Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN48_RS07525 Genome accession   NZ_CP141723
Coordinates   1544159..1545178 (+) Length   339 a.a.
NCBI ID   WP_407281527.1    Uniprot ID   -
Organism   Lactococcus formosensis strain PAQ-208     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1539159..1550178
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN48_RS07505 (VNN48_07500) - 1539527..1541131 (-) 1605 WP_407281010.1 GH25 family lysozyme -
  VNN48_RS07510 (VNN48_07505) guaB 1541305..1542786 (+) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN48_RS07515 (VNN48_07510) rpsU 1542911..1543087 (+) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN48_RS07520 (VNN48_07515) comGA 1543276..1544220 (+) 945 WP_347962685.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN48_RS07525 (VNN48_07520) comGB 1544159..1545178 (+) 1020 WP_407281527.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN48_RS07530 (VNN48_07525) comYC 1545197..1545502 (+) 306 WP_017368746.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN48_RS07535 (VNN48_07530) comGD 1545477..1545893 (+) 417 WP_279365405.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN48_RS07540 (VNN48_07535) comGE 1545925..1546158 (+) 234 WP_243416060.1 competence type IV pilus minor pilin ComGE -
  VNN48_RS07545 (VNN48_07540) comGF 1546145..1546579 (+) 435 WP_407281014.1 competence type IV pilus minor pilin ComGF -
  VNN48_RS07550 (VNN48_07545) - 1546815..1547660 (+) 846 WP_017368750.1 metal ABC transporter solute-binding protein, Zn/Mn family -
  VNN48_RS07555 (VNN48_07550) - 1547747..1548616 (-) 870 WP_407281016.1 RluA family pseudouridine synthase -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39422.11 Da        Isoelectric Point: 9.0696

>NTDB_id=918936 VNN48_RS07525 WP_407281527.1 1544159..1545178(+) (comGB) [Lactococcus formosensis strain PAQ-208]
MDLSQLLQLRVKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLNFYEDI
VTQLSLAESHGNVEYTLGLIEEKLIRVLNIRRKLIQVSTYPIVLLAFLIFIMLGLKNYLLPQLEENKGLATYAIQNLPTI
FLGCLFGLLVFFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGVEFLQILDSGQKFEDALRFYPMFTKELALIVEYGELKAKLGQELMVFSEESWLLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918936 VNN48_RS07525 WP_407281527.1 1544159..1545178(+) (comGB) [Lactococcus formosensis strain PAQ-208]
ATGGATTTATCTCAGCTATTGCAGCTGCGCGTGAAAAAATTGAGTTTAGCGAAGCAGATTAAACTTATCCAACTCATGAA
TAATCTTTTCAAAAGTGGTTTCCACCTCTCTGAAATTATTGATTTTCTTGAACGGTCGGGGCTAACAGAAGCATATTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAATGGACAAAGTTTCTCTGGTATTTTAGCGAAGCTAAATTTTTATGAGGATATT
GTGACTCAGCTCTCTTTGGCTGAGTCTCATGGGAATGTTGAGTACACTTTAGGATTGATTGAAGAAAAACTGATACGTGT
TTTGAACATCCGCAGAAAGCTCATCCAAGTCTCCACTTATCCGATTGTTCTTCTGGCATTTCTAATCTTTATCATGCTAG
GATTGAAAAATTATTTACTGCCCCAACTGGAAGAAAACAAAGGATTAGCAACTTATGCCATACAGAATCTTCCGACCATC
TTTTTAGGTTGCCTCTTCGGTTTGCTTGTTTTCTTTTACCTGTGCAGATACTATTGGAAGAAAAAAACTGCTTTAGAAGC
GATGAGGCTGATGGTCAAAATACCTTTTGTTGGCCGTTTTGTCCAGCTTTATTTGACGGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTCGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTGTTGAATTCCTTCAAATATTAGATAGTGGTCAGAAGTTTGAAGATGCCCTTCGATTTTACCCCATGTTTACCAA
GGAGCTGGCATTAATTGTGGAATATGGTGAACTAAAGGCTAAGCTAGGGCAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTTGCTCTTTTTTGAAAGAGTCGAAAGAGCAATGCAACTTATCCAGCCTATTGTTTTCTTGCTGGTGGCTTTGTTGATT
ATATTAATTTATGCTGCAATGCTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

52.663

99.705

0.525
  comGB/cglB Streptococcus mitis NCTC 12261

51.94

98.82

0.513
  comYB Streptococcus gordonii str. Challis substr. CH1

51.497

98.525

0.507
  comGB/cglB Streptococcus pneumoniae R6

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.343

98.82

0.507
  comGB/cglB Streptococcus mitis SK321

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae Rx1

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae D39

51.343

98.82

0.507
  comYB Streptococcus mutans UA140

51.111

92.92

0.475
  comYB Streptococcus mutans UA159

50.794

92.92

0.472