Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   WJM57_RS08325 Genome accession   NZ_CP149127
Coordinates   1712334..1713473 (-) Length   379 a.a.
NCBI ID   WP_011898593.1    Uniprot ID   A2T1B1
Organism   Aeromonas salmonicida strain BE4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1707334..1718473
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJM57_RS08300 feoB 1707537..1709807 (+) 2271 WP_339023431.1 Fe(2+) transporter permease subunit FeoB -
  WJM57_RS08305 - 1709804..1710046 (+) 243 WP_021140137.1 FeoC-like transcriptional regulator -
  WJM57_RS08310 - 1710161..1710640 (-) 480 WP_339023432.1 WbuC family cupin fold metalloprotein -
  WJM57_RS08315 glgC 1710787..1712004 (-) 1218 WP_339023433.1 glucose-1-phosphate adenylyltransferase -
  WJM57_RS08320 - 1711916..1712356 (-) 441 WP_339023434.1 hypothetical protein -
  WJM57_RS08325 pilU 1712334..1713473 (-) 1140 WP_011898593.1 type IVa pilus ATPase TapW Machinery gene
  WJM57_RS08330 - 1713533..1713889 (-) 357 WP_005314602.1 DMT family protein -
  WJM57_RS08335 - 1714001..1714732 (+) 732 WP_058394151.1 transporter substrate-binding domain-containing protein -
  WJM57_RS08340 btuC 1714836..1715843 (+) 1008 WP_058394150.1 vitamin B12 ABC transporter permease BtuC -
  WJM57_RS08345 - 1715903..1716712 (+) 810 WP_181455208.1 ABC transporter ATP-binding protein -
  WJM57_RS08350 aac(6') 1716735..1717193 (-) 459 WP_125601800.1 aminoglycoside 6'-N-acetyltransferase -
  WJM57_RS08355 - 1717201..1718265 (-) 1065 WP_339023435.1 3-deoxy-7-phosphoheptulonate synthase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42265.26 Da        Isoelectric Point: 6.4288

>NTDB_id=956712 WJM57_RS08325 WP_011898593.1 1712334..1713473(-) (pilU) [Aeromonas salmonicida strain BE4]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETSLEPGEVAKIAADIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIANSADATGWSLEPTKDESNDPF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=956712 WJM57_RS08325 WP_011898593.1 1712334..1713473(-) (pilU) [Aeromonas salmonicida strain BE4]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
TGCCAAGTTCAACGGTGGGCTGCGCCCCCTGTCCGAGACGTCGCTCGAACCGGGTGAAGTAGCCAAGATCGCCGCCGACA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCCCGCAACATCAAGACAGAGATCCCCCGCTTTGA
GGATCTCAAACTGCCGCCCGTGCTGCTCGACACCATAATGGAGAAACGGGGCCTGGTGCTGTTTGTGGGCGGAACAGGCT
CGGGCAAATCCACGTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGCAGCTTCCATGC
CGCGCTCAAGAACACCCTGCGCCAGGCACCGGATGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAACATG
CCCTTGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAACTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACAGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGTGAGTTTGGCAGCCTGAAAGAGGTGATGGAGAAATCCAGAGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAAGCGGTGAAAAACGCCGACTCGGCCAACAACCTTCGCCTCAA
GATCAAGCTGTGGAAAGAGAAGGGCCAGATCGCCAACAGTGCGGATGCCACCGGCTGGAGTCTGGAGCCCACCAAGGATG
AAAGTAACGATCCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2T1B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.158

100

0.533
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.476

99.736

0.404
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361