Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   M2497_RS03000 Genome accession   NZ_AP025169
Coordinates   670518..671843 (+) Length   441 a.a.
NCBI ID   WP_248488166.1    Uniprot ID   -
Organism   Pseudomonas sp. CYM-20-01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 665518..676843
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2497_RS02980 (cym2001_05880) - 665599..668061 (-) 2463 WP_071497104.1 penicillin-binding protein 1A -
  M2497_RS02985 (cym2001_05890) pilM 668281..668934 (+) 654 WP_248485777.1 type IV pilus assembly protein PilM -
  M2497_RS02990 (cym2001_05900) - 668931..669485 (+) 555 WP_248485778.1 PilN domain-containing protein -
  M2497_RS02995 (cym2001_05920) - 669482..670447 (+) 966 WP_248485779.1 pilus assembly protein PilP -
  M2497_RS03000 (cym2001_05930) pilQ 670518..671843 (+) 1326 WP_248488166.1 type IV pilus secretin PilQ family protein Machinery gene
  M2497_RS03005 (cym2001_05940) aroK 671848..672366 (+) 519 WP_003187914.1 shikimate kinase AroK -
  M2497_RS03010 (cym2001_05950) aroB 672492..673592 (+) 1101 WP_021492321.1 3-dehydroquinate synthase -
  M2497_RS03015 (cym2001_05960) - 673603..675162 (+) 1560 WP_248485780.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47736.65 Da        Isoelectric Point: 4.7649

>NTDB_id=90218 M2497_RS03000 WP_248488166.1 670518..671843(+) (pilQ) [Pseudomonas sp. CYM-20-01]
MAIAVPNRLDLIQLPPPGNATSGAYTGDKLNLNFQSIELRTALQQIADVAGLNLVASDDVQGSITLRLKDVPWDQALDLV
LQAKGLDKRVKAGVLLVAPAEELAARELLTLESRKQMAELAPLRRELLQVNYAKAADLAKLFQSVTGLEGVTDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDVPVRQVMIEARIVEANVDYDKSLGARWGGRLTRGNWGAGGIPKPLADGAEPAE
NPPSSPFVDLGSLTGSSGLGIAFITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESSSSGATSV
SFKEASLSLEVTPQITPDDRVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSTTQSKVVDKVPF
LGDVPYLGRLFRRDVLAEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=90218 M2497_RS03000 WP_248488166.1 670518..671843(+) (pilQ) [Pseudomonas sp. CYM-20-01]
ATGGCTATTGCTGTGCCCAATCGTCTAGACCTGATCCAGTTGCCACCGCCTGGCAATGCCACGTCGGGCGCCTACACCGG
CGATAAGCTGAACCTTAACTTCCAGAGCATCGAGTTGCGCACCGCGCTGCAACAGATTGCCGATGTAGCGGGCCTCAATC
TGGTAGCCAGCGACGATGTGCAGGGCTCGATTACCCTGCGCCTCAAGGATGTGCCCTGGGATCAGGCGTTGGACCTGGTG
CTGCAAGCCAAGGGCCTGGATAAGCGGGTAAAGGCCGGTGTATTGCTGGTCGCGCCGGCCGAGGAGTTGGCGGCGCGTGA
GCTGCTGACCCTGGAATCGCGCAAGCAAATGGCTGAACTGGCGCCGTTGCGTCGGGAGCTGTTGCAGGTCAATTACGCCA
AGGCGGCGGACCTGGCCAAGTTGTTTCAGTCAGTCACGGGCCTTGAGGGCGTCACCGACGAGCGTGGCTCGGTGGCGGTG
GATGATCGCACCAACAACATCATCGCCTATCAGACTGGCGAGCGGCTGGAGGAGCTGCGCAGGATCGTGGCGCAGTTGGA
TGTGCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGGCCAATGTCGATTACGACAAAAGCCTGGGCGCTCGCT
GGGGTGGGCGGCTCACTCGCGGCAACTGGGGCGCCGGCGGTATTCCGAAGCCTCTGGCGGATGGCGCGGAGCCTGCGGAA
AACCCACCCAGTTCACCTTTTGTGGACTTGGGGTCACTCACCGGCTCATCGGGCCTGGGCATCGCCTTTATCACCGACAA
CCTGCTGCTGGACCTGGAGCTCACCGCCATGGAGAAAACCGGCAACGGCGAAATCGTCTCGCAACCCAAGGTGGTCACTT
CCGACAAGGAAACCGCACGCATCCTCAAGGGCACCGAGATTCCCTACCAGGAATCCAGCTCCAGCGGCGCGACCTCGGTG
TCGTTCAAGGAAGCCTCGCTGTCGCTGGAGGTCACCCCGCAAATCACCCCTGACGACCGCGTGATCATGGAGGTGAAAGT
CACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGATGTGCCGCCGATCAAGAAAAACGAGGTTAACGCCAAGGTGC
TGGTCAAGGATGGCGAGACCATCGTCATTGGCGGGGTTTTCTCCACTACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTGCTGGCGGAGAAAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(53-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.396

99.093

0.707
  pilQ Acinetobacter baumannii D1279779

40.162

100

0.449
  pilQ Acinetobacter baumannii strain A118

40.162

100

0.449
  comQ Acinetobacter baylyi ADP1

42.094

100

0.429
  pilQ Vibrio campbellii strain DS40M4

43.972

95.918

0.422
  pilQ Legionella pneumophila strain Lp02

43.81

95.238

0.417
  pilQ Legionella pneumophila strain ERS1305867

43.705

95.465

0.417
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.301

94.785

0.41
  pilQ Vibrio cholerae strain A1552

43.301

94.785

0.41
  pilQ Neisseria meningitidis M1080

36.245

100

0.376
  pilQ Neisseria meningitidis MC58

36.344

100

0.374
  pilQ Neisseria gonorrhoeae strain FA1090

35.022

100

0.361

Multiple sequence alignment