Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   VNN37_RS03355 Genome accession   NZ_CP141707
Coordinates   651838..652782 (-) Length   314 a.a.
NCBI ID   WP_339000850.1    Uniprot ID   -
Organism   Lactococcus garvieae strain M21102102     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 646838..657782
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN37_RS03315 (VNN37_03315) - 647446..648315 (+) 870 WP_339000652.1 RluA family pseudouridine synthase -
  VNN37_RS03320 (VNN37_03320) - 648401..649246 (-) 846 WP_339000654.1 zinc ABC transporter substrate-binding protein -
  VNN37_RS03325 (VNN37_03325) - 649309..649470 (-) 162 WP_264308322.1 hypothetical protein -
  VNN37_RS03330 (VNN37_03330) comGF 649479..649919 (-) 441 WP_339000658.1 competence type IV pilus minor pilin ComGF -
  VNN37_RS03335 (VNN37_03335) comGE 649900..650133 (-) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  VNN37_RS03340 (VNN37_03340) comGD 650165..650581 (-) 417 WP_339000660.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN37_RS03345 (VNN37_03345) comYC 650556..650861 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN37_RS03350 (VNN37_03350) comGB 650880..651899 (-) 1020 WP_339000849.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN37_RS03355 (VNN37_03355) comGA 651838..652782 (-) 945 WP_339000850.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN37_RS03360 (VNN37_03360) rpsU 652971..653147 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN37_RS03365 (VNN37_03365) guaB 653270..654751 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN37_RS03370 (VNN37_03370) - 654920..656374 (+) 1455 WP_264308318.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 314 a.a.        Molecular weight: 35809.00 Da        Isoelectric Point: 6.5037

>NTDB_id=918629 VNN37_RS03355 WP_339000850.1 651838..652782(-) (comGA) [Lactococcus garvieae strain M21102102]
MIQVLAKTLLQKAVDQMVHDLYFVALEGKYSLYFRTATERRFERELDLEQGQALIAHMKFLAGMNLGESRRVQLGACTYV
LDKGEQRLRLSTVGDFHGQESLVIRLLHHQQSQLHFWNSEIFKSFIGGRGLYLFSGPVGSGKTSLMYKFAREHFQNQQVI
CIEDPVELVETEFLQLQVNKVIGNDYDALIKLSLRHRPDLLIVGEIRDSQTAKAVLRASLTGYTVFSTVHARSISGVISR
LKELGLTDWELQSSLQRVIYQRLIAGKGLLVYEKEKFEEWQPDEWNGQIDQLVADGFISAVTAAHEKIEFSEAD

Nucleotide


Download         Length: 945 bp        

>NTDB_id=918629 VNN37_RS03355 WP_339000850.1 651838..652782(-) (comGA) [Lactococcus garvieae strain M21102102]
ATGATACAGGTTTTGGCAAAAACCCTTTTACAAAAAGCAGTAGATCAAATGGTGCATGATCTTTATTTCGTAGCATTAGA
AGGAAAGTATTCTCTCTATTTTCGTACAGCAACAGAAAGGAGGTTTGAAAGAGAACTTGATCTTGAACAAGGGCAAGCAT
TGATTGCTCATATGAAATTTTTAGCTGGTATGAATCTAGGAGAAAGTAGGCGGGTACAGTTGGGGGCTTGCACTTATGTT
TTAGATAAAGGTGAGCAGCGTTTACGTTTATCTACAGTCGGTGATTTTCATGGTCAAGAAAGCTTGGTTATCCGACTTCT
GCATCATCAACAAAGCCAACTTCATTTTTGGAATTCAGAGATTTTTAAATCTTTTATTGGTGGAAGAGGGCTTTACCTGT
TTTCCGGTCCGGTAGGCTCAGGTAAGACCAGCCTCATGTATAAGTTTGCAAGGGAGCATTTTCAAAATCAACAAGTTATT
TGTATAGAAGATCCCGTGGAGTTAGTGGAAACTGAGTTTTTACAACTTCAAGTCAATAAAGTAATAGGGAATGATTACGA
TGCCTTGATTAAGCTCTCTCTTCGCCACAGACCTGATTTACTCATTGTTGGTGAAATTAGGGACAGTCAAACGGCAAAAG
CTGTTCTCCGCGCAAGTTTGACGGGATATACCGTGTTTTCTACTGTTCATGCGCGCTCAATATCGGGTGTAATTTCACGC
CTAAAGGAACTTGGATTAACTGATTGGGAGCTACAATCCAGCCTTCAGCGGGTGATTTATCAGCGCTTAATTGCAGGAAA
GGGGTTGCTTGTTTATGAAAAAGAAAAGTTTGAAGAATGGCAACCAGACGAATGGAATGGCCAGATTGATCAGTTGGTTG
CAGATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-264)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

59.873

100

0.599
  comYA Streptococcus gordonii str. Challis substr. CH1

53.651

100

0.538
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.355

99.682

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae D39

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae R6

53.035

99.682

0.529
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

53.035

99.682

0.529
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

52.396

99.682

0.522
  comYA Streptococcus mutans UA159

51.603

99.363

0.513
  comYA Streptococcus mutans UA140

51.603

99.363

0.513