Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   R4540_RS12085 Genome accession   NZ_CP137135
Coordinates   2652148..2653263 (-) Length   371 a.a.
NCBI ID   WP_000654772.1    Uniprot ID   A0A0H5U567
Organism   Vibrio cholerae V52     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2647148..2658263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4540_RS12055 (R4540_12050) hemF 2647732..2648649 (-) 918 WP_000443976.1 oxygen-dependent coproporphyrinogen oxidase -
  R4540_RS12060 (R4540_12055) - 2648668..2649225 (-) 558 WP_001911770.1 L-threonylcarbamoyladenylate synthase -
  R4540_RS12065 (R4540_12060) purE 2649392..2649877 (+) 486 WP_000215768.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  R4540_RS12070 (R4540_12065) - 2649881..2651014 (+) 1134 WP_001265400.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  R4540_RS12075 (R4540_12070) - 2651087..2651653 (-) 567 WP_000087525.1 type I DNA topoisomerase -
  R4540_RS12080 (R4540_12075) - 2651669..2652145 (-) 477 WP_000979769.1 DUF494 family protein -
  R4540_RS12085 (R4540_12080) dprA 2652148..2653263 (-) 1116 WP_000654772.1 DNA-processing protein DprA Machinery gene
  R4540_RS12090 (R4540_12085) - 2653263..2654390 (-) 1128 WP_000113383.1 LysM peptidoglycan-binding domain-containing protein -
  R4540_RS12095 (R4540_12090) def 2654530..2655039 (+) 510 WP_000115010.1 peptide deformylase -
  R4540_RS12100 (R4540_12095) fmt 2655057..2656004 (+) 948 WP_000083540.1 methionyl-tRNA formyltransferase -
  R4540_RS12105 (R4540_12100) rsmB 2656060..2657340 (+) 1281 WP_001102807.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40035.01 Da        Isoelectric Point: 7.1834

>NTDB_id=896449 R4540_RS12085 WP_000654772.1 2652148..2653263(-) (dprA) [Vibrio cholerae V52]
MKDQDLAAWLALCFTPKLGSKTISHLLATRLPAQLQSFTPKQWLACGLKPEQLVFLTTQAAKQAEQCLQWRSAANNRYIV
TPHCPLYPRLLKEINSSPPVLFIEGIWEAVHDPAVAIVGSRNASVDGRQIARQFATELAQSGLVVTSGLALGIDGYAHDG
ALQAQGQTVAVLGSGLAQVYPKQHQGLAERIIAQGALVSEFAPHTPPKADHFPRRNRIISGLSLGVVVVEAAEKSGSLIT
ARYAAEQGREVFVVPGSIFNAASQGSNQLIRQGACLVQSVQQIHQELKNALTWSLSEQVPYQATLFSAVQSDEELPFPEL
LANVGIEATPIDILASRTQIPVQDIMMQLLELELLGHVVAVPGGYIRKGRG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=896449 R4540_RS12085 WP_000654772.1 2652148..2653263(-) (dprA) [Vibrio cholerae V52]
ATGAAAGATCAGGATTTAGCGGCATGGTTGGCGCTCTGTTTTACTCCTAAACTAGGCAGCAAAACCATTTCTCACCTGCT
TGCGACCCGTTTGCCAGCCCAGTTGCAAAGTTTTACGCCTAAGCAATGGTTGGCCTGCGGGCTTAAGCCCGAACAACTGG
TGTTTTTAACCACTCAAGCGGCTAAACAAGCCGAGCAGTGTTTGCAATGGCGATCAGCAGCCAATAACCGCTATATCGTC
ACTCCTCATTGCCCGCTTTACCCTCGTTTATTGAAAGAGATTAACTCATCGCCTCCCGTCCTGTTTATTGAAGGAATATG
GGAAGCGGTGCATGACCCTGCGGTGGCTATCGTCGGTAGCCGCAATGCCAGTGTTGATGGGCGGCAGATCGCTCGCCAGT
TTGCCACTGAGCTCGCGCAGTCAGGTTTAGTGGTCACCAGTGGTTTAGCGCTTGGTATTGATGGCTATGCGCACGATGGC
GCTTTGCAAGCACAAGGGCAAACCGTAGCAGTATTAGGTTCAGGGCTGGCGCAGGTTTACCCCAAACAGCATCAAGGGTT
AGCGGAGCGAATCATCGCCCAAGGGGCCTTGGTTTCTGAGTTTGCCCCTCACACACCGCCTAAAGCCGATCACTTTCCGC
GCCGTAACCGAATTATCAGCGGCTTATCGCTCGGTGTTGTGGTGGTAGAAGCTGCGGAGAAAAGCGGCTCACTCATCACT
GCACGCTACGCGGCTGAGCAAGGGCGTGAGGTCTTTGTGGTTCCCGGATCAATTTTTAATGCCGCCAGCCAAGGTAGCAA
CCAATTGATTCGCCAAGGCGCTTGTTTGGTGCAAAGTGTGCAACAAATTCATCAAGAGCTCAAAAATGCGCTGACTTGGT
CACTCTCTGAACAAGTTCCTTATCAAGCAACACTTTTTTCTGCTGTACAGAGCGATGAAGAATTGCCATTTCCCGAGCTG
TTAGCTAACGTAGGAATAGAAGCTACACCTATTGATATTCTCGCAAGCCGGACCCAGATACCGGTGCAAGATATCATGAT
GCAGCTCTTGGAGCTTGAGCTCCTTGGGCATGTGGTTGCAGTACCTGGTGGCTATATTAGAAAGGGGAGAGGCTAG

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(319-364)

DNA recombination-mediator protein A (DNA_processg_A)

(77-278)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H5U567

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio cholerae strain A1552

100

100

1
  dprA Vibrio campbellii strain DS40M4

58.108

99.73

0.58
  dprA Glaesserella parasuis strain SC1401

43.883

100

0.445
  dprA Legionella pneumophila strain ERS1305867

43.048

100

0.434
  dprA Neisseria meningitidis MC58

37.845

100

0.407
  dprA Neisseria meningitidis strain C311

37.845

100

0.407
  dprA Haemophilus influenzae Rd KW20

44.444

89.757

0.399
  dprA Neisseria gonorrhoeae strain FA1090

36.658

100

0.396
  dprA Acinetobacter baumannii D1279779

43.505

89.218

0.388
  dprA Neisseria gonorrhoeae MS11

35.84

100

0.385
  dprA Acinetobacter baumannii strain A118

43.202

89.218

0.385
  dprA Acinetobacter baylyi ADP1

41.742

89.757

0.375