Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   VW859_RS08805 Genome accession   NZ_CP142809
Coordinates   1782148..1782423 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain KS2H-124     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1742637..1800530 1782148..1782423 within 0


Gene organization within MGE regions


Location: 1742637..1800530
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VW859_RS08530 (VW859_08530) - 1742637..1743644 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  VW859_RS08535 (VW859_08535) comGG 1743773..1744126 (-) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  VW859_RS08540 (VW859_08540) comGF 1744126..1744560 (-) 435 WP_002378441.1 competence type IV pilus minor pilin ComGF -
  VW859_RS08545 (VW859_08545) - 1744550..1744876 (-) 327 WP_002370967.1 type II secretion system protein -
  VW859_RS08550 (VW859_08550) hemH 1745677..1746618 (-) 942 WP_002357056.1 ferrochelatase -
  VW859_RS08560 (VW859_08560) - 1747334..1747606 (-) 273 WP_002378444.1 hypothetical protein -
  VW859_RS08565 (VW859_08565) - 1747678..1747878 (-) 201 WP_002357053.1 cold-shock protein -
  VW859_RS08570 (VW859_08570) - 1748731..1749990 (-) 1260 WP_002378445.1 LysM peptidoglycan-binding domain-containing protein -
  VW859_RS08575 (VW859_08575) - 1750003..1750383 (-) 381 WP_002378446.1 phage holin family protein -
  VW859_RS08580 (VW859_08580) - 1750394..1750516 (-) 123 WP_002368228.1 XkdX family protein -
  VW859_RS08585 (VW859_08585) - 1750518..1750913 (-) 396 WP_002363385.1 hypothetical protein -
  VW859_RS08590 (VW859_08590) - 1750932..1751384 (-) 453 WP_002363384.1 hypothetical protein -
  VW859_RS08595 (VW859_08595) - 1751401..1752141 (-) 741 WP_002363383.1 hypothetical protein -
  VW859_RS08600 (VW859_08600) - 1752147..1753592 (-) 1446 WP_002378447.1 phage tail spike protein -
  VW859_RS08605 (VW859_08605) - 1753592..1754314 (-) 723 WP_002363380.1 hypothetical protein -
  VW859_RS08610 (VW859_08610) - 1754311..1758765 (-) 4455 WP_002378448.1 phage tail tape measure protein -
  VW859_RS08615 (VW859_08615) - 1758752..1759057 (-) 306 WP_002366371.1 hypothetical protein -
  VW859_RS08620 (VW859_08620) - 1759126..1759524 (-) 399 WP_002378449.1 tail assembly chaperone -
  VW859_RS08625 (VW859_08625) - 1759578..1760150 (-) 573 WP_002401805.1 immunoglobulin-like domain-containing protein -
  VW859_RS08630 (VW859_08630) - 1760199..1760381 (-) 183 WP_002378453.1 hypothetical protein -
  VW859_RS08635 (VW859_08635) - 1760384..1760989 (-) 606 WP_002364302.1 phage major tail protein, TP901-1 family -
  VW859_RS08640 (VW859_08640) - 1761008..1761400 (-) 393 WP_002363373.1 DUF3168 domain-containing protein -
  VW859_RS08645 (VW859_08645) - 1761397..1761735 (-) 339 WP_002363372.1 HK97-gp10 family putative phage morphogenesis protein -
  VW859_RS08650 (VW859_08650) - 1761732..1762007 (-) 276 WP_002378454.1 hypothetical protein -
  VW859_RS08655 (VW859_08655) - 1762004..1762336 (-) 333 WP_002363369.1 phage head-tail connector protein -
  VW859_RS08660 (VW859_08660) - 1762407..1763303 (-) 897 WP_002378455.1 hypothetical protein -
  VW859_RS08665 (VW859_08665) - 1763317..1763952 (-) 636 WP_010710204.1 DUF4355 domain-containing protein -
  VW859_RS08670 (VW859_08670) - 1764075..1765001 (-) 927 WP_002378457.1 minor capsid protein -
  VW859_RS08675 (VW859_08675) - 1764994..1766478 (-) 1485 WP_002378458.1 phage portal protein -
  VW859_RS08680 (VW859_08680) - 1766478..1767761 (-) 1284 WP_329430800.1 PBSX family phage terminase large subunit -
  VW859_RS08685 (VW859_08685) - 1767742..1768176 (-) 435 WP_002364295.1 terminase small subunit -
  VW859_RS08690 (VW859_08690) - 1768208..1768438 (-) 231 Protein_1679 hypothetical protein -
  VW859_RS08695 (VW859_08695) - 1768909..1769271 (-) 363 WP_224800088.1 hypothetical protein -
  VW859_RS08700 (VW859_08700) - 1769388..1770296 (-) 909 WP_002378460.1 hypothetical protein -
  VW859_RS08710 (VW859_08710) - 1771011..1771427 (-) 417 WP_002357018.1 ArpU family phage packaging/lysis transcriptional regulator -
  VW859_RS08715 (VW859_08715) - 1772335..1772742 (-) 408 WP_002378462.1 RusA family crossover junction endodeoxyribonuclease -
  VW859_RS08720 (VW859_08720) - 1772739..1773596 (-) 858 WP_002364343.1 helix-turn-helix domain-containing protein -
  VW859_RS08725 (VW859_08725) - 1773596..1773796 (-) 201 WP_002357010.1 hypothetical protein -
  VW859_RS08730 (VW859_08730) - 1773801..1774442 (-) 642 WP_002364344.1 putative HNHc nuclease -
  VW859_RS08735 (VW859_08735) - 1774447..1775181 (-) 735 WP_002378463.1 ERF family protein -
  VW859_RS08740 (VW859_08740) - 1775174..1775491 (-) 318 WP_002357007.1 hypothetical protein -
  VW859_RS08745 (VW859_08745) - 1775711..1776265 (+) 555 WP_002357006.1 hypothetical protein -
  VW859_RS08750 (VW859_08750) - 1776692..1776886 (-) 195 WP_002378464.1 hypothetical protein -
  VW859_RS08755 (VW859_08755) - 1776923..1777132 (-) 210 WP_002378465.1 hypothetical protein -
  VW859_RS08760 (VW859_08760) - 1777187..1777375 (+) 189 WP_329430801.1 YegP family protein -
  VW859_RS08765 (VW859_08765) - 1777401..1778126 (-) 726 WP_002378466.1 Rha family transcriptional regulator -
  VW859_RS08770 (VW859_08770) - 1778165..1778476 (-) 312 WP_002381719.1 hypothetical protein -
  VW859_RS08775 (VW859_08775) - 1778487..1778663 (-) 177 WP_002364354.1 helix-turn-helix domain-containing protein -
  VW859_RS08780 (VW859_08780) - 1778975..1779307 (+) 333 WP_002364355.1 helix-turn-helix domain-containing protein -
  VW859_RS08785 (VW859_08785) - 1779324..1779668 (+) 345 WP_002378467.1 ImmA/IrrE family metallo-endopeptidase -
  VW859_RS08790 (VW859_08790) - 1779704..1780432 (+) 729 WP_002378468.1 ion transporter -
  VW859_RS08795 (VW859_08795) - 1780532..1781680 (+) 1149 WP_002378469.1 tyrosine-type recombinase/integrase -
  VW859_RS08800 (VW859_08800) comGD 1781717..1782151 (-) 435 Protein_1700 competence type IV pilus minor pilin ComGD -
  VW859_RS08805 (VW859_08805) comGC/cglC 1782148..1782423 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  VW859_RS08810 (VW859_08810) comGB 1782423..1783469 (-) 1047 WP_002369171.1 competence type IV pilus assembly protein ComGB -
  VW859_RS08815 (VW859_08815) comGA 1783426..1784394 (-) 969 WP_002364362.1 competence type IV pilus ATPase ComGA -
  VW859_RS08820 (VW859_08820) - 1784635..1785963 (-) 1329 WP_002360022.1 APC family permease -
  VW859_RS08825 (VW859_08825) rlmN 1786253..1787326 (-) 1074 WP_002378471.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  VW859_RS08830 (VW859_08830) - 1787452..1789281 (-) 1830 WP_329430803.1 ABC transporter permease -
  VW859_RS08835 (VW859_08835) - 1789271..1790020 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  VW859_RS08840 (VW859_08840) - 1790138..1790839 (-) 702 WP_002364244.1 GntR family transcriptional regulator -
  VW859_RS08845 (VW859_08845) - 1790970..1793393 (-) 2424 WP_002378474.1 DNA translocase FtsK -
  VW859_RS08850 (VW859_08850) - 1793707..1794918 (-) 1212 WP_002378475.1 NAD(P)/FAD-dependent oxidoreductase -
  VW859_RS08855 (VW859_08855) - 1794947..1795852 (-) 906 WP_002360016.1 prenyltransferase -
  VW859_RS08860 (VW859_08860) - 1795974..1796954 (+) 981 WP_002378476.1 polyprenyl synthetase family protein -
  VW859_RS08865 (VW859_08865) cydC 1797034..1798800 (-) 1767 WP_002364246.1 thiol reductant ABC exporter subunit CydC -
  VW859_RS08870 (VW859_08870) cydD 1798797..1800530 (-) 1734 WP_002378477.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=926892 VW859_RS08805 WP_002356991.1 1782148..1782423(-) (comGC/cglC) [Enterococcus faecalis strain KS2H-124]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=926892 VW859_RS08805 WP_002356991.1 1782148..1782423(-) (comGC/cglC) [Enterococcus faecalis strain KS2H-124]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396