Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   VNN37_RS03350 Genome accession   NZ_CP141707
Coordinates   650880..651899 (-) Length   339 a.a.
NCBI ID   WP_339000849.1    Uniprot ID   -
Organism   Lactococcus garvieae strain M21102102     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 645880..656899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VNN37_RS03315 (VNN37_03315) - 647446..648315 (+) 870 WP_339000652.1 RluA family pseudouridine synthase -
  VNN37_RS03320 (VNN37_03320) - 648401..649246 (-) 846 WP_339000654.1 zinc ABC transporter substrate-binding protein -
  VNN37_RS03325 (VNN37_03325) - 649309..649470 (-) 162 WP_264308322.1 hypothetical protein -
  VNN37_RS03330 (VNN37_03330) comGF 649479..649919 (-) 441 WP_339000658.1 competence type IV pilus minor pilin ComGF -
  VNN37_RS03335 (VNN37_03335) comGE 649900..650133 (-) 234 WP_229031266.1 competence type IV pilus minor pilin ComGE -
  VNN37_RS03340 (VNN37_03340) comGD 650165..650581 (-) 417 WP_339000660.1 competence type IV pilus minor pilin ComGD Machinery gene
  VNN37_RS03345 (VNN37_03345) comYC 650556..650861 (-) 306 WP_016171026.1 competence type IV pilus major pilin ComGC Machinery gene
  VNN37_RS03350 (VNN37_03350) comGB 650880..651899 (-) 1020 WP_339000849.1 competence type IV pilus assembly protein ComGB Machinery gene
  VNN37_RS03355 (VNN37_03355) comGA 651838..652782 (-) 945 WP_339000850.1 competence type IV pilus ATPase ComGA Machinery gene
  VNN37_RS03360 (VNN37_03360) rpsU 652971..653147 (-) 177 WP_003134539.1 30S ribosomal protein S21 -
  VNN37_RS03365 (VNN37_03365) guaB 653270..654751 (-) 1482 WP_003133714.1 IMP dehydrogenase -
  VNN37_RS03370 (VNN37_03370) - 654920..656374 (+) 1455 WP_264308318.1 GH25 family lysozyme -

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 39495.24 Da        Isoelectric Point: 9.6496

>NTDB_id=918628 VNN37_RS03350 WP_339000849.1 650880..651899(-) (comGB) [Lactococcus garvieae strain M21102102]
MDLSQLLQLRTKKLSLAKQIKLIQLMNNLFKSGFHLSEIIDFLERSGLTEAYFISKMREGLLNGQSFSGILAKLKFNEDI
VTQLSLAESHGNVEYTLGLIEEKLRRVLNIRKKLIQVATYPLVLLVFLIFIMLGLKNYLLPQLDENRGLATYVIQNLPTL
FLGCLFSLLVVFYLCRYYWKKKTALEAMRLMVKIPFVGRFVQLYLTAYFSREWGNLIAQAVDLRQICFIMQEQKSRIFKE
FGFELLQILDRGQKFEDALRFYPIFTKELALIVEYGELKSKLGKELMVFSEESWSLFFERVERAMQLIQPIVFLLVALLI
ILIYAAMLLPIYSNMGNLI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=918628 VNN37_RS03350 WP_339000849.1 650880..651899(-) (comGB) [Lactococcus garvieae strain M21102102]
ATGGATTTATCTCAGCTGTTACAGCTGCGCACGAAAAAATTGAGTTTAGCGAAGCAGATTAAGCTTATTCAACTTATGAA
TAATCTTTTCAAAAGTGGCTTTCACCTTTCTGAAATTATTGATTTTCTTGAACGGTCGGGTCTCACGGAAGCATACTTTA
TTTCCAAAATGCGTGAAGGTTTACTCAACGGGCAAAGTTTCTCTGGTATTTTAGCGAAGCTCAAATTTAATGAGGATATT
GTGACTCAACTCTCTTTGGCCGAGTCTCATGGGAATGTTGAGTATACATTAGGGCTGATTGAAGAAAAACTGAGACGCGT
TTTGAACATTCGCAAAAAGCTCATCCAAGTTGCCACTTACCCGCTCGTTCTTCTGGTATTTCTAATCTTTATCATGCTAG
GGTTAAAAAATTATTTACTGCCCCAATTGGACGAAAACAGAGGATTAGCAACTTATGTTATACAGAATCTTCCGACCCTC
TTTTTAGGGTGCCTCTTCAGTTTGCTTGTTGTCTTTTATCTGTGCAGATACTATTGGAAGAAGAAAACTGCTTTAGAAGC
GATGAGGCTAATGGTCAAAATCCCATTTGTTGGCCGTTTTGTCCAGCTTTATTTGACAGCTTATTTTTCAAGAGAATGGG
GAAACTTAATTGCCCAAGCAGTTGATTTACGTCAGATTTGTTTTATTATGCAAGAACAAAAAAGTCGAATCTTCAAAGAA
TTTGGTTTTGAGCTCCTTCAAATATTAGATAGGGGTCAGAAGTTTGAAGACGCCCTTCGATTTTATCCCATATTTACCAA
GGAGTTGGCATTAATTGTGGAATATGGTGAACTAAAGTCTAAGCTAGGGAAAGAGTTAATGGTTTTTTCTGAAGAAAGCT
GGTCGCTCTTTTTTGAAAGAGTTGAAAGAGCAATGCAACTTATCCAGCCTATCGTTTTCTTGCTCGTGGCGCTGTTGATT
ATTTTAATTTATGCTGCAATGTTACTTCCTATATATAGCAATATGGGAAACTTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

54.438

99.705

0.543
  comGB/cglB Streptococcus mitis NCTC 12261

53.134

98.82

0.525
  comGB/cglB Streptococcus pneumoniae D39

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae R6

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae TIGR4

52.836

98.82

0.522
  comGB/cglB Streptococcus mitis SK321

52.836

98.82

0.522
  comGB/cglB Streptococcus pneumoniae Rx1

52.836

98.82

0.522
  comYB Streptococcus gordonii str. Challis substr. CH1

52.395

98.525

0.516
  comYB Streptococcus mutans UA140

51.746

92.92

0.481
  comYB Streptococcus mutans UA159

51.429

92.92

0.478