Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   VW923_RS08975 Genome accession   NZ_CP142827
Coordinates   1785075..1785350 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain CF4A-3113     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1748096..1785051 1785075..1785350 flank 24


Gene organization within MGE regions


Location: 1748096..1785350
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VW923_RS08670 (VW923_08670) - 1748096..1749103 (-) 1008 WP_138807122.1 class I SAM-dependent methyltransferase -
  VW923_RS08675 (VW923_08675) comGG 1749232..1749585 (-) 354 WP_002360027.1 competence type IV pilus minor pilin ComGG -
  VW923_RS08680 (VW923_08680) comGF 1749585..1750019 (-) 435 WP_002398461.1 competence type IV pilus minor pilin ComGF -
  VW923_RS08685 (VW923_08685) - 1750009..1750380 (-) 372 WP_181444836.1 type II secretion system protein -
  VW923_RS08690 (VW923_08690) - 1750531..1751616 (-) 1086 WP_329443083.1 SH3 domain-containing protein -
  VW923_RS08695 (VW923_08695) - 1751621..1751824 (-) 204 WP_010711247.1 phage holin -
  VW923_RS08700 (VW923_08700) - 1751821..1752042 (-) 222 WP_002397443.1 hypothetical protein -
  VW923_RS08705 (VW923_08705) - 1752070..1752225 (-) 156 WP_002364192.1 XkdX family protein -
  VW923_RS08710 (VW923_08710) - 1752227..1752547 (-) 321 WP_329494946.1 hypothetical protein -
  VW923_RS08715 (VW923_08715) - 1752558..1753046 (-) 489 WP_264547535.1 hypothetical protein -
  VW923_RS08720 (VW923_08720) - 1753046..1753363 (-) 318 WP_010823170.1 hypothetical protein -
  VW923_RS08725 (VW923_08725) - 1753356..1753673 (-) 318 WP_264547534.1 hypothetical protein -
  VW923_RS08730 (VW923_08730) - 1753670..1754575 (-) 906 WP_264547533.1 phage baseplate upper protein -
  VW923_RS08735 (VW923_08735) - 1754594..1757389 (-) 2796 WP_329443077.1 phage tail tip lysozyme -
  VW923_RS08740 (VW923_08740) - 1757386..1758099 (-) 714 WP_174123966.1 phage tail protein -
  VW923_RS08745 (VW923_08745) - 1758096..1760399 (-) 2304 WP_104874255.1 phage tail tape measure protein -
  VW923_RS08750 (VW923_08750) - 1760591..1761046 (-) 456 WP_002395308.1 hypothetical protein -
  VW923_RS08755 (VW923_08755) - 1761046..1761693 (-) 648 WP_010784938.1 major tail protein -
  VW923_RS08760 (VW923_08760) - 1761690..1762052 (-) 363 WP_104858198.1 HK97-gp10 family putative phage morphogenesis protein -
  VW923_RS08765 (VW923_08765) - 1762042..1762380 (-) 339 WP_104858197.1 hypothetical protein -
  VW923_RS08770 (VW923_08770) - 1762370..1762696 (-) 327 WP_104858196.1 phage head closure protein -
  VW923_RS08775 (VW923_08775) - 1762677..1762955 (-) 279 WP_002301326.1 head-tail connector protein -
  VW923_RS08780 (VW923_08780) - 1762957..1764312 (-) 1356 WP_329443055.1 phage major capsid protein -
  VW923_RS08785 (VW923_08785) - 1764324..1764896 (-) 573 WP_002393031.1 HK97 family phage prohead protease -
  VW923_RS08790 (VW923_08790) - 1764883..1766094 (-) 1212 WP_029677624.1 phage portal protein -
  VW923_RS08795 (VW923_08795) - 1766113..1767840 (-) 1728 WP_329443050.1 terminase large subunit -
  VW923_RS08800 (VW923_08800) - 1767837..1768289 (-) 453 WP_002393034.1 P27 family phage terminase small subunit -
  VW923_RS08805 (VW923_08805) - 1768404..1768772 (-) 369 WP_064687383.1 HNH endonuclease -
  VW923_RS08810 (VW923_08810) - 1768772..1769188 (-) 417 WP_002393036.1 hypothetical protein -
  VW923_RS08820 (VW923_08820) - 1769717..1770184 (-) 468 WP_002380819.1 ArpU family phage packaging/lysis transcriptional regulator -
  VW923_RS08825 (VW923_08825) - 1770544..1770750 (-) 207 WP_116491842.1 hypothetical protein -
  VW923_RS08830 (VW923_08830) - 1770764..1771348 (-) 585 WP_185776395.1 DUF3850 domain-containing protein -
  VW923_RS08835 (VW923_08835) - 1771345..1771524 (-) 180 WP_185776396.1 hypothetical protein -
  VW923_RS08840 (VW923_08840) - 1771521..1771682 (-) 162 WP_010774952.1 hypothetical protein -
  VW923_RS08845 (VW923_08845) - 1771679..1771987 (-) 309 WP_002380822.1 hypothetical protein -
  VW923_RS08850 (VW923_08850) - 1771988..1772362 (-) 375 WP_010774953.1 YopX family protein -
  VW923_RS08855 (VW923_08855) - 1772406..1773170 (-) 765 WP_010785682.1 DNA-methyltransferase -
  VW923_RS08860 (VW923_08860) - 1773223..1773429 (-) 207 WP_002417547.1 hypothetical protein -
  VW923_RS08865 (VW923_08865) - 1773439..1773831 (-) 393 WP_185776397.1 hypothetical protein -
  VW923_RS08870 (VW923_08870) - 1773837..1774445 (-) 609 WP_185776398.1 hypothetical protein -
  VW923_RS08875 (VW923_08875) - 1774465..1774935 (-) 471 WP_002380828.1 DUF1064 domain-containing protein -
  VW923_RS08880 (VW923_08880) - 1775084..1775353 (-) 270 WP_185776399.1 hypothetical protein -
  VW923_RS08885 (VW923_08885) - 1775492..1776331 (-) 840 WP_329443017.1 ATP-binding protein -
  VW923_RS08890 (VW923_08890) - 1776343..1777122 (-) 780 WP_329443014.1 DnaD domain protein -
  VW923_RS08895 (VW923_08895) - 1777137..1777952 (-) 816 WP_329443011.1 PD-(D/E)XK nuclease-like domain-containing protein -
  VW923_RS08900 (VW923_08900) - 1777915..1778886 (-) 972 WP_329443008.1 RecT family recombinase -
  VW923_RS08905 (VW923_08905) - 1778961..1779266 (-) 306 WP_002395805.1 hypothetical protein -
  VW923_RS08910 (VW923_08910) - 1779317..1779571 (+) 255 WP_002395804.1 hypothetical protein -
  VW923_RS08915 (VW923_08915) - 1779546..1779764 (-) 219 WP_002370011.1 hypothetical protein -
  VW923_RS08920 (VW923_08920) - 1779868..1780092 (-) 225 WP_010821117.1 hypothetical protein -
  VW923_RS08925 (VW923_08925) - 1780089..1780412 (-) 324 WP_329443002.1 hypothetical protein -
  VW923_RS08930 (VW923_08930) - 1780503..1780919 (-) 417 WP_329442999.1 hypothetical protein -
  VW923_RS08935 (VW923_08935) - 1780920..1781099 (-) 180 WP_016627612.1 hypothetical protein -
  VW923_RS08940 (VW923_08940) - 1781106..1781417 (-) 312 WP_002381719.1 hypothetical protein -
  VW923_RS08945 (VW923_08945) - 1781428..1781604 (-) 177 WP_002364354.1 helix-turn-helix domain-containing protein -
  VW923_RS08950 (VW923_08950) - 1781915..1782238 (+) 324 WP_010826721.1 helix-turn-helix domain-containing protein -
  VW923_RS08955 (VW923_08955) - 1782256..1782600 (+) 345 WP_002395798.1 ImmA/IrrE family metallo-endopeptidase -
  VW923_RS08960 (VW923_08960) - 1782634..1783362 (+) 729 WP_002364358.1 ion transporter -
  VW923_RS08965 (VW923_08965) - 1783459..1784607 (+) 1149 WP_002395797.1 tyrosine-type recombinase/integrase -
  VW923_RS08970 (VW923_08970) comGD 1784635..1785078 (-) 444 WP_002395796.1 competence type IV pilus minor pilin ComGD -
  VW923_RS08975 (VW923_08975) comGC/cglC 1785075..1785350 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=927183 VW923_RS08975 WP_002356991.1 1785075..1785350(-) (comGC/cglC) [Enterococcus faecalis strain CF4A-3113]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=927183 VW923_RS08975 WP_002356991.1 1785075..1785350(-) (comGC/cglC) [Enterococcus faecalis strain CF4A-3113]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396