACACATCGTGACGATCGAAGAC
CCGATCGAATATATCCATCGGCACAAGCGCTCGATCATCAATCAGCGTGAAGTCGGCGTAGATACCCGAAGCTTTCATGC
GGCTTTGAAGAACACTCTGCGCCAGGCTCCGGATGTTGTATTGATTGGTGAGATCCGCGATCGCGAAACCATGGAGCATG
CGCTGTCATTCGCTGATACCGGCCATCTTGTTTTGTCGACGCTGCATGCGACCAATGCCAATCAGGCACTGGATCGAATC
ATCAATATGTTCCCCGAAGAGAAGCGCGAGCAGCTTTTGCAGACATTGGGCAACAACCTCAAAGCCTGTGTTTCGCAGCG
TCTGCTCCGCACTGTCGATGGGCAAAGGCGCGCGGCAGTCGAAATTCTTCTGGGTACGCCGACCATCGCTGACCTGGTCC
GACGCGGCCAGTTCGAAGAGCTCAAGCCGATGATGGAAAAATCGACCGGACTCGGTATGCAGACATTTGACGCGGCACTG
TTTGCGCTGTTTGCAGAAGGTGCAATCAGTGAGCAGGAAGCATTGAAGAATGCCGATTCGGTGAATAACTTGAAGTTGCG
AATCAAACTTCAGCACGATCAGGCAGGTGTTAACAATACAGGCGCCGGCGAGTGGGGTTTGATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.622	96.748	0.528
pilU	Acinetobacter baylyi ADP1	53.125	95.393	0.507
pilU	Vibrio cholerae strain A1552	51.143	94.851	0.485
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.593	91.87	0.382
pilT	Pseudomonas stutzeri DSM 10701	39.826	93.225	0.371
pilT	Vibrio cholerae strain A1552	40.541	90.244	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.541	90.244	0.366
pilT	Acinetobacter baumannii D1279779	40	90.786	0.363
pilT	Acinetobacter baumannii strain A118	40	90.786	0.363
pilT	Pseudomonas aeruginosa PAK	38.953	93.225	0.363
pilT	Acinetobacter nosocomialis M2	39.701	90.786	0.36