NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	VY732_RS13185	Genome accession	NZ_CP144274
Coordinates	2967447..2968556 (+)	Length	369 a.a.
NCBI ID	WP_331695633.1	Uniprot ID	-
Organism	Pseudomonas sp. ZY71
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2962447..2973556

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VY732_RS13170 (VY732_13170)	-	2963422..2964360 (+)	939	WP_331695631.1	sugar kinase	-
VY732_RS13175 (VY732_13175)	-	2964469..2964744 (-)	276	WP_007912322.1	peptidylprolyl isomerase	-
VY732_RS13180 (VY732_13180)	-	2964796..2967333 (-)	2538	WP_331695632.1	PAS domain S-box protein	-
VY732_RS13185 (VY732_13185)	pilU	2967447..2968556 (+)	1110	WP_331695633.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
VY732_RS13190 (VY732_13190)	-	2968690..2972457 (+)	3768	WP_147304404.1	hypothetical protein	-

Sequence

Protein

Download Length: 369 a.a. Molecular weight: 40677.50 Da Isoelectric Point: 6.8443

                                >NTDB_id=933227 VY732_RS13185 WP_331695633.1 2967447..2968556(+) (pilU) [Pseudomonas sp. ZY71]

MEIDALLSVLSNKNGSDLFLSTGAPPSARIDGVLTALSEQPFKNGETAAIAASLMDVEQRREFDRDLEMNLAISRAGIGR

FRVNIFKQRNDVSIVIRNVKLDIPRFADLKLPAVLLDTVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED

PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHATNANQALDRI

INMFPEERRPQLLQTLGNNLKAFISQRLVRTVDGQRRAAVEVLLGTPTIADLIRHGQFDELKPIMEKSTEPGMQTFDAAL

FSLVAEGAISARDALKNADSMNNLKLRIKLAGDESSNKGSPSGEWGLMD

Nucleotide

Download Length: 1110 bp

                                >NTDB_id=933227 VY732_RS13185 WP_331695633.1 2967447..2968556(+) (pilU) [Pseudomonas sp. ZY71]

ATGGAAATCGATGCACTGTTGTCAGTTTTGTCCAATAAAAACGGTTCGGATCTGTTCCTGTCTACAGGAGCACCGCCCAG

TGCGCGTATCGATGGCGTGCTCACGGCGTTGAGCGAACAGCCTTTCAAGAACGGTGAGACCGCTGCCATCGCCGCCTCAC

TGATGGACGTCGAGCAACGACGGGAGTTCGACCGGGATCTGGAAATGAATCTGGCGATTTCGCGAGCGGGCATTGGTCGC

TTTCGCGTGAACATCTTCAAGCAGCGCAACGATGTATCGATCGTCATTCGTAACGTCAAACTCGATATTCCGCGTTTCGC

CGATCTGAAACTGCCGGCGGTACTGCTCGACACCGTCATGCTCAAGCAAGGGTTGATCCTCTTTGTTGGCGCCACCGACT

CGGGGAAATCAACCTCGCTCGCCGCGCTGATCGATCATCGCAACCGCCACAGTAGTGGGCACATCATCACTATTGAGGAC

CCGATCGAATACATCCATCGGCATCAGCGCTCGATCATCAATCAACGTGAAGTCGGAGTCGATACACGCAGCTTTCACGC

CGCGTTGAAAAACACCCTGCGCCAAGCCCCGGACGTGGTGCTGATCGGCGAAATTCGTGACCGCGAAACCATGGAGCATG

CGTTGGCATTTGCCGATACCGGGCACCTGGTGTTGTCGACGTTGCATGCCACCAACGCCAATCAGGCGCTGGACCGGATC

ATCAATATGTTCCCGGAAGAGCGGCGGCCGCAGTTGTTGCAGACGCTGGGCAATAACCTGAAAGCATTTATCTCGCAGCG

GTTGGTGCGCACTGTCGACGGGCAACGACGGGCTGCGGTCGAGGTGCTGTTGGGCACGCCAACCATCGCTGATCTGATCC

GACACGGTCAGTTCGATGAACTCAAGCCGATCATGGAGAAATCCACCGAACCGGGTATGCAGACATTTGATGCAGCGCTG

TTTTCGTTGGTTGCCGAAGGTGCAATCAGTGCACGGGATGCTTTGAAAAATGCAGACTCGATGAATAATCTTAAATTGCG

AATTAAGCTGGCTGGAGATGAAAGTAGTAACAAGGGTTCGCCGTCAGGGGAGTGGGGACTAATGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-271)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Pseudomonas stutzeri DSM 10701	55.707	99.729	0.556
pilU	Acinetobacter baylyi ADP1	53.889	97.561	0.526
pilU	Vibrio cholerae strain A1552	50.139	97.832	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.945	98.374	0.393
pilT	Acinetobacter baumannii strain A118	39.42	93.496	0.369
pilT	Acinetobacter baumannii D1279779	39.42	93.496	0.369
pilT	Acinetobacter nosocomialis M2	39.13	93.496	0.366
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.24	90.244	0.363
pilT	Vibrio cholerae strain A1552	40.24	90.244	0.363
pilT	Acinetobacter baylyi ADP1	38.663	93.225	0.36
pilT	Pseudomonas stutzeri DSM 10701	38.663	93.225	0.36

Detailed information NTG0933227