Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN48_RS07520 Genome accession   NZ_CP141723
Coordinates   1543276..1544220 (+) Length   314 a.a.
NCBI ID   WP_347962685.1    Uniprot ID   -
Organism   Lactococcus formosensis strain PAQ-208     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1538276..1549220
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN48_RS07505 (VNN48_07500) - 1539527..1541131 (-) 1605 WP_407281010.1 GH25 family lysozyme -
  VNN48_RS07510 (VNN48_07505) guaB 1541305..1542786 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN48_RS07515 (VNN48_07510) rpsU 1542911..1543087 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN48_RS07520 (VNN48_07515) comGA 1543276..1544220 (+) 945 WP_347962685.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN48_RS07525 (VNN48_07520) comGB 1544159..1545178 (+) 1020 WP_407281527.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN48_RS07530 (VNN48_07525) comYC 1545197..1545502 (+) 306 WP_017368746.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN48_RS07535 (VNN48_07530) comGD 1545477..1545893 (+) 417 WP_279365405.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN48_RS07540 (VNN48_07535) comGE 1545925..1546158 (+) 234 WP_243416060.1 competence type IV pilus minor pilin ComGE -
  VNN48_RS07545 (VNN48_07540) comGF 1546145..1546579 (+) 435 WP_407281014.1 competence type IV pilus minor pilin ComGF -
  VNN48_RS07550 (VNN48_07545) - 1546815..1547660 (+) 846 WP_017368750.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  VNN48_RS07555 (VNN48_07550) - 1547747..1548616 (-) 870 WP_407281016.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35768.14 Da        Isoelectric Point: 7.0210

>NTDB_id=918935 VNN48_RS07520 WP_347962685.1 1543276..1544220(+) (comGA) [Lactococcus formosensis strain PAQ-208]
MIQVLAKTLLQKAVDQMVHDLYLVALEGKYSLYFRTATERRFERELTLEQGQALIAHMKFLSGMNLGESRRVQLGACTYI
LDKGEQRFRLSTVGDFHGQESLVIRLLHHQQHQLHFWNSEIFNTIIGGRGLYLFSGPVGSGKTSLMYKFAREHFKNQQVI
CIEDPVELVEAEFLQLQVNKVIGNDYDTLIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVIAR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVCEKEKFEEWKPDEWNGQIDQLVADGFISAIAAAREKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918935 VNN48_RS07520 WP_347962685.1 1543276..1544220(+) (comGA) [Lactococcus formosensis strain PAQ-208]
ATGATACAGGTTTTAGCAAAAACCCTTTTACAAAAAGCAGTAGATCAGATGGTGCACGATCTTTATTTAGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTCACCCTTGAGCAAGGGCAAGCAC
TGATTGCACATATGAAATTTTTATCTGGTATGAATCTAGGAGAAAGTAGACGAGTACAGTTGGGGGCTTGCACTTATATC
TTAGATAAAGGTGAGCAGCGCTTCCGTTTATCTACTGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATTCGACTCTT
GCATCATCAACAACACCAACTTCATTTTTGGAATTCAGAGATCTTTAATACTATTATTGGGGGAAGAGGCCTTTACCTTT
TTTCAGGTCCTGTAGGTTCAGGTAAGACCAGTCTTATGTACAAATTTGCAAGAGAGCATTTTAAAAATCAACAAGTTATT
TGTATAGAAGATCCTGTGGAGTTAGTGGAAGCTGAGTTTTTACAACTTCAAGTCAATAAAGTTATAGGAAATGATTACGA
TACCTTGATTAAGCTTTCGCTTCGCCACAGACCTGATTTACTTATTGTTGGTGAAATTAGGGATAGTCAAACGGCAAAAG
CTGTTCTCCGTGCAAGTTTGACTGGGTATACAGTATTCTCTACTGTTCATGCACGCTCAATATCAGGTGTAATTGCACGC
TTAAAAGAACTTGGATTAACTGATTGGGAGCTACAATCCAGTCTTCAGCGGGTGATTTATCAACGATTAATTGCAGGAAA
GGGATTACTTGTTTGTGAAAAGGAAAAGTTTGAAGAATGGAAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTATTGCAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

53.016

100

0.532
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

52.077

99.682

0.519
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

51.923

99.363

0.516
  comGA/cglA/cilD Streptococcus pneumoniae R6

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae D39

51.757

99.682

0.516
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

51.757

99.682

0.516
  comYA Streptococcus mutans UA159

50.633

100

0.51
  comYA Streptococcus mutans UA140

50.633

100

0.51