Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PsasTeo6_RS03485 Genome accession   NZ_CP139776
Coordinates   749792..750202 (+) Length   136 a.a.
NCBI ID   WP_047934243.1    Uniprot ID   -
Organism   Pseudomonas asiatica strain Teo6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 744792..755202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PsasTeo6_RS03455 (PsasTeo6_05488) - 745318..745749 (-) 432 WP_015268860.1 hypothetical protein -
  PsasTeo6_RS03460 (PsasTeo6_05496) - 745836..746543 (+) 708 WP_322265223.1 energy-coupling factor ABC transporter permease -
  PsasTeo6_RS03465 (PsasTeo6_05504) yacG 746679..746879 (-) 201 WP_003260624.1 DNA gyrase inhibitor YacG -
  PsasTeo6_RS03470 (PsasTeo6_05512) coaE 746876..747499 (-) 624 WP_013970769.1 dephospho-CoA kinase -
  PsasTeo6_RS03475 (PsasTeo6_05520) pilD 747499..748365 (-) 867 WP_038408633.1 A24 family peptidase Machinery gene
  PsasTeo6_RS03480 (PsasTeo6_05528) - 748368..749576 (-) 1209 WP_247846870.1 type II secretion system F family protein -
  PsasTeo6_RS03485 (PsasTeo6_05536) pilA 749792..750202 (+) 411 WP_047934243.1 pilin Machinery gene
  PsasTeo6_RS03495 (PsasTeo6_05552) - 750514..751842 (+) 1329 WP_322265224.1 phage integrase central domain-containing protein -
  PsasTeo6_RS03500 - 752736..752909 (+) 174 Protein_667 integrase -
  PsasTeo6_RS03505 (PsasTeo6_05568) - 753236..753610 (-) 375 WP_085592532.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 13783.04 Da        Isoelectric Point: 7.0812

>NTDB_id=910902 PsasTeo6_RS03485 WP_047934243.1 749792..750202(+) (pilA) [Pseudomonas asiatica strain Teo6]
MKGQRGITLIELMIVVAIIGILATIALPMYTNHQSRSKAAAGLLEISALKTAMDLRLNDGKDVPDVAALGGQPATAHCAI
TASGKAADGTGSIVCTLVDAPANALGKALTLTRSASGWACTTDIEEDLAPKGCKAP

Nucleotide


Download         Length: 411 bp        

>NTDB_id=910902 PsasTeo6_RS03485 WP_047934243.1 749792..750202(+) (pilA) [Pseudomonas asiatica strain Teo6]
ATGAAAGGGCAACGCGGTATCACCCTGATCGAACTGATGATCGTCGTCGCGATCATCGGCATTCTGGCGACCATCGCCTT
ACCGATGTACACCAACCACCAGTCACGCTCCAAGGCCGCGGCCGGGTTGCTGGAGATCAGCGCGCTGAAGACGGCGATGG
ACCTGCGGTTGAATGACGGGAAGGATGTGCCGGATGTGGCCGCGCTGGGTGGCCAGCCGGCGACGGCGCACTGTGCGATC
ACGGCTAGCGGCAAGGCGGCGGATGGCACTGGGAGCATCGTTTGCACGCTGGTGGATGCGCCGGCCAATGCGCTGGGCAA
GGCACTGACCCTGACGCGTTCGGCTAGCGGGTGGGCGTGTACGACCGACATCGAGGAAGACCTGGCGCCGAAGGGGTGCA
AGGCGCCTTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

43.137

100

0.485
  pilA Acinetobacter baumannii strain A118

44.604

100

0.456
  pilA/pilA1 Eikenella corrodens VA1

37.58

100

0.434
  comP Acinetobacter baylyi ADP1

39.456

100

0.426
  pilA/pilAII Pseudomonas stutzeri DSM 10701

42.963

99.265

0.426
  pilA/pilAI Pseudomonas stutzeri DSM 10701

42.647

100

0.426
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

37.063

100

0.39
  pilA Vibrio cholerae strain A1552

37.063

100

0.39
  pilA Vibrio cholerae C6706

37.063

100

0.39
  pilA Vibrio parahaemolyticus RIMD 2210633

38.462

95.588

0.368