Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   R4714_RS05615 Genome accession   NZ_CP137099
Coordinates   1029982..1031280 (-) Length   432 a.a.
NCBI ID   WP_000867605.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 20824-4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024982..1036280
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R4714_RS05595 rplI 1026137..1026589 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  R4714_RS05600 - 1026586..1028559 (-) 1974 WP_000715109.1 DHH family phosphoesterase -
  R4714_RS05605 hpf 1028695..1029243 (-) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  R4714_RS05610 comFC/cflB 1029323..1029985 (-) 663 WP_000649974.1 ComF family protein Machinery gene
  R4714_RS05615 comFA/cflA 1029982..1031280 (-) 1299 WP_000867605.1 DEAD/DEAH box helicase Machinery gene
  R4714_RS05620 - 1031336..1031971 (+) 636 WP_000395739.1 YigZ family protein -
  R4714_RS05625 cysK 1032256..1033176 (+) 921 WP_000029866.1 cysteine synthase A -
  R4714_RS05630 - 1033251..1033789 (-) 539 Protein_1064 transposase -
  R4714_RS05635 - 1033762..1034070 (+) 309 Protein_1065 transposase -
  R4714_RS05640 tnpB 1034206..1034547 (-) 342 WP_050208954.1 IS66 family insertion sequence element accessory protein TnpB -
  R4714_RS05645 tsf 1034793..1035833 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49620.59 Da        Isoelectric Point: 8.4382

>NTDB_id=895034 R4714_RS05615 WP_000867605.1 1029982..1031280(-) (comFA/cflA) [Streptococcus pneumoniae strain 20824-4]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=895034 R4714_RS05615 WP_000867605.1 1029982..1031280(-) (comFA/cflA) [Streptococcus pneumoniae strain 20824-4]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCAATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae D39

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae R6

99.769

100

0.998
  comFA/cflA Streptococcus pneumoniae TIGR4

99.537

100

0.995
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

52.206

94.444

0.493
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387