Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R4702_RS06465 Genome accession   NZ_CP137106
Coordinates   1277563..1278504 (+) Length   313 a.a.
NCBI ID   WP_024478452.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 16P4028     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1272563..1283504
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4702_RS06445 - 1272629..1274446 (+) 1818 WP_001220844.1 acyltransferase family protein -
  R4702_RS06450 nagA 1274599..1275750 (+) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R4702_RS06455 - 1275913..1276971 (+) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  R4702_RS06460 - 1277122..1277487 (+) 366 WP_000286415.1 DUF1033 family protein -
  R4702_RS06465 comGA/cglA/cilD 1277563..1278504 (+) 942 WP_024478452.1 competence type IV pilus ATPase ComGA Machinery gene
  R4702_RS06470 comGB/cglB 1278452..1279468 (+) 1017 WP_075218762.1 competence type IV pilus assembly protein ComGB Machinery gene
  R4702_RS06475 comGC/cglC 1279470..1279796 (+) 327 WP_000738632.1 comG operon protein ComGC Machinery gene
  R4702_RS06480 comGD/cglD 1279789..1280193 (+) 405 WP_000588020.1 competence type IV pilus minor pilin ComGD Machinery gene
  R4702_RS06485 comGE/cglE 1280156..1280458 (+) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R4702_RS06490 comGF/cglF 1280421..1280882 (+) 462 WP_000250531.1 competence type IV pilus minor pilin ComGF Machinery gene
  R4702_RS06495 comGG/cglG 1280860..1281273 (+) 414 WP_000265631.1 competence type IV pilus minor pilin ComGG Machinery gene
  R4702_RS06500 - 1281410..1282004 (+) 595 Protein_1274 class I SAM-dependent methyltransferase -
  R4702_RS06505 comYH 1282065..1283018 (+) 954 WP_000345100.1 class I SAM-dependent methyltransferase Machinery gene

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35550.45 Da        Isoelectric Point: 6.0083

>NTDB_id=895563 R4702_RS06465 WP_024478452.1 1277563..1278504(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16P4028]
MVQEIAQEIIRSARKKGAQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEEFAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=895563 R4702_RS06465 WP_024478452.1 1277563..1278504(+) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 16P4028]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCGGCTCGGAAAAAAGGGGCGCAAGACATTTATTTTGTCCCTAAGTT
AGATGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTTGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.166

100

0.962
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.544

86.901

0.361