Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   V0M98_RS13715 Genome accession   NZ_CP143487
Coordinates   2987438..2988547 (+) Length   369 a.a.
NCBI ID   WP_338923098.1    Uniprot ID   -
Organism   Pseudomonas silesiensis strain G3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982438..2993547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V0M98_RS13690 (V0M98_13690) - 2982447..2982836 (+) 390 WP_338923095.1 RidA family protein -
  V0M98_RS13695 (V0M98_13695) - 2982842..2982964 (+) 123 Protein_2682 IclR family transcriptional regulator -
  V0M98_RS13700 (V0M98_13700) - 2983004..2984221 (+) 1218 WP_338923096.1 amino acid deaminase -
  V0M98_RS13705 (V0M98_13705) - 2984439..2984714 (-) 276 WP_028622750.1 peptidylprolyl isomerase -
  V0M98_RS13710 (V0M98_13710) - 2984781..2987318 (-) 2538 WP_338923097.1 PAS domain-containing protein -
  V0M98_RS13715 (V0M98_13715) pilU 2987438..2988547 (+) 1110 WP_338923098.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  V0M98_RS13720 (V0M98_13720) - 2988634..2990589 (-) 1956 WP_338923099.1 acetoacetate--CoA ligase -
  V0M98_RS13725 (V0M98_13725) - 2990774..2991559 (-) 786 WP_338923100.1 3-hydroxybutyrate dehydrogenase -
  V0M98_RS13730 (V0M98_13730) - 2991567..2992958 (-) 1392 WP_338923101.1 GntP family permease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40855.84 Da        Isoelectric Point: 6.3917

>NTDB_id=929155 V0M98_RS13715 WP_338923098.1 2987438..2988547(+) (pilU) [Pseudomonas silesiensis strain G3]
MEIDALLKVLAHQDGSDLYLSTGAPPCARFDGVLKPLADQPFKPGEIAAIAASIMDAEQRLEFDRELEMNLAISLAGIGR
FRVNIFKQRNDVSIVVRNVKLDIPRFEDLKLPSVLLEAVMLKQGLMLFVGATGSGKSTSLAALIDYRNRHSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRI
INFFPEERRAQLLNDLGNNLKAFVSQRLVPTRDGHRRAAVEVMLGSPTIGDLIRRNEFAELKGIMEKSQDAGMQTFDGAL
YALVIEGAIDEEEALKHADSVNNLRLRLKLHAEASPGLHAPPGEWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=929155 V0M98_RS13715 WP_338923098.1 2987438..2988547(+) (pilU) [Pseudomonas silesiensis strain G3]
ATGGAAATCGATGCATTGTTGAAGGTCCTGGCCCACCAGGATGGCTCCGATCTTTACCTGTCCACGGGGGCGCCACCCTG
TGCACGGTTCGATGGCGTGCTCAAGCCCCTGGCCGATCAGCCGTTCAAACCCGGGGAAATTGCCGCCATTGCCGCGTCGA
TCATGGACGCCGAACAGCGTCTGGAGTTCGATCGGGAACTGGAGATGAACCTGGCGATCTCGTTGGCGGGTATCGGGCGC
TTTCGGGTCAATATTTTCAAGCAGCGCAACGATGTGTCCATCGTGGTGCGCAACGTCAAGCTCGACATTCCGCGCTTCGA
AGACCTCAAGTTGCCTTCGGTATTGCTCGAAGCGGTGATGCTCAAGCAAGGCCTGATGCTGTTCGTCGGTGCGACAGGCT
CGGGCAAATCAACTTCGCTGGCGGCACTGATCGACTATCGCAACCGCCACAGCAGCGGCCATATCATTACCATCGAAGAC
CCGGTCGAGTACATCCATCGGCACAAGAAGTCGATCATCAACCAGCGTGAGGTCGGCGTCGATACCCGCAGTTTCCATGC
CGCGCTGAAAAATACCCTGCGTCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCGTTTGCCGATACCGGCCATCTGGTGATCTCGACGCTGCATGCGCACAACGCCAATCAGGCACTGGACCGGATC
ATTAATTTTTTCCCCGAAGAACGTCGCGCGCAACTGCTCAATGACCTGGGCAATAATCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTGCCGACTCGCGACGGTCACCGCCGGGCTGCGGTGGAGGTGATGCTGGGCTCGCCGACGATTGGCGACCTGATCC
GGCGCAACGAGTTTGCTGAACTCAAGGGGATCATGGAGAAGTCGCAGGACGCGGGGATGCAGACCTTCGATGGTGCGCTT
TATGCGTTGGTCATCGAAGGGGCGATCGATGAGGAGGAGGCGCTCAAACATGCGGATTCGGTGAACAACTTGCGGTTGCG
CTTGAAGTTGCACGCCGAAGCGTCACCGGGGCTCCATGCCCCTCCCGGCGAGTGGGGACTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.978

99.729

0.558
  pilU Acinetobacter baylyi ADP1

51.667

97.561

0.504
  pilU Vibrio cholerae strain A1552

51.143

94.851

0.485
  pilT Pseudomonas aeruginosa PAK

40.116

93.225

0.374
  pilT Legionella pneumophila strain Lp02

40.719

90.515

0.369
  pilT Legionella pneumophila strain ERS1305867

40.719

90.515

0.369
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

90.244

0.369
  pilT Vibrio cholerae strain A1552

40.841

90.244

0.369
  pilT Pseudomonas stutzeri DSM 10701

39.535

93.225

0.369
  pilT Neisseria meningitidis 8013

38.551

93.496

0.36
  pilT Neisseria gonorrhoeae MS11

38.551

93.496

0.36