Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   SHT67_RS00085 Genome accession   NZ_CP138653
Coordinates   8832..9107 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain ES-109     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 179..8808 8832..9107 flank 24


Gene organization within MGE regions


Location: 179..9107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHT67_RS00010 (SHT67_00010) - 179..976 (-) 798 WP_085443645.1 helix-turn-helix domain-containing protein -
  SHT67_RS00015 (SHT67_00015) - 980..1621 (-) 642 WP_085443647.1 putative HNHc nuclease -
  SHT67_RS00020 (SHT67_00020) - 1618..2556 (-) 939 WP_085443648.1 DUF1351 domain-containing protein -
  SHT67_RS00025 (SHT67_00025) - 2556..3299 (-) 744 WP_010716887.1 ERF family protein -
  SHT67_RS00030 (SHT67_00030) - 3460..3687 (-) 228 WP_085443650.1 hypothetical protein -
  SHT67_RS00035 (SHT67_00035) - 3698..3883 (-) 186 WP_085443651.1 hypothetical protein -
  SHT67_RS00040 (SHT67_00040) - 3894..4604 (-) 711 WP_085443652.1 Rha family transcriptional regulator -
  SHT67_RS00045 (SHT67_00045) - 4681..5130 (+) 450 WP_033781497.1 hypothetical protein -
  SHT67_RS00050 (SHT67_00050) - 5277..5417 (-) 141 WP_179141131.1 hypothetical protein -
  SHT67_RS00055 (SHT67_00055) - 5617..5928 (-) 312 WP_085443653.1 hypothetical protein -
  SHT67_RS00060 (SHT67_00060) - 5939..6115 (-) 177 WP_002364354.1 helix-turn-helix transcriptional regulator -
  SHT67_RS00065 (SHT67_00065) - 6427..6750 (+) 324 WP_010826721.1 helix-turn-helix domain-containing protein -
  SHT67_RS00070 (SHT67_00070) - 6768..7112 (+) 345 WP_016627610.1 ImmA/IrrE family metallo-endopeptidase -
  SHT67_RS00075 (SHT67_00075) - 7216..8364 (+) 1149 WP_085443655.1 site-specific integrase -
  SHT67_RS00080 (SHT67_00080) comGD 8398..8835 (-) 438 WP_016627608.1 competence type IV pilus minor pilin ComGD -
  SHT67_RS00085 (SHT67_00085) comGC/cglC 8832..9107 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=904040 SHT67_RS00085 WP_002356991.1 8832..9107(-) (comGC/cglC) [Enterococcus faecalis strain ES-109]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=904040 SHT67_RS00085 WP_002356991.1 8832..9107(-) (comGC/cglC) [Enterococcus faecalis strain ES-109]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396