Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   VUI23_RS16135 Genome accession   NZ_CP144164
Coordinates   3765035..3766243 (+) Length   402 a.a.
NCBI ID   WP_342805004.1    Uniprot ID   -
Organism   Alteromonas sp. M12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3760035..3771243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VUI23_RS16105 pdhR 3760340..3761095 (-) 756 WP_216047208.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VUI23_RS16110 ampE 3761383..3762207 (-) 825 WP_303501359.1 beta-lactamase regulator AmpE -
  VUI23_RS16115 ampD 3762280..3762855 (-) 576 WP_342805001.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VUI23_RS16120 - 3762913..3763437 (+) 525 WP_216047212.1 TIGR02281 family clan AA aspartic protease -
  VUI23_RS16125 nadC 3763498..3764337 (+) 840 WP_342805002.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VUI23_RS16130 pilA 3764543..3764953 (+) 411 WP_342805003.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  VUI23_RS16135 pilC 3765035..3766243 (+) 1209 WP_342805004.1 type II secretion system F family protein Machinery gene
  VUI23_RS16140 pilD 3766361..3767239 (+) 879 WP_342805005.1 A24 family peptidase Machinery gene
  VUI23_RS16145 coaE 3767236..3767853 (+) 618 WP_216047217.1 dephospho-CoA kinase -
  VUI23_RS16150 zapD 3768139..3768891 (+) 753 WP_303501372.1 cell division protein ZapD -
  VUI23_RS16155 yacG 3768907..3769140 (+) 234 WP_303501375.1 DNA gyrase inhibitor YacG -
  VUI23_RS16160 recJ 3769130..3770857 (-) 1728 WP_342805006.1 single-stranded-DNA-specific exonuclease RecJ -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43504.39 Da        Isoelectric Point: 9.3910

>NTDB_id=932555 VUI23_RS16135 WP_342805004.1 3765035..3766243(+) (pilC) [Alteromonas sp. M12]
MAKTAEMYTWVGVDKKGNSRKGEISAISLSEAKNMLRRQGVSATKVKKLAKPLFGRKDKVSAQDICVLSRQIATMLGAGV
TLIQTIDMIAQGHAKQSMRRMLTEIANEVKAGNPLSSALRKHPDQFEDLYCDLVETGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVVVVAFIVTTILLIFVVPQFQEIFSSFGAELPAFTLFVLAISQFVQDFGIFIAAGTAIAAVLFMRAH
KKSKNLQDKVDMLILKIPIIGEILKKASIARFTRTLATTFAAGVPLIGALDSAAGASGNAVFRDAILFIKREVAGGMQMN
TAMRATGVFPDMVTQMIAIGEESGAVDDMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIIAMYLPIFQMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=932555 VUI23_RS16135 WP_342805004.1 3765035..3766243(+) (pilC) [Alteromonas sp. M12]
ATGGCTAAAACAGCAGAAATGTATACCTGGGTTGGAGTCGATAAAAAAGGCAACAGTCGTAAAGGCGAGATCAGTGCTAT
TTCGTTGTCGGAAGCGAAAAACATGCTACGCCGCCAAGGTGTTTCTGCCACTAAAGTTAAGAAACTCGCCAAGCCACTAT
TTGGTAGAAAAGACAAAGTTTCCGCTCAAGATATTTGTGTGTTATCTCGACAAATCGCCACCATGCTTGGGGCGGGTGTT
ACATTAATTCAAACCATCGACATGATCGCCCAAGGTCATGCAAAACAAAGCATGCGCCGAATGCTTACCGAGATTGCCAA
TGAAGTCAAAGCCGGCAATCCGCTATCAAGTGCGCTGCGCAAACATCCTGATCAATTTGAAGACCTATATTGTGATTTGG
TTGAAACCGGTGAACAATCCGGTGCCTTAGAAACAATCTATGATCGAATCGCGACTTATAAAGAAAAAGCCGAAGCGTTA
AAATCGAAAATCAAAAAAGCCATGTTCTATCCAATTGCGGTAGTAGTAGTCGCCTTTATTGTAACCACTATCCTATTGAT
ATTTGTAGTACCGCAGTTTCAAGAAATATTCAGTAGCTTTGGAGCTGAATTACCCGCCTTTACCTTATTTGTATTAGCTA
TTTCCCAATTTGTGCAAGATTTTGGCATTTTCATCGCGGCGGGCACAGCGATTGCTGCGGTTTTATTTATGCGGGCCCAC
AAAAAGTCAAAAAACTTACAAGATAAAGTGGATATGCTAATTCTCAAAATACCCATTATTGGTGAAATTTTAAAAAAAGC
CTCAATCGCTCGATTTACCCGTACACTTGCAACCACCTTTGCTGCTGGTGTGCCTTTGATTGGCGCATTGGACTCAGCCG
CTGGCGCTTCGGGCAATGCCGTTTTTCGGGATGCTATATTATTTATTAAGCGGGAAGTTGCTGGCGGTATGCAAATGAAC
ACCGCGATGCGCGCAACAGGCGTGTTTCCAGACATGGTGACGCAAATGATTGCCATCGGTGAAGAGTCTGGTGCAGTGGA
TGATATGCTCAGTAAAATTGCAACTATTTACGAAGCCGAAGTAGATGACATGGTTGACGGCTTAACCAGTTTGTTAGAGC
CGATGATTATGGCCGTTTTAGGGGTGGTCATTGGTGGTTTGATTATCGCTATGTATTTACCAATCTTCCAAATGGGTAAC
GTGGTTTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.586

98.507

0.577
  pilC Acinetobacter baumannii D1279779

51.852

100

0.522
  pilC Acinetobacter baylyi ADP1

51.75

99.502

0.515
  pilC Legionella pneumophila strain ERS1305867

49.246

99.005

0.488
  pilC Vibrio cholerae strain A1552

46.212

98.507

0.455
  pilC Vibrio campbellii strain DS40M4

43

99.502

0.428
  pilG Neisseria gonorrhoeae MS11

43.75

95.522

0.418
  pilG Neisseria meningitidis 44/76-A

42.932

95.025

0.408