Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VUI23_RS16130 Genome accession   NZ_CP144164
Coordinates   3764543..3764953 (+) Length   136 a.a.
NCBI ID   WP_342805003.1    Uniprot ID   -
Organism   Alteromonas sp. M12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3759543..3769953
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUI23_RS16105 pdhR 3760340..3761095 (-) 756 WP_216047208.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VUI23_RS16110 ampE 3761383..3762207 (-) 825 WP_303501359.1 beta-lactamase regulator AmpE -
  VUI23_RS16115 ampD 3762280..3762855 (-) 576 WP_342805001.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VUI23_RS16120 - 3762913..3763437 (+) 525 WP_216047212.1 TIGR02281 family clan AA aspartic protease -
  VUI23_RS16125 nadC 3763498..3764337 (+) 840 WP_342805002.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VUI23_RS16130 pilA 3764543..3764953 (+) 411 WP_342805003.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VUI23_RS16135 pilC 3765035..3766243 (+) 1209 WP_342805004.1 type II secretion system F family protein Machinery gene
  VUI23_RS16140 pilD 3766361..3767239 (+) 879 WP_342805005.1 A24 family peptidase Machinery gene
  VUI23_RS16145 coaE 3767236..3767853 (+) 618 WP_216047217.1 dephospho-CoA kinase -
  VUI23_RS16150 zapD 3768139..3768891 (+) 753 WP_303501372.1 cell division protein ZapD -
  VUI23_RS16155 yacG 3768907..3769140 (+) 234 WP_303501375.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13889.88 Da        Isoelectric Point: 4.6320

>NTDB_id=932554 VUI23_RS16130 WP_342805003.1 3764543..3764953(+) (pilA) [Alteromonas sp. M12]
MKMTQMTNNKGFTLIELMIVVAIIGILAAIALPAYQGYTQKAKFTEVTNATAAAKSAVEVCAQVNNALTDCDGGSNGIPA
NVTVSAAEVGLTTTDGTIVTEGKTGSDLAGKTYTLTAAYAAGKVTWTPTCSDTTLC

Nucleotide


Download         Length: 411 bp        

>NTDB_id=932554 VUI23_RS16130 WP_342805003.1 3764543..3764953(+) (pilA) [Alteromonas sp. M12]
ATGAAAATGACTCAAATGACTAACAACAAAGGTTTTACACTAATTGAATTGATGATCGTTGTAGCGATTATCGGTATTCT
TGCAGCCATCGCATTACCTGCTTACCAAGGATACACGCAAAAAGCGAAGTTTACTGAAGTAACTAATGCGACAGCAGCAG
CGAAATCAGCGGTTGAAGTATGTGCACAGGTTAACAATGCATTGACTGATTGTGACGGTGGTTCAAATGGTATCCCAGCT
AACGTAACAGTATCTGCAGCAGAAGTAGGTTTGACTACTACTGACGGTACTATCGTAACTGAAGGTAAGACTGGTAGTGA
TTTGGCAGGTAAAACCTATACTTTAACTGCTGCATATGCGGCTGGCAAGGTAACTTGGACTCCAACTTGTAGTGATACTA
CTCTTTGTTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(41-127)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

50

100

0.5
  pilA Haemophilus influenzae Rd KW20

47.794

100

0.478
  pilA Vibrio campbellii strain DS40M4

43.448

100

0.463
  comP Acinetobacter baylyi ADP1

41.667

97.059

0.404
  pilA2 Legionella pneumophila str. Paris

42.188

94.118

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.491

83.824

0.39
  pilA Glaesserella parasuis strain SC1401

42.063

92.647

0.39
  pilA Pseudomonas aeruginosa PAK

37.405

96.324

0.36
  pilA Vibrio parahaemolyticus RIMD 2210633

39.516

91.176

0.36