Detailed information    

insolico Bioinformatically predicted

Overview


Name   comGC   Type   Machinery gene
Locus tag   Llc71_RS11960 Genome accession   NZ_AP025520
Coordinates   2333397..2333666 (-) Length   89 a.a.
NCBI ID   WP_042748573.1    Uniprot ID   A0A084A9I1
Organism   Lactococcus cremoris strain 7-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 2331488..2371171 2333397..2333666 within 0


Gene organization within MGE regions


Location: 2331488..2371171
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Llc71_RS11935 (Llc71_23830) - 2331488..2331925 (-) 438 WP_011836041.1 zinc-dependent MarR family transcriptional regulator -
  Llc71_RS11940 (Llc71_23840) comGG 2332006..2332305 (-) 300 WP_011836042.1 competence type IV pilus minor pilin ComGG Machinery gene
  Llc71_RS11945 (Llc71_23860) comGF 2332329..2332775 (-) 447 WP_011836043.1 competence type IV pilus minor pilin ComGF Machinery gene
  Llc71_RS11950 (Llc71_23870) comGE 2332738..2333034 (-) 297 WP_248536738.1 competence type IV pilus minor pilin ComGE Machinery gene
  Llc71_RS11955 (Llc71_23880) comGD 2333006..2333437 (-) 432 WP_052719845.1 competence type IV pilus minor pilin ComGD Machinery gene
  Llc71_RS11960 (Llc71_23890) comGC 2333397..2333666 (-) 270 WP_042748573.1 competence type IV pilus major pilin ComGC Machinery gene
  Llc71_RS11970 (Llc71_23900) - 2334592..2335029 (-) 438 WP_248536995.1 DUF2335 domain-containing protein -
  Llc71_RS11975 (Llc71_23910) - 2335019..2335246 (-) 228 WP_058225013.1 hypothetical protein -
  Llc71_RS11980 (Llc71_23920) - 2335393..2336172 (-) 780 WP_248536739.1 peptidoglycan amidohydrolase family protein -
  Llc71_RS11985 (Llc71_23930) - 2336172..2336459 (-) 288 WP_023164507.1 phage holin -
  Llc71_RS11990 (Llc71_23940) - 2336471..2336821 (-) 351 WP_248536740.1 hypothetical protein -
  Llc71_RS11995 (Llc71_23950) - 2336834..2337070 (-) 237 WP_248536741.1 hypothetical protein -
  Llc71_RS12000 (Llc71_23960) - 2337082..2339643 (-) 2562 WP_248536742.1 hypothetical protein -
  Llc71_RS12005 (Llc71_23970) - 2339644..2341359 (-) 1716 WP_248536743.1 distal tail protein Dit -
  Llc71_RS12010 (Llc71_23980) - 2341359..2345771 (-) 4413 WP_248536744.1 tape measure protein -
  Llc71_RS12015 (Llc71_23990) - 2345771..2346025 (-) 255 WP_101913605.1 hypothetical protein -
  Llc71_RS12020 (Llc71_24000) - 2346130..2346504 (-) 375 WP_046781540.1 tail assembly chaperone -
  Llc71_RS12025 (Llc71_24010) - 2346628..2347161 (-) 534 WP_248536745.1 phage major tail protein, TP901-1 family -
  Llc71_RS12030 (Llc71_24020) - 2347176..2347550 (-) 375 WP_248536746.1 hypothetical protein -
  Llc71_RS12035 (Llc71_24030) - 2347613..2347903 (-) 291 WP_248536747.1 HK97-gp10 family putative phage morphogenesis protein -
  Llc71_RS12040 (Llc71_24040) - 2347912..2348247 (-) 336 WP_248536748.1 hypothetical protein -
  Llc71_RS12045 (Llc71_24050) - 2348244..2348576 (-) 333 WP_248536749.1 phage head-tail connector protein -
  Llc71_RS12050 (Llc71_24060) - 2348563..2348760 (-) 198 WP_014570729.1 HeH/LEM domain-containing protein -
  Llc71_RS12055 (Llc71_24070) - 2348760..2349581 (-) 822 WP_058207139.1 N4-gp56 family major capsid protein -
  Llc71_RS12060 (Llc71_24080) - 2349583..2350245 (-) 663 WP_248536750.1 DUF4355 domain-containing protein -
  Llc71_RS12065 (Llc71_24090) - 2350432..2350704 (+) 273 WP_011834801.1 DUF3892 domain-containing protein -
  Llc71_RS12070 (Llc71_24100) - 2350701..2350859 (-) 159 WP_248536751.1 hypothetical protein -
  Llc71_RS12075 (Llc71_24110) - 2350907..2351950 (-) 1044 WP_248536752.1 minor capsid protein -
  Llc71_RS12080 (Llc71_24120) - 2351954..2353324 (-) 1371 WP_248536753.1 phage portal protein -
  Llc71_RS12085 (Llc71_24130) terL 2353338..2354726 (-) 1389 WP_248536754.1 phage terminase large subunit -
  Llc71_RS12090 (Llc71_24140) - 2354719..2355186 (-) 468 WP_248536755.1 helix-turn-helix domain-containing protein -
  Llc71_RS12095 (Llc71_24150) - 2355612..2356034 (-) 423 WP_014570802.1 RinA family protein -
  Llc71_RS12100 (Llc71_24160) - 2356111..2356419 (-) 309 WP_019292848.1 hypothetical protein -
  Llc71_RS12105 (Llc71_24170) - 2356791..2356940 (+) 150 WP_248536610.1 hypothetical protein -
  Llc71_RS12110 (Llc71_24190) - 2357192..2357518 (-) 327 WP_248536756.1 hypothetical protein -
  Llc71_RS12115 (Llc71_24200) - 2357590..2358009 (-) 420 WP_248536757.1 dUTP diphosphatase -
  Llc71_RS12120 (Llc71_24210) - 2358006..2358611 (-) 606 WP_248536758.1 DUF1642 domain-containing protein -
  Llc71_RS12125 (Llc71_24220) - 2358604..2358810 (-) 207 WP_248536759.1 DUF1125 domain-containing protein -
  Llc71_RS12130 (Llc71_24230) - 2358811..2359077 (-) 267 WP_248536760.1 hypothetical protein -
  Llc71_RS12135 (Llc71_24240) - 2359058..2359252 (-) 195 WP_248536761.1 hypothetical protein -
  Llc71_RS12140 (Llc71_24250) - 2359239..2359565 (-) 327 WP_248536762.1 hypothetical protein -
  Llc71_RS12145 (Llc71_24260) - 2359580..2360104 (-) 525 WP_046782331.1 hypothetical protein -
  Llc71_RS12150 (Llc71_24270) - 2360213..2360452 (-) 240 WP_248536996.1 DUF1031 family protein -
  Llc71_RS12155 (Llc71_24280) - 2360465..2360854 (-) 390 WP_248536763.1 RusA family crossover junction endodeoxyribonuclease -
  Llc71_RS12160 (Llc71_24290) - 2360851..2361576 (-) 726 WP_011676538.1 hypothetical protein -
  Llc71_RS12165 (Llc71_24300) - 2361576..2362364 (-) 789 WP_248536764.1 phage replisome organiser protein -
  Llc71_RS12170 (Llc71_24310) - 2362498..2363001 (-) 504 WP_011834781.1 hypothetical protein -
  Llc71_RS12175 (Llc71_24320) - 2363001..2363720 (-) 720 WP_011834780.1 AAA family ATPase -
  Llc71_RS12180 (Llc71_24330) - 2363729..2364247 (-) 519 WP_248536765.1 host-nuclease inhibitor Gam family protein -
  Llc71_RS12185 (Llc71_24340) - 2364347..2364589 (-) 243 WP_248536766.1 hypothetical protein -
  Llc71_RS12190 (Llc71_24350) - 2364591..2364791 (-) 201 WP_003132896.1 helix-turn-helix transcriptional regulator -
  Llc71_RS12195 (Llc71_24360) - 2364828..2365043 (-) 216 WP_248536767.1 hypothetical protein -
  Llc71_RS12200 (Llc71_24370) - 2365150..2365314 (+) 165 WP_248536768.1 hypothetical protein -
  Llc71_RS12205 (Llc71_24380) - 2365311..2365529 (-) 219 WP_063282493.1 hypothetical protein -
  Llc71_RS12210 (Llc71_24400) - 2365875..2366354 (-) 480 WP_248536769.1 phage antirepressor KilAC domain-containing protein -
  Llc71_RS12215 (Llc71_24410) - 2366388..2366666 (-) 279 WP_248536770.1 Bro-N domain-containing protein -
  Llc71_RS12220 (Llc71_24420) - 2366678..2366917 (-) 240 WP_023189646.1 helix-turn-helix transcriptional regulator -
  Llc71_RS12225 (Llc71_24430) - 2367077..2367901 (+) 825 WP_248536771.1 DUF4393 domain-containing protein -
  Llc71_RS12230 (Llc71_24440) - 2368324..2369211 (+) 888 WP_248536772.1 S24 family peptidase -
  Llc71_RS12235 (Llc71_24450) - 2369269..2369592 (+) 324 WP_248536773.1 hypothetical protein -
  Llc71_RS12240 (Llc71_24460) - 2369714..2371171 (+) 1458 WP_248536774.1 recombinase family protein -

Sequence


Protein


Download         Length: 89 a.a.        Molecular weight: 10110.57 Da        Isoelectric Point: 4.4079

>NTDB_id=92529 Llc71_RS11960 WP_042748573.1 2333397..2333666(-) (comGC) [Lactococcus cremoris strain 7-1]
MLIVLAIISILILLFVPNLIKEKAQVQKTGEAAVVKVVESQAQLYELDHDNDKPNLSELLSAGMITKKQVTAYDDYYDQN
KNEQRNFDD

Nucleotide


Download         Length: 270 bp        

>NTDB_id=92529 Llc71_RS11960 WP_042748573.1 2333397..2333666(-) (comGC) [Lactococcus cremoris strain 7-1]
ATGTTAATTGTGTTGGCAATTATCAGTATTTTAATTTTGCTTTTTGTGCCAAATTTGATAAAAGAAAAAGCACAAGTTCA
AAAAACTGGTGAAGCAGCAGTAGTTAAAGTAGTTGAAAGTCAGGCACAACTTTATGAGTTGGATCATGATAATGATAAGC
CAAACCTATCAGAACTTTTAAGTGCGGGGATGATTACGAAAAAGCAAGTCACGGCCTATGATGATTACTATGACCAGAAT
AAAAATGAACAGCGCAATTTCGATGATTAG

Domains



No domain identified.



Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A084A9I1

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC Lactococcus lactis subsp. cremoris KW2

98.876

100

0.989

  comYC Streptococcus gordonii str. Challis substr. CH1

57.647

95.506

0.551

  comGC/cglC Streptococcus pneumoniae Rx1

52.809

100

0.528

  comGC/cglC Streptococcus pneumoniae D39

52.809

100

0.528

  comGC/cglC Streptococcus pneumoniae R6

52.809

100

0.528

  comGC/cglC Streptococcus pneumoniae TIGR4

52.809

100

0.528

  comYC Streptococcus suis isolate S10

57.534

82.022

0.472

  comGC/cglC Streptococcus mitis SK321

52.632

85.393

0.449

  comYC Streptococcus mutans UA140

52.055

82.022

0.427

  comYC Streptococcus mutans UA159

52.055

82.022

0.427

  comGC/cglC Streptococcus mitis NCTC 12261

52.113

79.775

0.416


Multiple sequence alignment