NTDB

Overview

Name	pilA	Type	Machinery gene
Locus tag	R6I07_RS23450	Genome accession	NZ_CP137694
Coordinates	5068494..5068943 (-)	Length	149 a.a.
NCBI ID	WP_003112842.1	Uniprot ID	P04739
Organism	Pseudomonas aeruginosa strain PAO1F
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5063494..5073943

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
R6I07_RS23430 (R6I07_23430)	ampD	5064186..5064752 (-)	567	WP_003112844.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
R6I07_RS23435 (R6I07_23435)	-	5064903..5067158 (-)	2256	WP_003112843.1	DUF1631 domain-containing protein	-
R6I07_RS23440 (R6I07_23440)	nadC	5067444..5068292 (+)	849	WP_003104915.1	carboxylating nicotinate-nucleotide diphosphorylase	-
R6I07_RS23450 (R6I07_23450)	pilA	5068494..5068943 (-)	450	WP_003112842.1	type 4a pilus biogenesis protein PilA	Machinery gene
R6I07_RS23455 (R6I07_23455)	pilB	5069175..5070875 (+)	1701	WP_003112841.1	type IV-A pilus assembly ATPase PilB	Machinery gene
R6I07_RS23460 (R6I07_23460)	pilC	5070879..5072099 (+)	1221	WP_003112840.1	type 4a pilus biogenesis protein PilC	Machinery gene
R6I07_RS23465 (R6I07_23465)	pilD	5072103..5072975 (+)	873	WP_003112839.1	type IV prepilin peptidase/methyltransferase PilD	Machinery gene
R6I07_RS23470 (R6I07_23470)	coaE	5072972..5073583 (+)	612	WP_003112838.1	dephospho-CoA kinase	-
R6I07_RS23475 (R6I07_23475)	yacG	5073580..5073780 (+)	201	WP_003094656.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

Download Length: 149 a.a. Molecular weight: 15511.78 Da Isoelectric Point: 6.2905

                                >NTDB_id=899818 R6I07_RS23450 WP_003112842.1 5068494..5068943(-) (pilA) [Pseudomonas aeruginosa strain PAO1F]

MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV

GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide

Download Length: 450 bp

                                >NTDB_id=899818 R6I07_RS23450 WP_003112842.1 5068494..5068943(-) (pilA) [Pseudomonas aeruginosa strain PAO1F]

ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT

TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG

AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC

GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC

CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT

GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
PDB	1PAN
PDB	1PAO
PDB	8TUM
PDB	8TUW
PDB	9EWX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilA	Pseudomonas aeruginosa PAK	67.785	100	0.678
pilA	Acinetobacter baumannii strain A118	48	100	0.483
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	48.322	100	0.483
pilA	Vibrio cholerae strain A1552	48.322	100	0.483
pilA	Vibrio cholerae C6706	48.322	100	0.483
pilA/pilAI	Pseudomonas stutzeri DSM 10701	45.638	100	0.456
pilA	Vibrio parahaemolyticus RIMD 2210633	44.898	98.658	0.443
comP	Acinetobacter baylyi ADP1	41.83	100	0.43
pilA/pilAII	Pseudomonas stutzeri DSM 10701	44.056	95.973	0.423
pilA2	Legionella pneumophila str. Paris	38.776	98.658	0.383
pilA2	Legionella pneumophila strain ERS1305867	38.095	98.658	0.376

Detailed information NTG0899818