Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WAX86_RS13045 Genome accession   NZ_CP146970
Coordinates   2858329..2860020 (+) Length   563 a.a.
NCBI ID   WP_341488889.1    Uniprot ID   -
Organism   Photobacterium damselae subsp. damselae strain WMD-P2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2853329..2865020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WAX86_RS13030 (WAX86_13030) pdhR 2855768..2856541 (-) 774 WP_005297609.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  WAX86_RS13035 (WAX86_13035) ampD 2856901..2857479 (-) 579 WP_086367527.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  WAX86_RS13040 (WAX86_13040) pilA 2857934..2858329 (+) 396 WP_138241975.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  WAX86_RS13045 (WAX86_13045) pilB 2858329..2860020 (+) 1692 WP_341488889.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WAX86_RS13050 (WAX86_13050) pilC 2860086..2861312 (+) 1227 WP_341488890.1 type II secretion system F family protein Machinery gene
  WAX86_RS13055 (WAX86_13055) pilD 2861495..2862373 (+) 879 WP_065170821.1 A24 family peptidase Machinery gene
  WAX86_RS13060 (WAX86_13060) coaE 2862375..2862977 (+) 603 WP_341488891.1 dephospho-CoA kinase -
  WAX86_RS13065 (WAX86_13065) zapD 2863045..2863782 (+) 738 WP_005297594.1 cell division protein ZapD -
  WAX86_RS13070 (WAX86_13070) yacG 2863791..2864000 (+) 210 WP_005297592.1 DNA gyrase inhibitor YacG -
  WAX86_RS13075 (WAX86_13075) mutT 2864067..2864474 (+) 408 WP_188007686.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 62571.18 Da        Isoelectric Point: 5.4773

>NTDB_id=945219 WAX86_RS13045 WP_341488889.1 2858329..2860020(+) (pilB) [Photobacterium damselae subsp. damselae strain WMD-P2]
MHTTLASILHQADLISSVQQQQVEDSVSMEGITVPSALIHLGIFSDRELAQHLSHIFGLTLVSVHDYDFEACCKAVYQRD
LVLRHRALPLYQSANTLFLGVSDPCNIDGQDEFRFATGRNIEPLLLENKQLESAIRRIYGRELGETTNMASRDVSDDDLN
NLVDIGDEERDQATDLGQENEPVTRYINQVLLDAVRKKASDIHFEPYETSYRIRFRCDGILHQHATPPAHLSRRLSTRLK
VMSRLNIAERRQPQDGRIKLKLTANLAVDLRVSTLPTLWGEKVVLRILDSSSANLDIDILGYNQQQKAAYLNALQQPQGM
ILMTGPTGSGKTVSLYTGLSILNTEERNISTAEDPVEINLPGINQVHINNQAGLGFAEALRSFLRQDPDVVMVGEIRDLE
TASIAVKAAQTGHLVLSTLHTNSAAETITRLTNMGIEDFNLASSLTLIIAQRLGRRLCPHCKQADEIEGFMQDKLRIPAG
TTLYKANPEGCDDCNKGYLGRVGIYEVMPFSRRLAQALVDGATTLELENIAVEEGMLTLQQSGIEKLCLGITSLKELQRV
LHF

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=945219 WAX86_RS13045 WP_341488889.1 2858329..2860020(+) (pilB) [Photobacterium damselae subsp. damselae strain WMD-P2]
ATGCACACCACTCTCGCCTCGATTCTGCACCAGGCTGATCTGATCAGCTCGGTGCAGCAGCAACAAGTCGAAGACAGTGT
CAGTATGGAGGGGATCACGGTTCCCTCCGCACTGATCCATCTGGGTATATTTAGCGATCGCGAACTGGCCCAACACCTTA
GCCACATTTTTGGTCTCACCCTCGTTTCAGTGCATGACTACGACTTTGAAGCTTGCTGTAAAGCGGTCTATCAACGCGAT
TTGGTATTACGTCATCGCGCGCTACCGTTATACCAAAGCGCCAATACGTTATTTTTAGGGGTCAGCGATCCATGCAATAT
CGACGGACAAGATGAGTTCCGTTTTGCCACTGGACGCAATATTGAACCACTACTGCTGGAAAATAAGCAGCTCGAATCGG
CTATCCGTCGTATTTATGGTCGTGAGTTGGGCGAAACCACCAATATGGCCAGTCGTGACGTCAGTGACGATGATCTAAAT
AACTTAGTCGATATTGGTGATGAAGAGCGGGATCAAGCTACAGATCTAGGTCAAGAAAACGAACCTGTTACTCGCTATAT
CAATCAAGTGCTGCTCGATGCGGTACGTAAAAAAGCCTCCGATATCCATTTTGAACCTTATGAGACCAGTTATCGGATCC
GCTTTCGTTGTGATGGCATCTTACACCAACACGCAACACCACCAGCCCATCTTTCTCGTCGTCTCTCTACCCGACTCAAA
GTGATGTCGCGCCTAAATATTGCCGAACGACGCCAACCGCAAGATGGACGCATAAAACTCAAGCTCACAGCCAATTTAGC
CGTAGACCTCCGTGTTTCTACCCTGCCCACTTTATGGGGTGAAAAAGTGGTGCTGCGTATTTTAGATAGCTCCAGTGCCA
ACTTAGACATTGATATTTTAGGTTACAACCAACAACAAAAAGCCGCTTATCTTAATGCTCTGCAACAACCGCAAGGGATG
ATCTTAATGACAGGGCCTACGGGTAGCGGCAAAACCGTTTCGCTTTATACCGGCCTTAGCATTTTAAATACCGAAGAGCG
CAATATTTCTACCGCAGAAGATCCGGTAGAGATCAACTTACCTGGCATTAATCAGGTTCACATTAACAATCAAGCAGGAC
TGGGGTTTGCTGAAGCGCTGCGCTCATTTTTACGTCAAGACCCTGATGTAGTGATGGTAGGTGAAATCCGCGATCTGGAA
ACCGCCTCTATAGCCGTTAAAGCAGCGCAAACTGGACATTTAGTACTATCGACCTTGCACACCAATTCTGCTGCCGAAAC
CATCACCCGCTTAACCAATATGGGTATTGAAGACTTTAACCTAGCCTCATCACTGACACTGATTATTGCTCAGCGCTTAG
GTCGCCGGCTCTGCCCTCACTGTAAACAAGCAGATGAAATTGAGGGCTTTATGCAAGACAAACTGCGCATTCCAGCTGGC
ACTACCTTGTATAAAGCCAATCCAGAAGGGTGTGATGACTGCAATAAAGGCTATTTAGGTCGTGTCGGTATCTATGAGGT
GATGCCGTTTAGTCGCCGTCTTGCTCAAGCTCTGGTTGATGGTGCTACCACCTTAGAGCTGGAAAATATCGCAGTAGAAG
AAGGGATGCTAACGCTACAACAATCGGGAATAGAAAAGCTCTGTCTTGGTATCACCAGTTTAAAAGAATTACAGCGCGTC
CTGCATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

61.812

100

0.618
  pilB Vibrio parahaemolyticus RIMD 2210633

60.924

100

0.609
  pilB Vibrio campbellii strain DS40M4

60.391

100

0.604
  pilB Legionella pneumophila strain ERS1305867

50

96.27

0.481
  pilB Acinetobacter baylyi ADP1

48.905

97.336

0.476
  pilB Acinetobacter baumannii D1279779

50.958

92.718

0.472
  pilF Neisseria gonorrhoeae MS11

44.464

100

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

39.692

92.185

0.366
  pilB Haemophilus influenzae 86-028NP

41.856

86.146

0.361
  pilB Haemophilus influenzae Rd KW20

41.856

86.146

0.361