Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   WAX86_RS13040 Genome accession   NZ_CP146970
Coordinates   2857934..2858329 (+) Length   131 a.a.
NCBI ID   WP_138241975.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain WMD-P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2852934..2863329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAX86_RS13025 (WAX86_13025) aceE 2853027..2855687 (-) 2661 WP_044174350.1 pyruvate dehydrogenase (acetyl-transferring), homodimeric type -
  WAX86_RS13030 (WAX86_13030) pdhR 2855768..2856541 (-) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WAX86_RS13035 (WAX86_13035) ampD 2856901..2857479 (-) 579 WP_086367527.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WAX86_RS13040 (WAX86_13040) pilA 2857934..2858329 (+) 396 WP_138241975.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  WAX86_RS13045 (WAX86_13045) pilB 2858329..2860020 (+) 1692 WP_341488889.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WAX86_RS13050 (WAX86_13050) pilC 2860086..2861312 (+) 1227 WP_341488890.1 type II secretion system F family protein Machinery gene
  WAX86_RS13055 (WAX86_13055) pilD 2861495..2862373 (+) 879 WP_065170821.1 A24 family peptidase Machinery gene
  WAX86_RS13060 (WAX86_13060) coaE 2862375..2862977 (+) 603 WP_341488891.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 131 a.a.        Molecular weight: 13228.05 Da        Isoelectric Point: 5.0178

>NTDB_id=945218 WAX86_RS13040 WP_138241975.1 2857934..2858329(+) (pilA) [Photobacterium damselae subsp. damselae strain WMD-P2]
MKGQKGFTLIELMIVVAVIGVLSAIAIPKYQQYVEKGALASALATATALKTNYEDYIAVSGAAPTTSAAIGATNFELGTV
TVTSGAIEVIVTKGGGSGSGVTLERSSDSWSCEVSASSDTIKLNGCTTKSS

Nucleotide


Download         Length: 396 bp        

>NTDB_id=945218 WAX86_RS13040 WP_138241975.1 2857934..2858329(+) (pilA) [Photobacterium damselae subsp. damselae strain WMD-P2]
ATGAAAGGACAAAAGGGTTTTACCTTAATTGAATTGATGATCGTAGTGGCGGTCATTGGCGTGTTATCTGCGATTGCTAT
ACCTAAGTACCAACAATACGTAGAAAAAGGAGCATTAGCCTCTGCATTAGCTACTGCTACTGCATTAAAAACTAATTATG
AAGATTATATTGCTGTTAGCGGTGCAGCACCAACAACATCCGCTGCTATTGGCGCAACAAATTTTGAACTAGGTACAGTG
ACAGTCACATCTGGAGCTATTGAAGTTATAGTAACAAAAGGAGGTGGTTCAGGTAGTGGAGTAACATTAGAAAGATCTTC
TGATTCATGGTCCTGTGAAGTAAGTGCTTCAAGTGACACCATTAAATTGAATGGTTGTACTACAAAGTCAAGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(39-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

47.887

100

0.519
  pilA Vibrio cholerae strain A1552

47.887

100

0.519
  pilA Vibrio cholerae C6706

47.887

100

0.519
  pilA Vibrio parahaemolyticus RIMD 2210633

41.912

100

0.435
  pilA Acinetobacter baumannii strain A118

40.288

100

0.427
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.443

93.13

0.405
  comP Acinetobacter baylyi ADP1

37.121

100

0.374
  pilA/pilAII Pseudomonas stutzeri DSM 10701

40

91.603

0.366